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Questions and Answers
Which of the following is true about Eliza?
Which of the following is true about Eliza?
- Eliza is an immunoassay
- Eliza is a blood typing test
- Eliza is a serological assay
- Eliza is an enzyme linked Immunosorbent assay (correct)
What is the purpose of immunoassays?
What is the purpose of immunoassays?
- To interact with antigens
- To detect and quantify analytes (correct)
- To assess the presence of drugs or hormones
- To perform blood typing
What is the specific combination utilized in immunoassays?
What is the specific combination utilized in immunoassays?
- Antigen and antibody (correct)
- Antibody and analyte
- Enzyme and analyte
- Antibody and enzyme
What is the term used for the action of antibody clumping together?
What is the term used for the action of antibody clumping together?
Which antibody is used as the primary antibody in this experiment?
Which antibody is used as the primary antibody in this experiment?
What is the purpose of the secondary antibody in this experiment?
What is the purpose of the secondary antibody in this experiment?
What instrument is used to measure the level of color in each well?
What instrument is used to measure the level of color in each well?
What is the purpose of the calibration curve in this experiment?
What is the purpose of the calibration curve in this experiment?
What is the purpose of the X line in a lateral flow test for pregnancy?
What is the purpose of the X line in a lateral flow test for pregnancy?
What is the sensitivity of the lateral flow test for COVID when performed by trained lab scientists?
What is the sensitivity of the lateral flow test for COVID when performed by trained lab scientists?
What kind of result do we call it if the test fails to detect a positive result when the individual is actually positive?
What kind of result do we call it if the test fails to detect a positive result when the individual is actually positive?
What do the two lines in a lateral flow test represent?
What do the two lines in a lateral flow test represent?
Which of the following is the purpose of blocking with milk in the assay?
Which of the following is the purpose of blocking with milk in the assay?
What is the purpose of adding a calibration curve in the assay?
What is the purpose of adding a calibration curve in the assay?
What is the role of the secondary antibody conjugated to an enzyme in the assay?
What is the role of the secondary antibody conjugated to an enzyme in the assay?
What is the purpose of washing during the assay steps?
What is the purpose of washing during the assay steps?
What is the purpose of the indirect Eliza format?
What is the purpose of the indirect Eliza format?
What is the basis of the sandwich format in Eliza?
What is the basis of the sandwich format in Eliza?
When would a competitive Eliza format be utilized?
When would a competitive Eliza format be utilized?
What is the purpose of a lateral flow immunoassay?
What is the purpose of a lateral flow immunoassay?
What is the purpose of the enzyme in the enzyme-linked immunosorbent assay (ELISA)?
What is the purpose of the enzyme in the enzyme-linked immunosorbent assay (ELISA)?
What is the first step in performing an indirect ELISA?
What is the first step in performing an indirect ELISA?
What is the purpose of the secondary antibody in an indirect ELISA?
What is the purpose of the secondary antibody in an indirect ELISA?
What is the main function of the ELISA assay analyser?
What is the main function of the ELISA assay analyser?
Which of the following best describes the purpose of setting up triplicate samples in this experiment?
Which of the following best describes the purpose of setting up triplicate samples in this experiment?
What does the term 'background' refer to in this experiment?
What does the term 'background' refer to in this experiment?
What is the purpose of the calibration curve in this experiment?
What is the purpose of the calibration curve in this experiment?
What is the unit used to measure the antibody concentration in this experiment?
What is the unit used to measure the antibody concentration in this experiment?
Why are triplicate samples used for the patient samples in this experiment?
Why are triplicate samples used for the patient samples in this experiment?
What is the purpose of calculating the standard deviation in this experiment?
What is the purpose of calculating the standard deviation in this experiment?
Which of the following is true about live-attenuated vaccines?
Which of the following is true about live-attenuated vaccines?
What is the primary infection caused by Varicella-Zoster virus?
What is the primary infection caused by Varicella-Zoster virus?
What is the main purpose of the Varicella-Zoster vaccine?
What is the main purpose of the Varicella-Zoster vaccine?
Why is the Varicella-Zoster vaccine not on the UK schedule at present?
Why is the Varicella-Zoster vaccine not on the UK schedule at present?
What is the purpose of adjuvants in vaccines?
What is the purpose of adjuvants in vaccines?
What is the classification of active vaccines based on the antigen used?
What is the classification of active vaccines based on the antigen used?
Which of the following is a drawback of subunit vaccines?
Which of the following is a drawback of subunit vaccines?
What is the main challenge associated with mRNA vaccines?
What is the main challenge associated with mRNA vaccines?
What is the advantage of viral vector vaccines?
What is the advantage of viral vector vaccines?
What is the role of adjuvants in vaccination?
What is the role of adjuvants in vaccination?
What is the purpose of vaccine conjugation?
What is the purpose of vaccine conjugation?
What is the main advantage of lenteviral vaccines?
What is the main advantage of lenteviral vaccines?
Which organism is responsible for the production of the BCG vaccine?
Which organism is responsible for the production of the BCG vaccine?
What is the main aim of the TB vaccination?
What is the main aim of the TB vaccination?
What is the effectiveness of the BCG vaccine in preventing disseminated TB and TB meningitis in children?
What is the effectiveness of the BCG vaccine in preventing disseminated TB and TB meningitis in children?
Which type of vaccines generally require boosters to maintain immunity?
Which type of vaccines generally require boosters to maintain immunity?
Which antigenic components are targeted by protective antibody responses in influenza vaccines?
Which antigenic components are targeted by protective antibody responses in influenza vaccines?
What is the purpose of adjuvants in subunit vaccines?
What is the purpose of adjuvants in subunit vaccines?
Which of the following is an example of passive immunisation?
Which of the following is an example of passive immunisation?
What is the term used for the process by which an individual is rendered immune through artificial means?
What is the term used for the process by which an individual is rendered immune through artificial means?
Which of the following vaccine types is NOT mentioned in the text?
Which of the following vaccine types is NOT mentioned in the text?
What is the historical background of immunisation mentioned in the text?
What is the historical background of immunisation mentioned in the text?
What is the estimated reduction in mortality worldwide due to immunisation?
What is the estimated reduction in mortality worldwide due to immunisation?
What is the purpose of adjuvants and vaccine conjugation in vaccination?
What is the purpose of adjuvants and vaccine conjugation in vaccination?
Which of the following is true about the varicella-zoster virus (VZV) vaccination?
Which of the following is true about the varicella-zoster virus (VZV) vaccination?
What is the difference between the Salk and Sabin polio vaccines?
What is the difference between the Salk and Sabin polio vaccines?
What is the main function of macrophages during primary tuberculosis infection?
What is the main function of macrophages during primary tuberculosis infection?
What is the purpose of the zoster vaccination?
What is the purpose of the zoster vaccination?
What is the route of spread of the polio virus during the viremia phase?
What is the route of spread of the polio virus during the viremia phase?
What is the incidence of zoster in parallel with?
What is the incidence of zoster in parallel with?
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