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Questions and Answers
What is the main purpose of hybridoma technology?
What is the main purpose of hybridoma technology?
- To produce vaccines
- To produce antigens
- To produce polyclonal antibodies
- To produce monoclonal antibodies (correct)
What is the advantage of using hybridoma technology over other methods?
What is the advantage of using hybridoma technology over other methods?
- Production of antibodies with low purity
- Production of antibodies with low specificity
- Cost-effectiveness (correct)
- Production of polyclonal antibodies
What is the importance of monoclonal antibodies in research?
What is the importance of monoclonal antibodies in research?
- They are used in diagnostic purposes only
- They are used in imaging purposes only
- They are used in therapeutic purposes only
- They are used in various fields of research (correct)
What is the limitation of monoclonal antibodies produced by hybridoma technology?
What is the limitation of monoclonal antibodies produced by hybridoma technology?
How are monoclonal antibodies produced using hybridoma technology?
How are monoclonal antibodies produced using hybridoma technology?
What is the advantage of monoclonal antibodies produced by hybridoma technology?
What is the advantage of monoclonal antibodies produced by hybridoma technology?
What is the use of radiolabeled monoclonal antibodies?
What is the use of radiolabeled monoclonal antibodies?
What has helped in reducing the limitations of hybridoma technology?
What has helped in reducing the limitations of hybridoma technology?
What is the primary purpose of producing antibodies?
What is the primary purpose of producing antibodies?
Who discovered the technique of hybridoma technology?
Who discovered the technique of hybridoma technology?
What is the result of fusing antibody-producing B cells with immortal cancerous cell lines?
What is the result of fusing antibody-producing B cells with immortal cancerous cell lines?
What is the advantage of hybridoma technology?
What is the advantage of hybridoma technology?
What is cryopreservation used for in hybridoma technology?
What is cryopreservation used for in hybridoma technology?
What type of cancerous cell lines are used in hybridoma technology?
What type of cancerous cell lines are used in hybridoma technology?
What are monoclonal antibodies specific to?
What are monoclonal antibodies specific to?
What is the significance of hybridoma technology?
What is the significance of hybridoma technology?
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Study Notes
Hybridoma Technology
- Produces monoclonal antibodies (mAbs) specific to antigens
- Involves fusing antibody-producing B lymphocytes with immortal myeloma cell lines to form hybrid cells (hybridoma cell lines)
- Hybridoma cells are cultured in a lab to produce monoclonal antibodies against a specific antigen
Advantages of Hybridoma Technology
- Antibodies produced are of high purity, highly sensitive, and specific
- Cost-effective
- Offers limitless production of homogenized antibodies
- Important tool in various research fields, including toxicology, animal biotechnology, medicine, pharmacology, cell, and molecular biology
Applications of Monoclonal Antibodies
- Used in diagnostic, imaging, and therapeutic purposes
- Radiolabeled monoclonal antibodies are used as probes to detect tumor antigens in the living system
- Radioisotope coupled antibodies are used for therapeutic target-specific action on oncogenic cells
Challenges and Limitations
- Mice or rat-derived monoclonal antibodies pose a risk of disease transfer to humans
- No guarantee of virus-free antibodies, despite purification
- Immunogenic responses observed against mouse-origin antibodies
Future of Monoclonal Antibody Production
- Advanced techniques, such as genetic engineering, aim to reduce limitations
- Development of lab-produced monoclonal antibodies with human-like properties
- Cryopreservation of hybridoma cells for long-term storage
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