14 Questions
What is the purpose of multiplex PCR?
To target multiple regions with multiple pairs of primers
Which of the following is true about the majority of diseases?
They are caused by point mutations in less than 25% of cases.
What are short tandem repeats (STRs)?
Highly selective and polymorphic sequences of DNA between genes
What is the function of CRISPR-Cas9?
It is an adaptive immune system of Streptococcus pyogenes against bacteriophages.
What is the function of the AMEL gene in the y chromosome?
To be shorter due to deletion
Which repair mechanism is more accurate?
Homology-directed repair (HDR
What is an electropherogram used for in DNA profiling?
To match peaks in DNA profiling
What is PAM in CRISPR-Cas9?
A target where CRISPR-Cas9 cuts.
What is cDNA?
Sequences of DNA that are transcribed from exonic regions
What can double-strand breaks lead to?
Both NHEJ and HDR.
What is the function of plasmids in bacteria?
To grow in bacteria autonomously
What is the accuracy of base editors for single point mutations?
Not accurate at all.
What is involved in the process of CRISPR-Cas9?
Guide RNA, Cas, nuclease, and double-stranded DNA
What is the process of producing human insulin in bacteria?
Bacteria produce insulin in a fermentation tank, followed by extraction and purification of human insulin
Study Notes
Cycle 10-1:
- PCR is used to make new DNA strands.
- Multiplex PCR targets multiple regions with multiple pairs of primers.
- Short tandem repeats (STRs) are sequences of DNA between genes that are highly selective and polymorphic.
- The AMEL gene in the y chromosome is shorter due to deletion.
- Electropherogram is used to match peaks in DNA profiling.
- cDNA only represents exonic sequences and is derived from mRNA.
- RT-PCR is used to determine mRNA levels or transcription levels.
- Plasmids are circular DNA that grow in bacteria autonomously.
- CDNA of human insulin is transcribed into mRNA protein and used to produce insulin in bacteria.
- Bacteria multiply and produce insulin in a fermentation tank, followed by extraction and purification of human insulin.
Cycle 10-2:
- CRISPR is the immune system of bacteria.
- CRISPR keeps memorized immune sequences in CRISPR locus.
Test your knowledge on molecular biology and genetics with our two cycles of short and informative statements. Cycle 10-1 covers topics such as PCR, STRs, electropherograms, plasmids, and more. Cycle 10-2 delves into the fascinating world of CRISPR, the bacterial immune system that has revolutionized gene editing. See how many keywords specific to each topic you can identify in this quiz.
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