Bacterial Gene Expression and Transcription Process Quiz
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Questions and Answers

What is the main role of a bacterial promoter in transcription initiation?

  • Help RNA polymerase recognize the start of genes (correct)
  • Act as a site for translation initiation
  • Facilitate splicing of pre-mRNA molecules
  • Bind to tRNA molecules during transcription

Which component of the bacterial RNA polymerase holoenzyme is responsible for recognizing the start of genes?

  • Sigma factor (correct)
  • RNA polymerase
  • Core enzyme
  • Rho factor

During which phase of transcription does the RNA polymerase unwind the DNA and form a temporary RNA:DNA hybrid?

  • Termination
  • Processing
  • Initiation
  • Elongation (correct)

What is the function of a terminator in bacterial transcription?

<p>Mark the end of a gene for transcription termination (D)</p> Signup and view all the answers

How does factor-independent termination of transcription differ from rho-dependent termination in bacteria?

<p>Rho-dependent termination relies on a protein called rho, while factor-independent termination does not. (B)</p> Signup and view all the answers

What is the significance of polycistronic mRNA often found in bacteria?

<p>It contains directions for multiple polypeptides synthesized by a single RNA polymerase (B)</p> Signup and view all the answers

What is the role of a bacterial promoter?

<p>Orients RNA polymerase for transcription (B)</p> Signup and view all the answers

Which enzyme is responsible for termination of transcription in bacteria?

<p>Rho factor (D)</p> Signup and view all the answers

During transcription in bacteria, what is the function of a sigma factor?

<p>Helps RNA polymerase recognize the promoter region (A)</p> Signup and view all the answers

What happens during the elongation phase of bacterial transcription?

<p>RNA polymerase synthesizes the RNA transcript in the 5' to 3' direction (C)</p> Signup and view all the answers

What event characterizes the termination phase of bacterial transcription?

<p>The formation of a hairpin loop in the mRNA (C)</p> Signup and view all the answers

Which enzyme helps unwind the DNA double helix during transcription initiation in bacteria?

<p>Helicase (D)</p> Signup and view all the answers

What is the role of the Shine-Dalgarno sequence in bacterial translation initiation?

<p>It aligns with the 16S rRNA of the ribosome. (B)</p> Signup and view all the answers

Which molecule catalyzes the formation of peptide bonds during bacterial translation?

<p>23S rRNA (C)</p> Signup and view all the answers

During translation elongation, what occurs at the A site of the bacterial ribosome?

<p>Transpeptidation reaction (B)</p> Signup and view all the answers

Which factor is required for the termination of protein synthesis in prokaryotes?

<p>Release factors (D)</p> Signup and view all the answers

What is the function of the N-formylmethionine-tRNA in bacteria during translation initiation?

<p>Aligns with 16S rRNA (B)</p> Signup and view all the answers

Which site on the bacterial ribosome binds the initiator tRNA or tRNA attached to the growing polypeptide?

<p>Peptidyl (donor; P) site (C)</p> Signup and view all the answers

What is responsible for catalyzing peptide bond formation during translation in bacteria?

<p>Peptidyl transferase of 23S rRNA (C)</p> Signup and view all the answers

Which component of the ribosome moves from the A site to the P site during translation elongation?

<p>$\text{tRNA}$ (C)</p> Signup and view all the answers

What is the significance of GTP hydrolysis during translation?

<p>$\text{Required for ribosome movement and translocation}$ (C)</p> Signup and view all the answers

What occurs when release factors recognize a stop codon during termination of protein synthesis?

<p>Release of the polypeptide chain from the ribosome (A)</p> Signup and view all the answers

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