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Questions and Answers
What is an advantage of using monoclonal antibodies?
Which type of antibody is typically used in immunoassays?
What is a limitation of using polyclonal antibodies?
Why are polyclonal antibodies often used for detecting large antigens?
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What is a characteristic of monoclonal antibody production?
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What is a key difference in the source of monoclonal and polyclonal antibodies?
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What is a consequence of using polyclonal antibodies from different animals?
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Why are monoclonal antibodies often used in immunoassays?
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What is a characteristic of antigens that are often detected using polyclonal antibodies?
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What is a requirement for producing polyclonal antibodies?
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Study Notes
Monoclonal Antibodies
- Restricted to mouse cells
- Can be developed and grown in cell culture, which is quick and inexpensive
- Provides a lifelong source of antibodies with minimal purification needed
- Most commonly used in immunoassays
- Offers a constant source of antibodies
Polyclonal Antibodies
- Can be obtained from various animal species, as long as they are large enough
- Requires animal housing and strict regulations
- Provides a finite source of antibodies, as the animal will eventually die of old age
- The antibody source changes each time a new animal is used
- Requires purification of antibodies from other cell components
- Best suited for large antigens with changing epitopes, such as bacteria
Monoclonal Antibodies
- Restricted to mouse cells
- Can be developed and grown in cell culture, which is quick and inexpensive
- Provides a lifelong source of antibodies with minimal purification needed
- Most commonly used in immunoassays
- Offers a constant source of antibodies
Polyclonal Antibodies
- Can be obtained from various animal species, as long as they are large enough
- Requires animal housing and strict regulations
- Provides a finite source of antibodies, as the animal will eventually die of old age
- The antibody source changes each time a new animal is used
- Requires purification of antibodies from other cell components
- Best suited for large antigens with changing epitopes, such as bacteria
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Description
Compare and contrast monoclonal and polyclonal antibodies, including their sources, advantages, and uses. Learn about their differences in development, purification, and applications.
