16 Questions
What is the main purpose of hybridoma technology?
To produce monoclonal antibodies
What is the advantage of using hybridoma technology over other methods?
Cost-effectiveness
What is the importance of monoclonal antibodies in research?
They are used in various fields of research
What is the limitation of monoclonal antibodies produced by hybridoma technology?
They pose a risk of disease transfer from mice to humans
How are monoclonal antibodies produced using hybridoma technology?
By fusing B lymphocytes with immortal myeloma cell lines
What is the advantage of monoclonal antibodies produced by hybridoma technology?
They are highly specific and sensitive
What is the use of radiolabeled monoclonal antibodies?
To detect tumor antigens in living systems
What has helped in reducing the limitations of hybridoma technology?
Genetic engineering
What is the primary purpose of producing antibodies?
For therapeutic and diagnostic applications
Who discovered the technique of hybridoma technology?
Milstein and Kohler
What is the result of fusing antibody-producing B cells with immortal cancerous cell lines?
Formation of an immortal hybrid cell line
What is the advantage of hybridoma technology?
It produces highly specific antibodies
What is cryopreservation used for in hybridoma technology?
To preserve hybridoma cells for a long period of time
What type of cancerous cell lines are used in hybridoma technology?
Myeloma cells
What are monoclonal antibodies specific to?
Specific antigens
What is the significance of hybridoma technology?
It is a major turning point in the field of biotechnology
Study Notes
Hybridoma Technology
- Produces monoclonal antibodies (mAbs) specific to antigens
- Involves fusing antibody-producing B lymphocytes with immortal myeloma cell lines to form hybrid cells (hybridoma cell lines)
- Hybridoma cells are cultured in a lab to produce monoclonal antibodies against a specific antigen
Advantages of Hybridoma Technology
- Antibodies produced are of high purity, highly sensitive, and specific
- Cost-effective
- Offers limitless production of homogenized antibodies
- Important tool in various research fields, including toxicology, animal biotechnology, medicine, pharmacology, cell, and molecular biology
Applications of Monoclonal Antibodies
- Used in diagnostic, imaging, and therapeutic purposes
- Radiolabeled monoclonal antibodies are used as probes to detect tumor antigens in the living system
- Radioisotope coupled antibodies are used for therapeutic target-specific action on oncogenic cells
Challenges and Limitations
- Mice or rat-derived monoclonal antibodies pose a risk of disease transfer to humans
- No guarantee of virus-free antibodies, despite purification
- Immunogenic responses observed against mouse-origin antibodies
Future of Monoclonal Antibody Production
- Advanced techniques, such as genetic engineering, aim to reduce limitations
- Development of lab-produced monoclonal antibodies with human-like properties
- Cryopreservation of hybridoma cells for long-term storage
This quiz covers the process of producing monoclonal antibodies using hybridoma technology, including the isolation of B lymphocytes, fusion with myeloma cells, and culturing of hybridoma cells.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
