9 Questions
What happens to initiation during in vitro transcription at low ionic strength?
Initiation slows to a stop
What is the effect of adding new core polymerase during in vitro transcription at low ionic strength?
Initiation starts again
What must new core polymerase associate with during in vitro transcription at low ionic strength?
σ released from the original holoenzyme
What happens to the ribosomes when there is trp starvation?
They pause at the UGG codons in the leader region.
What is the consequence of ribosomes pausing at the UGG codons?
Preventing the formation of the first hairpin.
What effect does trp starvation have on transcription and translation in bacteria?
It causes ribosomes to begin translating the mRNA as soon as the trp leader is transcribed.
What prevents the formation of the first hairpin (element 1 + 2) in the trp leader region under conditions of trp starvation?
The presence of ribosomes pausing at the UGG codons
Why does hairpin 2 + 3 form instead of the first hairpin (element 1 + 2) during trp starvation?
Ribosomes prevent the formation of the first hairpin by pausing at the UGG codons
What is the immediate consequence of ribosomes beginning to translate the mRNA as soon as the trp leader is transcribed?
Prevention of hairpin 1 + 2 formation
Test your knowledge of in vitro transcription and the behavior of core polymerase at low ionic strength. Understand the phenomenon of initiation slowing down and restarting upon the addition of new core polymerase.
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