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Questions and Answers
What is the definition of sterilization?
What is the definition of sterilization?
- Killing of most pathogenic organisms but not all spores
- Removal of some pathogenic organisms but not spores
- Removal of all living organisms including bacteria and their spores (correct)
- Killing of all pathogenic organisms but not their spores
Which method is used for sterilization of dry material in a sealed container?
Which method is used for sterilization of dry material in a sealed container?
- Hot air oven (correct)
- Autoclave
- Boiling at 100°C
- Pasteurization
What is the purpose of 2% NaHCO3 in the sterilization process?
What is the purpose of 2% NaHCO3 in the sterilization process?
- Promote sterilization & prevent rusting (correct)
- Prevent contamination
- Kill spores
- Enhance heat conductivity
What is the principle of an autoclave for sterilization?
What is the principle of an autoclave for sterilization?
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At what temperature and for how long is the hot air oven used for sterilization?
At what temperature and for how long is the hot air oven used for sterilization?
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Study Notes
Sterilization
- Sterilization is the process of eliminating or destroying all forms of microbial life, including bacterial spores, viruses, and fungi.
Sterilization Methods
- For dry material in a sealed container, the method used for sterilization is dry heat sterilization.
- Dry heat sterilization is achieved through the use of hot air oven.
Role of NaHCO3 in Sterilization
- 2% NaHCO3 is used to neutralize the alkalinity of the glassware and to maintain the pH of the medium, which is essential for microbial growth.
Autoclave Sterilization
- The principle of an autoclave for sterilization is based on the use of high-pressure steam to destroy microbial life.
Hot Air Oven Sterilization
- Hot air oven is used for sterilization at a temperature of 160°C for 2 hours or 180°C for 1 hour.
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