22 Questions
What is the name of the scientist who developed the polymerase chain reaction (PCR) technique in 1985?
Kary Mullis
Which of the following is NOT a component required for the polymerase chain reaction (PCR) process?
DNA ligase
In the polymerase chain reaction (PCR) process, what is the purpose of the Taq polymerase enzyme?
To extend the daughter strands in a 5' to 3' direction using the dNTPs
In the Sanger sequencing technique, what is the purpose of the dideoxynucleotides (ddNTPs)?
To terminate the elongation of the daughter strands at every possible nucleotide
What is the purpose of the standardized ladder in gel electrophoresis?
To provide a reference for the sizes of the DNA fragments being analyzed
Which of the following was the first complete bacterial genome to be sequenced?
Haemophilus influenza
What is the main focus of Genomics?
Sequencing, interpreting, and comparing whole genomes
Why are many sequences referred to as Transcripts of Unknown Functions (TUFs)?
Their functions within the cell are not yet understood
What was the cost and duration of the Human Genome Project?
$2.7 billion over 13 years
How similar is the human genome to that of chimpanzees?
98.8% similar
What was the focus of Dr. Tallulah Andrews' research?
Analyzing single-cell RNAseq data
What is the primary objective of Genome Canada?
Funding and coordinating genomic projects in Canada
What is the purpose of Phase 3 in gene sequencing?
Annotating the sequences to find DNA that encodes genes, regulatory regions, and non-coding regions
Which process involves looking for typical sequences that code for promoters and regulatory sites?
Annotating the sequences to find gene regions
What is a common mutagen mentioned in the text?
Replication errors
Which enzyme detects and cleaves a mismatched nucleotide pair during DNA repair?
Nuclease
What type of mutation occurs with the substitution of a single nucleotide pair?
Point mutation
Which chromosomal mutation involves the loss of an entire chromosome fragment?
Deletions
'Trisomy 21' is associated with which genetic condition?
'Down' syndrome
'Sickle cell anemia' is caused by which type of mutation?
'Missense' mutation
'DNA uracil glycosylase enzyme' is involved in which type of DNA repair?
'Base excision' repair
'Reciprocal translocations' can lead to problems during gamete formation due to:
'Nondisjunction'
Explore the topic of In Vitro Replication and Polymerase Chain Reaction (PCR) including the components involved such as DNA sample, primers, dNTPs, and Taq polymerase. Learn about the process of PCR, how copies of DNA strands are produced, and the role of a thermocycler machine in the reaction.
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