10 Questions
What was the primary purpose of devising tissue culture at the beginning of the twentieth century?
To study the behavior of animal cells free of systemic variations
Who is credited with the first successful tissue culture experiment involving frog embryo nerve fibre outgrowth in vitro?
Harrison
What is the key event associated with the development of tissue culture by Carrel and Burrows?
Explants of chick connective tissue; heart muscle contractile for 2-3 months
What is the primary aim of trypsinization and subculture of cells in tissue culture?
To propagate cells for further study
What was the initial focus of tissue culture when it was first devised?
Study the behavior of animal cells
What is a fundamental aspect of the culture environment for cells?
Cell adhesion molecules, extracellular matrix, cytoskeleton, and cell motility
Which source provides cells close to their in vivo state but is more demanding to culture?
Animal tissue
What is a characteristic of the microenvironment in cell culture?
Lack of heterogeneity and 3D architecture
What is a feature of cells obtained from a culture collection?
Well characterized in terms of growth, origin, and genetic trait
Which aspect is related to the control of cell proliferation?
Cell cycle
Study Notes
Origins of Tissue Culture
- The primary purpose of devising tissue culture at the beginning of the twentieth century was to study the behavior of cells outside of the organism.
Ross Harrison's Contribution
- Ross Harrison is credited with the first successful tissue culture experiment involving frog embryo nerve fibre outgrowth in vitro.
Carrel and Burrows' Development
- The key event associated with the development of tissue culture by Carrel and Burrows was the use of a nutrient-rich medium to support cell growth.
Tissue Culture Techniques
- The primary aim of trypsinization and subculture of cells in tissue culture is to dissociate cells from the primary culture, and then re-seed them into a new culture vessel.
Initial Focus of Tissue Culture
- When first devised, the initial focus of tissue culture was on understanding embryonic development and tissue growth.
Culture Environment
- A fundamental aspect of the culture environment for cells is a controlled physical environment, including temperature, pH, and nutrient availability.
Cell Sources
- Primary cell cultures, derived from tissues or organs, provide cells close to their in vivo state, but are more demanding to culture.
Microenvironment
- A characteristic of the microenvironment in cell culture is the interaction between the cell and its surrounding environment, including the medium, surface, and other cells.
Cell Collections
- A feature of cells obtained from a culture collection is that they are typically well-characterized and have a known history.
Cell Proliferation
- The aspect related to the control of cell proliferation is the regulation of cell growth and division.
Test your knowledge of cell and tissue culture with this quiz! Assess your understanding of the background history, types, advantages, limitations, and applications of cell and tissue culture. This quiz is based on the lecture prepared by Assoc Prof. Dr. Tham Chau Ling from UPM.
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