Podcast
Questions and Answers
What is the name of the scientist who developed the polymerase chain reaction (PCR) technique in 1985?
What is the name of the scientist who developed the polymerase chain reaction (PCR) technique in 1985?
- Kary Mullis (correct)
- Thermus aquaticus
- Haemophilus influenza
- Frederick Sanger
Which of the following is NOT a component required for the polymerase chain reaction (PCR) process?
Which of the following is NOT a component required for the polymerase chain reaction (PCR) process?
- Short single-stranded DNA primers
- Sample of DNA
- DNA ligase (correct)
- Free deoxyribonucleotides (dNTPs)
In the polymerase chain reaction (PCR) process, what is the purpose of the Taq polymerase enzyme?
In the polymerase chain reaction (PCR) process, what is the purpose of the Taq polymerase enzyme?
- To extend the daughter strands in a 5' to 3' direction using the dNTPs (correct)
- To anneal the primers to the complementary sequences of the DNA template
- To label the dideoxynucleotides (ddNTPs) with fluorescent dyes
- To separate the strands of the double-stranded DNA
In the Sanger sequencing technique, what is the purpose of the dideoxynucleotides (ddNTPs)?
In the Sanger sequencing technique, what is the purpose of the dideoxynucleotides (ddNTPs)?
What is the purpose of the standardized ladder in gel electrophoresis?
What is the purpose of the standardized ladder in gel electrophoresis?
Which of the following was the first complete bacterial genome to be sequenced?
Which of the following was the first complete bacterial genome to be sequenced?
What is the main focus of Genomics?
What is the main focus of Genomics?
Why are many sequences referred to as Transcripts of Unknown Functions (TUFs)?
Why are many sequences referred to as Transcripts of Unknown Functions (TUFs)?
What was the cost and duration of the Human Genome Project?
What was the cost and duration of the Human Genome Project?
How similar is the human genome to that of chimpanzees?
How similar is the human genome to that of chimpanzees?
What was the focus of Dr. Tallulah Andrews' research?
What was the focus of Dr. Tallulah Andrews' research?
What is the primary objective of Genome Canada?
What is the primary objective of Genome Canada?
What is the purpose of Phase 3 in gene sequencing?
What is the purpose of Phase 3 in gene sequencing?
Which process involves looking for typical sequences that code for promoters and regulatory sites?
Which process involves looking for typical sequences that code for promoters and regulatory sites?
What is a common mutagen mentioned in the text?
What is a common mutagen mentioned in the text?
Which enzyme detects and cleaves a mismatched nucleotide pair during DNA repair?
Which enzyme detects and cleaves a mismatched nucleotide pair during DNA repair?
What type of mutation occurs with the substitution of a single nucleotide pair?
What type of mutation occurs with the substitution of a single nucleotide pair?
Which chromosomal mutation involves the loss of an entire chromosome fragment?
Which chromosomal mutation involves the loss of an entire chromosome fragment?
'Trisomy 21' is associated with which genetic condition?
'Trisomy 21' is associated with which genetic condition?
'Sickle cell anemia' is caused by which type of mutation?
'Sickle cell anemia' is caused by which type of mutation?
'DNA uracil glycosylase enzyme' is involved in which type of DNA repair?
'DNA uracil glycosylase enzyme' is involved in which type of DNA repair?
'Reciprocal translocations' can lead to problems during gamete formation due to:
'Reciprocal translocations' can lead to problems during gamete formation due to:
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