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Questions and Answers
A solid medium is primarily used for isolating pure ______.
A solid medium is primarily used for isolating pure ______.
cultures
A semi solid medium is useful for demonstrating ______.
A semi solid medium is useful for demonstrating ______.
motility
Cloudiness in a liquid medium indicates ______.
Cloudiness in a liquid medium indicates ______.
growth
Anaerobic media are used to cultivate bacteria in ______ oxygen conditions.
Anaerobic media are used to cultivate bacteria in ______ oxygen conditions.
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Simple media are general purpose and support the growth of ______ microbes.
Simple media are general purpose and support the growth of ______ microbes.
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Complex media, like blood agar, have ingredients with ______ components.
Complex media, like blood agar, have ingredients with ______ components.
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Synthetic media are made from pure chemical ______.
Synthetic media are made from pure chemical ______.
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Study Notes
Types of Culture Media
- Culture media are used to grow microorganisms in the lab.
- Culture media can be classified based on their physical state, the presence of oxygen, or their nutritional factors.
Classification based on physical state
- Solid media are used to isolate pure cultures.
- Semi-solid media are used to demonstrate motility.
- Liquid media are used to grow large quantities of microorganisms uniformly.
Classification based on the presence and absence of oxygen
- Anaerobic media are used to cultivate anaerobic bacteria under low oxygen conditions.
- Aerobic media support growth of microbes on solid media.
Classification based on nutritional factors
- Simple media (basal media) are general purpose media that support the growth of non-fastidious microbes.
- Complex media contain components that are difficult to estimate the exact composition of, such as blood agar.
- Synthetic or defined media are specifically prepared using pure chemical substances for research purposes.
- Special media are used to isolate specific types of microorganisms.
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