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Questions and Answers
What happens to initiation during in vitro transcription at low ionic strength?
What happens to initiation during in vitro transcription at low ionic strength?
- Initiation slows to a stop (correct)
- Initiation speeds up
- Initiation becomes more efficient
- Initiation remains constant
What is the effect of adding new core polymerase during in vitro transcription at low ionic strength?
What is the effect of adding new core polymerase during in vitro transcription at low ionic strength?
- Initiation stops permanently
- Initiation slows down further
- Initiation becomes irregular
- Initiation starts again (correct)
What must new core polymerase associate with during in vitro transcription at low ionic strength?
What must new core polymerase associate with during in vitro transcription at low ionic strength?
- Polymerase released from the original holoenzyme
- DNA released from the original holoenzyme
- RNA released from the original holoenzyme
- σ released from the original holoenzyme (correct)
What happens to the ribosomes when there is trp starvation?
What happens to the ribosomes when there is trp starvation?
What is the consequence of ribosomes pausing at the UGG codons?
What is the consequence of ribosomes pausing at the UGG codons?
What effect does trp starvation have on transcription and translation in bacteria?
What effect does trp starvation have on transcription and translation in bacteria?
What prevents the formation of the first hairpin (element 1 + 2) in the trp leader region under conditions of trp starvation?
What prevents the formation of the first hairpin (element 1 + 2) in the trp leader region under conditions of trp starvation?
Why does hairpin 2 + 3 form instead of the first hairpin (element 1 + 2) during trp starvation?
Why does hairpin 2 + 3 form instead of the first hairpin (element 1 + 2) during trp starvation?
What is the immediate consequence of ribosomes beginning to translate the mRNA as soon as the trp leader is transcribed?
What is the immediate consequence of ribosomes beginning to translate the mRNA as soon as the trp leader is transcribed?
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