The Role of Blood in Forensics and Biology
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The Role of Blood in Forensics and Biology

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Questions and Answers

What is the correct method for storing samples of blood?

  • Seal in a plastic bag and expose to sunlight.
  • Freeze the samples immediately.
  • Package in an envelope and store dry and cool. (correct)
  • Store in a warm environment to prevent degradation.
  • What is the first step in collecting a water sample?

  • Collect water with a clean pipette or syringe. (correct)
  • Mix the water with another chemical first.
  • Use a plastic syringe to inject air.
  • Pour the water directly into an envelope.
  • When dealing with dry blood, how should one prepare the swab for collection?

  • Soak the swab in hot water before use.
  • Cut the swab to expose more surface area.
  • Leave the swab dry to avoid contamination.
  • Moisten a swab with sterile/distilled water. (correct)
  • What is a critical question when assessing the nature of a blood stain?

    <p>Does the stain contain blood or another substance?</p> Signup and view all the answers

    In case of a blood sample suspected to be human, what additional investigation must be performed?

    <p>Identify if the blood is that of the victim, the accused, or others.</p> Signup and view all the answers

    What can the presence of blood at a crime scene indicate about the victim's status?

    <p>The victim may have survived the initial attack.</p> Signup and view all the answers

    Which type of blood is bright red and indicates oxygenation?

    <p>Arterial Blood</p> Signup and view all the answers

    How does blood contribute to the regulation of body temperature?

    <p>By circulating between body regions to equilibrate temperature.</p> Signup and view all the answers

    Which characteristic distinguishes venous blood from arterial blood?

    <p>Venous blood appears dark red due to increased carbon dioxide levels.</p> Signup and view all the answers

    What indicates that a crime may have been committed only a few hours to days prior?

    <p>Brownish blood with clots.</p> Signup and view all the answers

    What is one of the primary functions of blood?

    <p>To regulate internal body temperature.</p> Signup and view all the answers

    What is indicated by the color and consistency changes in blood at a crime scene?

    <p>The length of time since the blood was shed.</p> Signup and view all the answers

    What can lead to metabolic acidosis and potentially affect blood pH levels?

    <p>Excess carbon dioxide accumulation.</p> Signup and view all the answers

    What is the viscosity of blood compared to water?

    <p>4-5 times more viscous than water</p> Signup and view all the answers

    What is the specific gravity of blood?

    <p>1.065</p> Signup and view all the answers

    What is the normal pH range of blood?

    <p>7.35 - 7.45</p> Signup and view all the answers

    Which type of blood cell is primarily responsible for oxygen transport?

    <p>Red Blood Cells</p> Signup and view all the answers

    What is the normal range for white blood cells in mm3?

    <p>5,000 - 10,000</p> Signup and view all the answers

    What is the function of platelets in the blood?

    <p>Blood coagulation</p> Signup and view all the answers

    What is the liquid portion of blood called?

    <p>Plasma</p> Signup and view all the answers

    Which of the following is NOT a component of the formed elements in blood?

    <p>Nutrients</p> Signup and view all the answers

    Study Notes

    Importance of Blood Study

    • Blood can be used as evidence in legal cases.
    • Blood analysis can help determine disputed parentage in custody cases.
    • Blood can be used for DNA typing instead of buccal swabs.
    • Blood can help determine the time of death and how long the victim survived the attack.
      • A pool of blood indicates the victim may have been alive after the attack.
      • No pool of blood suggests immediate death.
    • Blood spatter analysis can determine the direction of escape for the victim or assailant.
      • Circular blood spatter indicates dripping blood.
      • Angled blood spatter indicates blood being projected.

    Nature of Blood

    • Blood is the largest circulating tissue in the body.
    • It has a fluid-like consistency due to its vital substances.
    • It contains blood cells, nutrients, and electrolytes.
    • It circulates within the cardiovascular system.
      • Transports oxygen, nutrients, and waste products.

    Functions of Blood

    • Transportation:
      • Gases (oxygen and carbon dioxide)
      • Nutrients
      • Waste products
    • Temperature Regulation
    • pH Regulation:
      • Maintains a slightly alkaline pH (7.35-7.45).
      • Metabolic acidosis can cause disease, such as arthritis.
    • Medication Transport:
      • Delivers medicine to affected parts of the body.

    Kinds of Blood

    • Arterial Blood (Capillary Blood):
      • Bright red in color due to oxygen transport.
      • Oxygenated blood.
    • Venous Blood:
      • Increased amount of CO2.
      • Dark red in color.
      • Non-oxygenated blood.

    Characteristics of Blood

    • Color:
      • Bright red (arterial blood)
      • Dark red (venous blood)
    • Volume: 70% of total body weight.
      • 80-85 mL/kg body weight.
    • Viscosity:
      • Resistance of blood to flow.
      • Thick and sticky.
      • 4-5 times more viscous than water.
    • Specific Gravity:
      • Blood = 1.065 due to cellular elements.
      • Water = 1.000.
    • pH Reaction:
      • Slightly alkaline (pH = 7.35-7.45).
      • Lower than 7 is acidic.
      • Higher than 7 is basic (alkaline).

    Composition of Blood

    • A. Formed Elements (35%)*

    • Red Blood Cells (Erythrocytes):

      • Number: 5,000,000 RBCs/mm3.
      • Shape: Biconcave disc.
      • Hemoglobin (Hb):
        • Coloring matter of blood.
        • Pigment found in the cytoplasm of RBCs.
        • Carries and transports oxygen.
    • White Blood Cells (Leukocytes):

      • Number: 5,000-10,000/mm3.
      • Soldiers of the Body.
        • Agranulocytes:
          • Monocytes
          • Lymphocytes
        • Granulocytes:
          • Eosinophils
          • Neutrophils
          • Basophils
    • Platelets (Thrombocytes):

      • Number: 150,000-350,000/mm3.
      • Function: Blood coagulation (blood clotting).
    • B. Liquid Portion (65% of total blood volume)*

    • Plasma: Straw liquid portion of the blood.

    • Serum: Straw-yellowish liquid when blood is allowed to clot

      • Used for testing such as drug analysis.

    Collection, Preservation, Packing, and Transportation of Blood Specimens

    • Blood on Removable Materials:
      • Remove the whole object.
      • Pack in a paper bag or envelope, keep dry and cool.
    • Pools of Blood:
      • Collect samples by swabbing from various locations.
      • Pack in an envelope, store dry and cool.
    • Blood in Water:
      • Collect water with a clean pipette or syringe.
      • Pour into a clean, dry test tube or vacuum tube with a purple cork.
    • Dry Blood:
      • Moisten a swab with sterile/distilled water.
      • Rub the swab on the stain until it becomes brown/red or absorbs all the blood.
      • Pack in a swab wrapper or folded paper, store dry and cool.

    ### Problems in the Study of Blood

    • Locating Blood:
      • Determining where to search for blood.
    • Specimen Collection:
      • Proper preservation and transportation of blood specimens.
    • Blood Stain Identification:
      • Determining if the stain is blood.
    • Blood Source Identification:
      • Determining if the blood is human or animal.
    • Blood Origin Identification:
      • Determining if the blood came from the victim, accused, or other persons.

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