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Questions and Answers
What is the sugar component in ribonucleotides?
What is the sugar component in ribonucleotides?
Which of the following is NOT a difference between DNA and RNA?
Which of the following is NOT a difference between DNA and RNA?
What type of bond links nucleotides together in an RNA molecule?
What type of bond links nucleotides together in an RNA molecule?
What is the purpose of the central dogma of molecular biology?
What is the purpose of the central dogma of molecular biology?
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Which of the following is a key structural difference between DNA and RNA?
Which of the following is a key structural difference between DNA and RNA?
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What is the main function of RNA polymerase?
What is the main function of RNA polymerase?
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Which of the following is an example of an RNA molecule with a catalytic role?
Which of the following is an example of an RNA molecule with a catalytic role?
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Which of the following processes describes the synthesis of RNA from a DNA template?
Which of the following processes describes the synthesis of RNA from a DNA template?
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What is the process called that produces many identical RNA copies from the same gene?
What is the process called that produces many identical RNA copies from the same gene?
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What is the role of RNA in protein synthesis?
What is the role of RNA in protein synthesis?
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Which of the following strands of DNA serves as a template for transcription?
Which of the following strands of DNA serves as a template for transcription?
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What is the significance of a cell being able to express different genes at different rates?
What is the significance of a cell being able to express different genes at different rates?
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What is the relationship between transcription and translation?
What is the relationship between transcription and translation?
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What explains the directionality of transcription?
What explains the directionality of transcription?
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Which of these statements about eukaryotic transcription is true?
Which of these statements about eukaryotic transcription is true?
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What is the role of the sigma factor in bacterial transcription?
What is the role of the sigma factor in bacterial transcription?
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What is the function of a bacterial terminator?
What is the function of a bacterial terminator?
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Which of the following statements is true about the direction of transcription?
Which of the following statements is true about the direction of transcription?
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What is the role of phosphorylation of the RNA polymerase II tail in the transcription process?
What is the role of phosphorylation of the RNA polymerase II tail in the transcription process?
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Which protein complex plays a crucial role in opening the DNA double helix at the transcription start site?
Which protein complex plays a crucial role in opening the DNA double helix at the transcription start site?
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How do elongation factors contribute to the transcription process?
How do elongation factors contribute to the transcription process?
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At which stage of mRNA processing does the capping of the 5' end of the RNA transcript occur?
At which stage of mRNA processing does the capping of the 5' end of the RNA transcript occur?
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What is the role of polyadenylation in the processing of eukaryotic mRNA?
What is the role of polyadenylation in the processing of eukaryotic mRNA?
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What key difference distinguishes eukaryotic from bacterial gene organization?
What key difference distinguishes eukaryotic from bacterial gene organization?
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What is the primary role of general transcription factors (TFs) in eukaryotic transcription?
What is the primary role of general transcription factors (TFs) in eukaryotic transcription?
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Which protein binds to the TATA box, causing a distortion in the DNA double helix?
Which protein binds to the TATA box, causing a distortion in the DNA double helix?
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How do eukaryotic genes differ from bacterial genes in terms of their location on DNA?
How do eukaryotic genes differ from bacterial genes in terms of their location on DNA?
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What is the functional equivalent of the sigma factor in bacterial transcription in eukaryotic cells?
What is the functional equivalent of the sigma factor in bacterial transcription in eukaryotic cells?
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What is the significance of the distortion in the DNA double helix caused by TATA-box Binding Protein?
What is the significance of the distortion in the DNA double helix caused by TATA-box Binding Protein?
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What is the role of chromatin in eukaryotic transcription?
What is the role of chromatin in eukaryotic transcription?
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Why is purified RNA Polymerase II unable to initiate transcription alone?
Why is purified RNA Polymerase II unable to initiate transcription alone?
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How does transcription differ from DNA replication?
How does transcription differ from DNA replication?
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What is the role of RNA polymerase in transcription?
What is the role of RNA polymerase in transcription?
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What is the difference between the length of a mature RNA and a replicated DNA molecule?
What is the difference between the length of a mature RNA and a replicated DNA molecule?
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What is the direction of RNA synthesis during transcription?
What is the direction of RNA synthesis during transcription?
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Why is the RNA strand released from the DNA template soon after synthesis?
Why is the RNA strand released from the DNA template soon after synthesis?
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What does the electron micrograph of ribosomal genes demonstrate?
What does the electron micrograph of ribosomal genes demonstrate?
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What is the function of the phosphodiester bond in the context of transcription?
What is the function of the phosphodiester bond in the context of transcription?
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Flashcards
Central Dogma
Central Dogma
The flow of genetic information from DNA to RNA to protein.
RNA
RNA
A single-stranded nucleic acid made of ribonucleotides, differing from DNA.
RNA Polymerases
RNA Polymerases
Enzymes that synthesize RNA from a DNA template during transcription.
