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Questions and Answers
What is an advantage of using monoclonal antibodies?
What is an advantage of using monoclonal antibodies?
Which type of antibody is typically used in immunoassays?
Which type of antibody is typically used in immunoassays?
What is a limitation of using polyclonal antibodies?
What is a limitation of using polyclonal antibodies?
Why are polyclonal antibodies often used for detecting large antigens?
Why are polyclonal antibodies often used for detecting large antigens?
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What is a characteristic of monoclonal antibody production?
What is a characteristic of monoclonal antibody production?
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What is a key difference in the source of monoclonal and polyclonal antibodies?
What is a key difference in the source of monoclonal and polyclonal antibodies?
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What is a consequence of using polyclonal antibodies from different animals?
What is a consequence of using polyclonal antibodies from different animals?
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Why are monoclonal antibodies often used in immunoassays?
Why are monoclonal antibodies often used in immunoassays?
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What is a characteristic of antigens that are often detected using polyclonal antibodies?
What is a characteristic of antigens that are often detected using polyclonal antibodies?
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What is a requirement for producing polyclonal antibodies?
What is a requirement for producing polyclonal antibodies?
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Study Notes
Monoclonal Antibodies
- Restricted to mouse cells
- Can be developed and grown in cell culture, which is quick and inexpensive
- Provides a lifelong source of antibodies with minimal purification needed
- Most commonly used in immunoassays
- Offers a constant source of antibodies
Polyclonal Antibodies
- Can be obtained from various animal species, as long as they are large enough
- Requires animal housing and strict regulations
- Provides a finite source of antibodies, as the animal will eventually die of old age
- The antibody source changes each time a new animal is used
- Requires purification of antibodies from other cell components
- Best suited for large antigens with changing epitopes, such as bacteria
Monoclonal Antibodies
- Restricted to mouse cells
- Can be developed and grown in cell culture, which is quick and inexpensive
- Provides a lifelong source of antibodies with minimal purification needed
- Most commonly used in immunoassays
- Offers a constant source of antibodies
Polyclonal Antibodies
- Can be obtained from various animal species, as long as they are large enough
- Requires animal housing and strict regulations
- Provides a finite source of antibodies, as the animal will eventually die of old age
- The antibody source changes each time a new animal is used
- Requires purification of antibodies from other cell components
- Best suited for large antigens with changing epitopes, such as bacteria
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Description
Compare and contrast monoclonal and polyclonal antibodies, including their sources, advantages, and uses. Learn about their differences in development, purification, and applications.
