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Questions and Answers
Which of the following is NOT a facility that a standard tissue culture lab should provide?
Which of the following is NOT a facility that a standard tissue culture lab should provide?
- Observation of culture (correct)
- Aseptic manipulation of plant material
- Preparations, sterilization and storage of nutrient medium
- Washing and storage of glassware, plastic wares and others
What is the aim of Experiment No. 1 (1A) in the plant tissue culture lab?
What is the aim of Experiment No. 1 (1A) in the plant tissue culture lab?
- Introduction to plant tissue culture (correct)
- Observation of culture
- Acclimatization of in-vitro developed plant
- Maintenance of culture under controlled conditions
What determines the size and exposure of a tissue culture set up in a lab?
What determines the size and exposure of a tissue culture set up in a lab?
- The availability of funds
- The number of separate labs or rooms available
- Both the nature of the project undertaken and the availability of funds (correct)
- The nature of the project undertaken
How many separate labs or rooms should be available for research work in a tissue culture lab?
How many separate labs or rooms should be available for research work in a tissue culture lab?
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What does the term 'tissue culture' refer to?
What does the term 'tissue culture' refer to?
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Study Notes
Tissue Culture Lab Facilities
- A standard tissue culture lab should provide facilities including a media preparation area, sterile area, microscope area, and storage area for chemicals and equipment.
Experiment No. 1 (1A) in Plant Tissue Culture Lab
- The aim of Experiment No. 1 (1A) is to initiate callus from explants and to establish aseptic conditions for tissue culture.
Size and Exposure of Tissue Culture Set-up
- The size and exposure of a tissue culture set-up in a lab are determined by the type of research, number of personnel, and the level of contamination risk.
Minimum Requirements for Research Work
- A minimum of four separate labs or rooms should be available for research work in a tissue culture lab: a transfer room, a sterile room, a media preparation room, and a storage room.
Definition of Tissue Culture
- Tissue culture refers to the growth of new plants or plant parts in a controlled environment, using a nutrient medium, under aseptic conditions.
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