10 Questions
What is the main reason MS medium is widely used in plant tissue culture work?
MS medium is successful with several plant species and culture systems.
Which medium was originally designed for cell suspension and callus cultures but is now also used for protoplast culture with modifications?
B5 medium
What is N6 medium primarily used for in plant tissue culture?
Cereal anther culture
What defines a synthetic medium in plant tissue culture?
A medium composed of chemically defined components
What is the main difference between synthetic and natural media in plant tissue culture?
Synthetic media contain chemically defined components, while natural media contain chemically undefined compounds.
Who developed Nitsch's medium, which is frequently used for anther cultures?
Nitsch and Nitsch
What is the purpose of White's medium in plant tissue culture?
Developed for root culture
What type of cultures was MS medium originally formulated to induce?
Organogenesis and regeneration of plants in cultured tissues
Which medium is most frequently used for many types of culture systems in plant tissue culture?
MS medium
How are concentrations of inorganic and organic constituents in culture media usually expressed?
As mass values like mg/l or ppm
Learn about the essential facilities required to set up a tissue culture lab, including washing area, media preparation, aseptic transfer area, incubators, and nutrient media. Understand the importance of each facility in maintaining a sterile working environment for tissue culture techniques.
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