Test Your DNA Replication Know-How

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Questions and Answers

What is the function of helicase in DNA replication?

  • To protect the DNA from nucleases
  • To bind to the replication fork and break the hydrogen bonds between the DNA strands (correct)
  • To supercoil the DNA
  • To prevent the DNA strands from reforming a double helix

What is the function of single stranded binding proteins (SSB) in DNA replication?

  • To break the hydrogen bonds between the DNA strands
  • To protect the DNA from nucleases
  • To keep both the strands of DNA separated and prevent them from forming a double helix again (correct)
  • To supercoil the DNA

What is the energy source used by helicase in DNA replication?

  • GTP
  • ADP
  • CTP
  • ATP (correct)

Why is supercoiled DNA unwound by DNA topoisomerases during DNA replication?

<p>Supercoiled DNA ahead of replication fork will interfere with helicase in strand separation (C)</p> Signup and view all the answers

What is the function of DNA topoisomerase I in DNA replication?

<p>To break only one strand of DNA (A)</p> Signup and view all the answers

What is the function of DNA topoisomerase II in DNA replication?

<p>To break both strands of DNA (A)</p> Signup and view all the answers

What is the consequence of supercoiled DNA ahead of the replication fork?

<p>It interferes with helicase in strand separation (C)</p> Signup and view all the answers

What is the function of ATP in DNA replication?

<p>To provide energy for helicase to break the hydrogen bonds between the DNA strands (B)</p> Signup and view all the answers

What is the function of SSB in protecting the DNA during replication?

<p>To prevent the DNA from reforming a double helix and protect the DNA from nucleases (A)</p> Signup and view all the answers

What is the consequence of supercoiled DNA during DNA replication?

<p>It needs to be unwound by DNA topoisomerases (D)</p> Signup and view all the answers

What is the function of DNA topoisomerases in DNA replication?

<p>To unwind supercoiled DNA by cutting and resealing the DNA strands (C)</p> Signup and view all the answers

What is the function of SSB in preventing the DNA from being degraded?

<p>To protect the DNA from nucleases that might degrade single stranded DNA (A)</p> Signup and view all the answers

What is the function of helicase in DNA replication?

<p>To bind to the replication fork and break the hydrogen bonds between the DNA strands (B)</p> Signup and view all the answers

What is the function of single stranded binding proteins (SSB) in DNA replication?

<p>To keep both the strands of DNA separated and prevent them from forming a double helix again (C)</p> Signup and view all the answers

What is the energy source used by helicase in DNA replication?

<p>ATP (A)</p> Signup and view all the answers

Why is supercoiled DNA unwound by DNA topoisomerases during DNA replication?

<p>Supercoiled DNA ahead of replication fork will interfere with helicase in strand separation (C)</p> Signup and view all the answers

What is the function of DNA topoisomerase I in DNA replication?

<p>To break only one strand of DNA (C)</p> Signup and view all the answers

What is the function of DNA topoisomerase II in DNA replication?

<p>To break both strands of DNA (D)</p> Signup and view all the answers

What is the consequence of supercoiled DNA ahead of the replication fork?

<p>It interferes with helicase in strand separation (A)</p> Signup and view all the answers

What is the function of ATP in DNA replication?

<p>To provide energy for helicase to break the hydrogen bonds between the DNA strands (A)</p> Signup and view all the answers

What is the function of SSB in protecting the DNA during replication?

<p>To prevent the DNA from reforming a double helix and protect the DNA from nucleases (B)</p> Signup and view all the answers

What is the consequence of supercoiled DNA during DNA replication?

<p>It needs to be unwound by DNA topoisomerases (D)</p> Signup and view all the answers

What is the function of DNA topoisomerases in DNA replication?

<p>To unwind supercoiled DNA by cutting and resealing the DNA strands (C)</p> Signup and view all the answers

What is the function of SSB in preventing the DNA from being degraded?

<p>To protect the DNA from nucleases that might degrade single stranded DNA (C)</p> Signup and view all the answers

What is the function of helicase in DNA replication?

<p>It binds to the replication fork and breaks the hydrogen bonds between the strands (C)</p> Signup and view all the answers

What is the function of single stranded binding proteins (SSB) in DNA replication?

<p>It helps to keep both the strands of DNA separated (C)</p> Signup and view all the answers

What is the function of DNA topoisomerases in DNA replication?

<p>To unwind supercoiled DNA ahead of the replication fork (D)</p> Signup and view all the answers

Which type of topoisomerase breaks only one strand of DNA?

<p>Topoisomerase I (A)</p> Signup and view all the answers

Which type of topoisomerase breaks both strands of DNA?

<p>Topoisomerase II (C)</p> Signup and view all the answers

What is the role of ATP in DNA replication?

<p>It provides energy for helicase to break the hydrogen bonds between the strands (C)</p> Signup and view all the answers

What happens to the double helix DNA during DNA replication?

<p>It is separated into single strand DNA by DnaA followed by Helicase and SSB (B)</p> Signup and view all the answers

What happens to supercoiled DNA ahead of the replication fork during DNA replication?

<p>It is unwound by DNA topoisomerases (A)</p> Signup and view all the answers

What is the consequence of supercoiled DNA ahead of the replication fork not being unwound during DNA replication?

<p>It will interfere with helicase in strand separation (A)</p> Signup and view all the answers

What is the consequence of DNA topoisomerase not resealing the cut made during DNA replication?

<p>It can lead to DNA damage and mutations (B)</p> Signup and view all the answers

What is the source of energy used by helicase in DNA replication?

<p>ATP (B)</p> Signup and view all the answers

What is the function of single stranded DNA in DNA replication?

<p>It serves as template for DNA polymerase to synthesize new DNA strands (A)</p> Signup and view all the answers

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