Transcription
Transcription
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Phosphodiester Bond
Phosphodiester Bond
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Base Pairing in RNA
Base Pairing in RNA
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Single-Stranded RNA
Single-Stranded RNA
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Roles of RNA
Roles of RNA
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Gene
Gene
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Translation
Translation
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Rate of transcription
Rate of transcription
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Rate of translation
Rate of translation
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Promoter Structure
Promoter Structure
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Polarity in Promoters
Polarity in Promoters
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Eukaryotic RNA Polymerases
Eukaryotic RNA Polymerases
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Bacterial Transcription Initiation
Bacterial Transcription Initiation
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Direction of Transcription
Direction of Transcription
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Transcription Initiation Complex
Transcription Initiation Complex
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TFIIH
TFIIH
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Elongation Factors (EFs)
Elongation Factors (EFs)
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Eukaryotic mRNA Processing
Eukaryotic mRNA Processing
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RNA Capping and Polyadenylation
RNA Capping and Polyadenylation
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General Transcription Factors
General Transcription Factors
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Promoter Assembly
Promoter Assembly
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TATA Box
TATA Box
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Eukaryotic Gene Layout
Eukaryotic Gene Layout
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Nucleosomes
Nucleosomes
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Transcriptional Regulation Complexity
Transcriptional Regulation Complexity
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RNA transcript
RNA transcript
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Complementary Ribonucleotides
Complementary Ribonucleotides
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5’-to-3’ direction
5’-to-3’ direction
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Limited region of DNA
Limited region of DNA
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Simultaneous RNA synthesis
Simultaneous RNA synthesis
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Study Notes
RNA Polymerases
- RNA polymerases catalyze RNA synthesis
- The formation of phosphodiester bonds is catalyzed by RNA polymerases
Transcription in Bacteria
- RNA polymerase binds tightly to DNA after recognizing a specific nucleotide sequence upstream of the transcription start site - a promoter region
- Bacterial RNA polymerase contains a subunit called sigma factor, which recognizes the promoter
- RNA polymerase opens up the DNA double helix in front of the promoter
- One of the two exposed DNA strands acts as a template for complementary base-pairing with incoming ribonucleotides
- RNA synthesis begins
- Sigma factor is released
- RNA synthesis continues
- Elongation continues until RNA polymerase reaches a second signal in the DNA, the terminator (or stop site)
- Termination and release of both completed RNA transcript and RNA polymerase
- Bacterial promoters have specific sequences. The sequence of a typical bacterial promoter contains specific sequences at positions -35 and -10 relative to the start site.
Eukaryotic Transcription
- Eukaryotic transcription initiation differs significantly from the process in bacteria. Bacteria use a single type of RNA polymerase, whereas eukaryotic cells employ three RNA polymerases.
- RNA polymerase I, RNA polymerase II, and RNA polymerase III are the three RNA polymerases in eukaryotes
- RNA Pol I transcribes most rRNA genes
- RNA Pol II transcribes all protein-coding genes and miRNA genes. It also transcribes genes for other non-coding RNAs (such as those involved in splicing)
- RNA Pol III transcribes tRNA genes, 5S rRNA genes, and genes for various other small RNAs
Eukaryotic Gene Transcription Initiation
- Eukaryotic RNA polymerases require the assistance of general transcription factors (GTFs)
- General transcription factors are accessory proteins that must assemble at each promoter along with the RNA polymerase before transcription can begin
Important Differences Between Bacterial and Eukaryotic Systems
- Bacterial genes tend to lie very close together, with short stretches of non-transcribed DNA between them
- In eukaryotes, individual genes are spread out along the DNA with large stretches of non-transcribed DNA between genes
- This architecture in eukaryotes allows a single gene to be controlled by a variety of regulatory DNA sequences scattered along the DNA. This creates a more complex form of transcriptional regulation.
- Eukaryotic DNA is packed into nucleosomes and higher-order chromatin structures.
Eukaryotic mRNA Processing
- RNA processing steps take place as the RNA is being synthesized.
- These steps include capping, splicing, and polyadenylation
- These steps are carried out by enzymes bound to the phosphorylated tail of RNA polymerase II
- Capping, splicing, and polyadenylation (adding a poly(A) tail) are examples of these processes.
Introns and Exons
- Introns are non-coding DNA sequences found within genes in eukaryotes.
- Exons are coding DNA sequences that are part of a gene.
- In eukaryotes, splicing removes introns from the pre-mRNA molecule. Exons are then joined together to form the mature mRNA molecule.
RNA Splicing
- Splicing happens concurrently with transcription
- Introns are removed and exons are stitched together in this process.
- Each transcript receives a poly(A) tail, a functional mRNA, ready to leave the nucleus.
- Introns contain special short nucleotide sequences.
- these sequences help the splicing machinery to cut out the intron in the form of a "lariat" structure
- Small nuclear RNA (snRNAs) and associated proteins form the spliceosome, which carries out the splicing process.
- The spliceosome assembles on the RNA molecule as it is being synthesized.
Alternative Splicing
- Transcripts of many eukaryotic genes can be spliced in different ways
- This allows production of different proteins from the same gene.
Mature mRNA Export
- Transport of mRNA from the nucleus to the cytoplasm is highly
- Only correctly-processed mRNAs are exported.
- Export-ready mRNA molecules require specific protein partners, including cap-binding proteins, poly(A)-binding proteins, and exon junction complexes
- Export is mediated by the nuclear pore complex
mRNA Degradation
- Single mRNA molecules can be translated into proteins many times
- The length of time an mRNA resides in the cytoplasm influences the amount of protein produced
- mRNA molecules are eventually degraded by ribonucleases (RNase) in the cytosol
- mRNA degradation is regulated by sequences within the 3' untranslated region (UTR)
Pseudogenes
- A pseudogene is a DNA sequence that is similar to a functional gene but contains mutations that prevent its proper expression.
- Pseudogenes often arise from the duplication of a functional gene, followed by the accumulation of damaging mutations in one copy.
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Description
This quiz explores the mechanism of transcription in bacteria, focusing on the role of RNA polymerases. It covers the steps of RNA synthesis, including the binding to DNA, the function of sigma factors, and the termination of transcription. Test your knowledge of bacterial promoters and the intricacies of RNA synthesis.