14 Questions
What is the meaning of 'in vitro'?
In glass
Who conducted the very first in vitro manipulation of eggs/embryos?
Walter Heape
Which journal published the paper titled 'Early stages of fertilization in vitro of human oocytes matured in vitro'?
Nature
What is the colloquial term for babies conceived as a result of IVF?
Test tube babies
In women with blocked fallopian tubes, what has largely replaced surgery as the treatment of choice?
IVF
Which condition often involves tubal involvement and ovarian cysts, and is a common indication for IVF?
Endometriosis
What is the purpose of ovarian stimulation in the IVF process?
To produce multiple mature follicles
What is the timing for egg retrieval after the injection of human chorionic gonadotropin (hCG)?
34-36 hours
Where are mature eggs placed after retrieval for IVF?
In a petri dish with culture medium
What is the purpose of collecting sperms before or after the oocyte collection?
To prepare for fertilization of the eggs
What is the purpose of incubating the collected sperms for 30-60 minutes at 37⁰ C?
To locate the most active sperms on the surface of the culture medium
In what circumstance might laparoscopy be used to retrieve eggs in the IVF process?
When one or both ovaries are not accessible by transvaginal ultrasound
What is the role of human chorionic gonadotropin (hCG) in the IVF process?
It triggers egg maturation for retrieval
What is the purpose of regular monitoring by ultrasound scan during ovarian stimulation?
To assess the response to ovarian stimulation
Study Notes
In Vitro Fertilization (IVF)
- "In vitro" means "in glass" or outside the body.
History of IVF
- The first in vitro manipulation of eggs/embryos was conducted by Robert Edwards and Patrick Steptoe.
Landmark Study
- The paper titled 'Early stages of fertilization in vitro of human oocytes matured in vitro' was published in a journal.
IVF Terminology
- Babies conceived as a result of IVF are colloquially known as "test tube babies."
Treatment of Blocked Fallopian Tubes
- In women with blocked fallopian tubes, IVF has largely replaced surgery as the treatment of choice.
Indications for IVF
- Endometriosis, which often involves tubal involvement and ovarian cysts, is a common indication for IVF.
Ovarian Stimulation in IVF
- The purpose of ovarian stimulation in the IVF process is to stimulate the ovaries to produce multiple eggs.
Egg Retrieval
- Egg retrieval typically occurs 34-36 hours after the injection of human chorionic gonadotropin (hCG).
- Mature eggs are placed in a culture medium after retrieval for IVF.
Sperm Collection and Preparation
- Sperm collection occurs before or after oocyte collection.
- The purpose of incubating the collected sperm for 30-60 minutes at 37⁰ C is to allow for capacitation, making them fertile.
Laparoscopic Egg Retrieval
- Laparoscopy may be used to retrieve eggs in the IVF process in certain circumstances.
Role of Human Chorionic Gonadotropin (hCG)
- Human chorionic gonadotropin (hCG) triggers ovulation, causing the eggs to mature and be released from the ovaries.
Ovarian Stimulation Monitoring
- Regular monitoring by ultrasound scan during ovarian stimulation is necessary to track follicular growth and prevent ovarian hyperstimulation syndrome (OHSS).
Test your knowledge about the process of in vitro fertilization (IVF), which involves fertilizing an egg by sperm outside the body. Explore the history, uses, and significance of IVF in addressing infertility and genetic issues.
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