Plant Tissue Culture and Micropropagation

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What is the primary purpose of maintaining a controlled temperature of 25 ± 2°C in a growth room?

To induce callus formation

To stimulate shoot and root growth

To prevent culture contamination (correct)

To optimize nutrient absorption

What is the significance of fluorescence tubes and a timing device in a growth room?

To control humidity levels

To regulate nutrient diffusion

To maintain a sterile environment

To simulate day-night cycles (correct)

Why is it necessary to subculture callus tissues to fresh medium at regular intervals?

To induce necrosis

To prevent nutrient exhaustion (correct)

To increase humidity

To promote root growth

What is the primary characteristic of the log phase in plant tissue culture?

Rapid growth of the tissue Signup and view all the answers

What is the main reason behind the need to control humidity levels in a growth room?

To prevent culture contamination Signup and view all the answers

What is the primary indicator of necrosis in cultured tissues?

Decolorization on the surface of the explant Signup and view all the answers

What is the primary reason for performing all steps of tissue culture under aseptic conditions in a laminar flow cabinet?

To prevent growth of micro-organisms, which can outcompete the plant on nutrient media Signup and view all the answers

What is the purpose of adding growth hormones to the media in Stage I of micropropagation?

To accelerate the growth of the explant Signup and view all the answers

What is the purpose of sterilizing the explant using ethanol and sodium hypochlorite?

To prevent growth of micro-organisms on the explant Signup and view all the answers

What type of filter is used to sterilize solutions of thermolabile compounds?

Micropore membrane filter Signup and view all the answers

At what temperature is the culture media autoclaved in Stage 0 of micropropagation?

160°C Signup and view all the answers

What is the purpose of Stage IV of micropropagation?

To adapt the plantlet to the external environment Signup and view all the answers

What is the primary purpose of Stage II in micropropagation?

Shoot multiplication Signup and view all the answers

What is the ideal temperature range for the incubator chamber in Stage II?

20-24 °C Signup and view all the answers

What is the role of auxins in Stage III of micropropagation?

Rapid development of roots Signup and view all the answers

What is the purpose of subculturing callus in a liquid medium?

To produce secondary metabolites Signup and view all the answers

What is the difference between Stage III and Stage IV in micropropagation?

Stage III involves root development, while Stage IV involves plant adaptation Signup and view all the answers

What is the purpose of transferring plantlets from the lab to the greenhouse in Stage IV?

To allow plant adaptation to normal soil conditions Signup and view all the answers