Animal Biotechnology: Cell Culture and Transgenic Animals

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What is the primary importance of animal cell culture?

It provides a controlled environment for the growth and study of animal cells.

What is the purpose of serum-free media in animal cell culturing?

To eliminate the need for animal-derived serum, reducing the risk of contamination and ensuring a more defined culture environment.

What is the main difference between primary and secondary cell cultures?

Primary cell cultures are derived directly from animal tissues, while secondary cell cultures are derived from primary cultures.

What is the purpose of cell growth kinetics in animal cell culture?

To understand and control cell growth rates, ensuring optimal cell density and productivity.

What is the main application of transgenic animals in biotechnology?

Generating transgenic animals that can produce recombinant proteins or exhibit specific traits.

What is the principle behind gene editing technologies such as TALEN and CRISPR/Cas9?

They enable precise modification of genomic sequences by targeting specific DNA sequences.

What is the primary purpose of micromanipulation technology in breeding of farm animals?

To enrich X and Y bearing sperms from semen samples and perform artificial insemination and germ cell manipulations.

What is the significance of aseptic techniques in cell culture?

To prevent contamination and ensure the growth of desired cells.

What is the purpose of recombinant cytokines in the treatment of diseases?

To provide targeted therapy for specific diseases.

What is the importance of biosafety guidelines in the release of transgenic animals?

To prevent environmental harm and ensure safe release of transgenic animals.

What is the role of embryonic stem cells in biotechnology?

To provide a source of cells for various applications, including regenerative medicine and tissue engineering.

What is the significance of nuclear transplantation in cloning?

To transfer genetic material from one cell to another, resulting in the creation of a cloned organism.

What is the title of the handbook written by Subbaram N.R. that discusses Indian patent law and practice?

Handbook of Indian Patent Law and Practice

Which edition of the book 'Culture of Animal Cells' by Freshney R.I. was published in 2010?

6th Edition

What is the name of the organization that published the background document for a workshop on biosafety issues related to genetically modified organisms?

DBT

How many marks are allocated to Part A of the end-semester examination question paper?

20

What is the total duration of the end-semester examination?

3 hours

Explore the fundamentals of animal cell culture and its applications, including micromanipulation and transgenic animals. Learn about the history and development of cell culture, basic tissue culture techniques, and serum-free media for animal cell culturing.

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