Transgenic Animal Technologies Quiz

Transgenic Animal Technologies and Applications

• Microinjection with a fine glass pipette is used to deliver a picoliter volume of a gene into embryos, and a viral vector can also be utilized for gene transformation.
• Fertilized eggs are cultured in vitro to support physiological and metabolic processes, enabling various in vitro manipulations such as introducing foreign DNA through techniques like microinjection and retroviral insertion.
• DNA microinjection involves using micromanipulators to inject recombinant DNA into embryos, offering advantages like high transgenic animal generation frequency but facing limitations such as positional effects and potential mutagenesis.
• Animals produced using DNA microinjection must be rigorously tested to confirm transgene presence in all cells and the production of the gene's protein product.
• Retrovirus-mediated gene transfer achieves high rates of gene transfer but has limitations on foreign DNA insert size and potential for undesired genetic recombination.
• Transgenic animal models are extensively used to study human diseases and assess therapeutic applications, such as in Alzheimer's disease research.
• Transgenic animals are employed for the production of therapeutic proteins, like ATryn, the first transgenically produced therapeutic protein approved by the U.S. FDA.
• Xenotransplantation involves the transplantation of living cells, tissues, or organs from one species to another, facing immunological barriers that can be overcome through knockout engineered pigs and transgenic pigs.
• Xenotransplantation is considered as a solution to the shortage of human donors for organ transplants, particularly for kidney, liver, heart, and islet cell transplantation for diabetes.
• Cloning and transgenesis are distinct techniques, with animal cloning resulting from nuclear transfer technology, and Dolly the sheep being an example of animal cloning without possessing a transgene.
• Transgenic plants are generated in laboratories by altering their genetic makeup through methods like biolistic particle gun or Agrobacterium tumefaciens bacteria.

Explore the world of transgenic animal technologies and applications with this quiz. Test your knowledge on DNA microinjection, retrovirus-mediated gene transfer, transgenic animal models for disease research, xenotransplantation, and cloning. Learn about the production of therapeutic proteins and the genetic alteration of plants.