5 Questions
What is the definition of sterilization?
Removal of all living organisms including bacteria and their spores
Which method is used for sterilization of dry material in a sealed container?
Hot air oven
What is the purpose of 2% NaHCO3 in the sterilization process?
Promote sterilization & prevent rusting
What is the principle of an autoclave for sterilization?
Heating under pressure
At what temperature and for how long is the hot air oven used for sterilization?
160°C for 2 hours, 180°C for 1 hour
Study Notes
Sterilization
- Sterilization is the process of eliminating or destroying all forms of microbial life, including bacterial spores, viruses, and fungi.
Sterilization Methods
- For dry material in a sealed container, the method used for sterilization is dry heat sterilization.
- Dry heat sterilization is achieved through the use of hot air oven.
Role of NaHCO3 in Sterilization
- 2% NaHCO3 is used to neutralize the alkalinity of the glassware and to maintain the pH of the medium, which is essential for microbial growth.
Autoclave Sterilization
- The principle of an autoclave for sterilization is based on the use of high-pressure steam to destroy microbial life.
Hot Air Oven Sterilization
- Hot air oven is used for sterilization at a temperature of 160°C for 2 hours or 180°C for 1 hour.
Test your knowledge of sterilization methods with this quiz. From heat to filtration to chemical and irradiation methods, this quiz covers the various techniques used to free articles from all living organisms, including bacteria and their spores. Perfect for medical microbiology and immunology students.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
