Culture Media (Growth Media) PDF
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This document describes different types of culture media, categorizing them by physical state (solid, semi-solid, liquid), oxygen requirements (anaerobic, aerobic), and nutritional factors (simple, complex, synthetic). It highlights the various uses of each type in microbiology.
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- - - requirements. - **Types of culture media** **I. Classification based on physical state** **a)solid medium**- isolating of pure cultures - **b)semi solid medium**- demonstrates motility **c) liquid medium**- growing large quantities uniformly...
- - - requirements. - **Types of culture media** **I. Classification based on physical state** **a)solid medium**- isolating of pure cultures - **b)semi solid medium**- demonstrates motility **c) liquid medium**- growing large quantities uniformly \- cloudiness indicates growth **II. Classification based on the presence and absence of oxygen** **a) anaerobic media**- Cultivates anaerobic bacteria at low oxygen, reducing oxidation-reduction potential. **b) aerobic media**- Easy to cultivate microbes on solid media - - - **III. Classification based on nutritional factors** **a) simple media (basal media)** \- general purpose media that supports growth of non-fastidious microbes -primary used for the isolation of microorganisms **b) complex medium**- such as blood agar, it has ingredients that exact components are difficult to estimate. **c) synthetic or defined medium-** specially prepared media from pure chemical substances for research purpose and composition of every component is well known **d) Special media** - - - - -
