Tissue Culture and Plant Growth in Vitro

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24 Questions

Which of the following is a primary energy source in tissue culture?

Sucrose

What is the definition of callus in plant tissue culture?

A disorganized mass of cells induced from explants through the application of auxin and cytokinin

What is the main application of protoplast culture in plant tissue culture?

DNA transformation and Hybrid plant production

Which of the following is NOT an application of animal cell culture?

Production of plant hormones

What is totipotency in plant tissue culture?

The inherent capacity of a plant cell to develop into an entire plant

What are the pros of tissue culture?

Allows for year-round propagation of plants

What factors influence the growth and development of cultured plants in tissue culture?

All of the above

Which method is used to introduce genes into plants via Agrobacterium?

Agrobacteria-mediated Transformation

What is the significance of genetic engineering in plant tissue culture?

It enables the introduction of specific traits into plants for desired characteristics

What is the effect of knocking out OsWRKY24 and OsWRKY53 genes in rice plants?

It improves drought tolerance

Which type of cells require a wider range of nutrients for survival in culture conditions?

Animal cells

Which of the following statements is true about plant tissue culture?

Plant cells can differentiate into various cell types within the plant body

What is the lifespan of second cultures compared to initial cultures?

Longer lifespan

What is the main application of plant tissue culture?

Artificial and micropropagation of plants

What is the main application of protoplast culture in plant tissue culture?

DNA transformation

What is the definition of callus in plant tissue culture?

A disorganized mass of cells

What is the significance of genetic engineering in plant tissue culture?

Manipulation of plant cells at the cellular and molecular levels

Which method is used to introduce genes into plants via Agrobacterium?

Transformation

Which of the following is an application of animal cell culture?

Development of vaccines

What is the inherent capacity of a plant cell to develop into an entire plant if suitably stimulated?

Totipotency

What are the pros of tissue culture?

Short growth period, year-round propagation, cultivation of challenging plant species

What factors influence the growth and development of cultured plants in tissue culture?

Culture media, explants source, aseptic conditions

What is the main purpose of the study mentioned in the text?

To investigate the role of OsWRKY24 and OsWRKY53 genes in drought stress response in rice

What is the significance of OsWRKY53 gene in long-term drought response in rice?

It does not significantly influence long-term drought response in rice

Test your knowledge on tissue culture and the factors that affect the success of plant growth in vitro. Explore the concept of species-dependent amenability and how factors like explant source and differentiation level can influence the outcome.

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