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Questions and Answers
What is the primary purpose of using inhibitory agents in culture media?
What is the primary purpose of using inhibitory agents in culture media?
Which physical state of culture medium is commonly referred to as broth?
Which physical state of culture medium is commonly referred to as broth?
What amount of molten agar is typically transferred to a sterile Petri plate for culture?
What amount of molten agar is typically transferred to a sterile Petri plate for culture?
Which type of culture media is designed to support a wide range of microbial growth?
Which type of culture media is designed to support a wide range of microbial growth?
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Which of the following is NOT a component of culture medium?
Which of the following is NOT a component of culture medium?
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What describes the appearance and growth characteristics of a colony on a culture plate?
What describes the appearance and growth characteristics of a colony on a culture plate?
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Which type of culture medium is used to specifically favor the production of certain compounds?
Which type of culture medium is used to specifically favor the production of certain compounds?
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When reporting growth on plates, which aspect is typically assessed?
When reporting growth on plates, which aspect is typically assessed?
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Which of the following is a key component of solid culture media?
Which of the following is a key component of solid culture media?
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What is the purpose of using a sulfide indole motility (SIM) medium?
What is the purpose of using a sulfide indole motility (SIM) medium?
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In which type of media is the organism's motility typically observed?
In which type of media is the organism's motility typically observed?
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What is the function of plated media in microbiological culture?
What is the function of plated media in microbiological culture?
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Which of the following cell lines is derived from laryngeal carcinoma?
Which of the following cell lines is derived from laryngeal carcinoma?
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Which media type is primarily used for detecting aerobes, anaerobes, and microaerophiles?
Which media type is primarily used for detecting aerobes, anaerobes, and microaerophiles?
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What is crucial for obtaining isolated colonies from a microbial sample?
What is crucial for obtaining isolated colonies from a microbial sample?
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Which culture media type is distributed in sterile test tubes?
Which culture media type is distributed in sterile test tubes?
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Which characteristic describes the colonial morphology of Nocardia spp.?
Which characteristic describes the colonial morphology of Nocardia spp.?
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Which of the following options is a feature of Pseudomonas aeruginosa on culture media?
Which of the following options is a feature of Pseudomonas aeruginosa on culture media?
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What type of odor is associated with Staphylococcus aureus when grown on Mannitol Salt Agar?
What type of odor is associated with Staphylococcus aureus when grown on Mannitol Salt Agar?
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What is a common colonial morphology characteristic of β-Hemolytic streptococci except group B?
What is a common colonial morphology characteristic of β-Hemolytic streptococci except group B?
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Serratia marcescens exhibits what color at room temperature?
Serratia marcescens exhibits what color at room temperature?
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What is the expected colony morphology of Kluyvera spp.?
What is the expected colony morphology of Kluyvera spp.?
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Which odor is described for Haemophilus spp. on culture media?
Which odor is described for Haemophilus spp. on culture media?
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What term best describes the opacity characteristics of most gram-negative rods on culture media?
What term best describes the opacity characteristics of most gram-negative rods on culture media?
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Study Notes
Culture Medium
- A nutrient material for the growth and cultivation of microorganisms in the lab.
- Composed of a mixture of nutrients: carbon, nitrogen, sulfur, phosphorus, hydrogen, oxygen, and a buffer.
- Uses: growth and maintenance of microbial culture, production of particular compounds, and studying microbial action.
- Inhibitory agents can be used to isolate specific organisms by suppressing the growth of others.
Classification of Culture Media
According to Physical State or Consistency
Liquid Medium
- Commonly called broth.
- Does not contain agar.
- Used to determine organism motility, observe bacterial motility, and detect indole and sulfide production.
- Example: Sulfide Indole Motility (SIM) Medium.
Solid Medium
- Contains 1.5% to 2% agar as a solidifying agent.
- Does not inhibit bacteria or liquefy at room temperature.
- Used to develop surface colony growth of microorganisms and obtain isolated colonies.
- Examples: Triple Sugar Iron (TSI) Agar, MacConkey (MAC) Agar, Blood Agar Plate (BAP), Chocolate Agar Plate (CAP).
According to Composition
Tissue Culture Medium
- Contains living tissues.
- Used for obligate intracellular bacteria like Rickettsia and Chlamydia.
- Examples: McCoy Cells, Vero Cells, A549, HeLa Cells, Hep 2 Cells.
- Does not contain specific nutrients, incubated for a certain period and then subcultured to isolate the desired organism.
- Can be used as a supplement to agar plates for detection of aerobes, anaerobes, and microaerophiles.
- (THIOGLYCOLLATE)
Other Classifications
Density
- Translucent, semi-opaque, or opaque.
- Examples:
- β-Hemolytic streptococci except group B (S.agalactiae) – translucent
- S. Agalactiae - semi opaque
- Staphylococci and other gram-positive bacteria - opaque
- Most gram-negative rods are opaque
- Bordetella pertussis - shiny, like a half-pearl, on blood-containing media.
Pigment
- Pseudomonas aeruginosa - green
- Serratia marcescens – brick-red (especially at room temperature)
- Chromobacterium violaceum – purple
- Prevotella melaninogenica – brown black (anaerobic)
- Kluyvera spp. - blue
Colonial Morphology
- Staphylococci aureus: creamy
- Nocardia spp: brittle, crumbly and wrinkled, resembling breadcrumbs on a plate
- Diphtheroid: dry and waxy
Colonial Morphology (cont.)
Odor
- S. aureus - old sock (Mannitol Salt Agar)
- P. aeruginosa - fruity or grapelike
- Proteus mirabilis - putrid
- Haemophilus spp. - musty basement, “mousy” or “mouse nest” smell
- Nocardia spp. - freshly plowed field
Ways to Facilitate Anaerobic Cultivation
- Decolorization of indicator strip:
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Description
This quiz covers the essential concepts surrounding culture media in microbiology, including their composition, uses, and classifications based on physical state. It delves into both liquid and solid media, highlighting their specific applications and examples. Test your knowledge on how culture media facilitate microbial growth and experimentation.
