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In Vitro Transcription and Core Polymerase

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In Vitro Transcription and Core Polymerase

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Questions and Answers

What happens to initiation during in vitro transcription at low ionic strength?

Initiation slows to a stop (correct)

Initiation speeds up

Initiation becomes more efficient

Initiation remains constant

What is the effect of adding new core polymerase during in vitro transcription at low ionic strength?

Initiation stops permanently

Initiation slows down further

Initiation becomes irregular

Initiation starts again (correct)

What must new core polymerase associate with during in vitro transcription at low ionic strength?

Polymerase released from the original holoenzyme

DNA released from the original holoenzyme

RNA released from the original holoenzyme

σ released from the original holoenzyme (correct)

What happens to the ribosomes when there is trp starvation?

They pause at the UGG codons in the leader region. Signup and view all the answers

What is the consequence of ribosomes pausing at the UGG codons?

Preventing the formation of the first hairpin. Signup and view all the answers

What effect does trp starvation have on transcription and translation in bacteria?

It causes ribosomes to begin translating the mRNA as soon as the trp leader is transcribed. Signup and view all the answers

What prevents the formation of the first hairpin (element 1 + 2) in the trp leader region under conditions of trp starvation?

The presence of ribosomes pausing at the UGG codons Signup and view all the answers

Why does hairpin 2 + 3 form instead of the first hairpin (element 1 + 2) during trp starvation?

Ribosomes prevent the formation of the first hairpin by pausing at the UGG codons Signup and view all the answers

What is the immediate consequence of ribosomes beginning to translate the mRNA as soon as the trp leader is transcribed?

Prevention of hairpin 1 + 2 formation Signup and view all the answers

In Vitro Transcription and Core Polymerase

Choose a study mode

Podcast

Questions and Answers

What happens to initiation during in vitro transcription at low ionic strength?

What is the effect of adding new core polymerase during in vitro transcription at low ionic strength?

What must new core polymerase associate with during in vitro transcription at low ionic strength?

What happens to the ribosomes when there is trp starvation?

What is the consequence of ribosomes pausing at the UGG codons?

What effect does trp starvation have on transcription and translation in bacteria?

What prevents the formation of the first hairpin (element 1 + 2) in the trp leader region under conditions of trp starvation?

Why does hairpin 2 + 3 form instead of the first hairpin (element 1 + 2) during trp starvation?

What is the immediate consequence of ribosomes beginning to translate the mRNA as soon as the trp leader is transcribed?

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