Hybridoma Technology in Monoclonal Antibody Production

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Questions and Answers

What is the main purpose of hybridoma technology?

To produce vaccines

To produce antigens

To produce polyclonal antibodies

To produce monoclonal antibodies (correct)

What is the advantage of using hybridoma technology over other methods?

Production of antibodies with low purity

Production of antibodies with low specificity

Cost-effectiveness (correct)

Production of polyclonal antibodies

What is the importance of monoclonal antibodies in research?

They are used in diagnostic purposes only

They are used in imaging purposes only

They are used in therapeutic purposes only

They are used in various fields of research (correct)

What is the limitation of monoclonal antibodies produced by hybridoma technology?

They pose a risk of disease transfer from mice to humans Signup and view all the answers

How are monoclonal antibodies produced using hybridoma technology?

By fusing B lymphocytes with immortal myeloma cell lines Signup and view all the answers

What is the advantage of monoclonal antibodies produced by hybridoma technology?

They are highly specific and sensitive Signup and view all the answers

What is the use of radiolabeled monoclonal antibodies?

To detect tumor antigens in living systems Signup and view all the answers

What has helped in reducing the limitations of hybridoma technology?

Genetic engineering Signup and view all the answers

What is the primary purpose of producing antibodies?

For therapeutic and diagnostic applications Signup and view all the answers

Who discovered the technique of hybridoma technology?

Milstein and Kohler Signup and view all the answers

What is the result of fusing antibody-producing B cells with immortal cancerous cell lines?

Formation of an immortal hybrid cell line Signup and view all the answers

What is the advantage of hybridoma technology?

It produces highly specific antibodies Signup and view all the answers

What is cryopreservation used for in hybridoma technology?

To preserve hybridoma cells for a long period of time Signup and view all the answers

What type of cancerous cell lines are used in hybridoma technology?

Myeloma cells Signup and view all the answers

What are monoclonal antibodies specific to?

Specific antigens Signup and view all the answers

What is the significance of hybridoma technology?

It is a major turning point in the field of biotechnology Signup and view all the answers

Study Notes

Hybridoma Technology

Produces monoclonal antibodies (mAbs) specific to antigens
Involves fusing antibody-producing B lymphocytes with immortal myeloma cell lines to form hybrid cells (hybridoma cell lines)
Hybridoma cells are cultured in a lab to produce monoclonal antibodies against a specific antigen

Advantages of Hybridoma Technology

Antibodies produced are of high purity, highly sensitive, and specific
Cost-effective
Offers limitless production of homogenized antibodies
Important tool in various research fields, including toxicology, animal biotechnology, medicine, pharmacology, cell, and molecular biology

Applications of Monoclonal Antibodies

Used in diagnostic, imaging, and therapeutic purposes
Radiolabeled monoclonal antibodies are used as probes to detect tumor antigens in the living system
Radioisotope coupled antibodies are used for therapeutic target-specific action on oncogenic cells

Challenges and Limitations

Mice or rat-derived monoclonal antibodies pose a risk of disease transfer to humans
No guarantee of virus-free antibodies, despite purification
Immunogenic responses observed against mouse-origin antibodies

Future of Monoclonal Antibody Production

Advanced techniques, such as genetic engineering, aim to reduce limitations
Development of lab-produced monoclonal antibodies with human-like properties
Cryopreservation of hybridoma cells for long-term storage

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Description

This quiz covers the process of producing monoclonal antibodies using hybridoma technology, including the isolation of B lymphocytes, fusion with myeloma cells, and culturing of hybridoma cells.