Test Your DNA Replication Know-How
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Questions and Answers

What is the function of helicase in DNA replication?

  • To protect the DNA from nucleases
  • To bind to the replication fork and break the hydrogen bonds between the DNA strands (correct)
  • To supercoil the DNA
  • To prevent the DNA strands from reforming a double helix

    • What is the function of single stranded binding proteins (SSB) in DNA replication?

  • To break the hydrogen bonds between the DNA strands
  • To protect the DNA from nucleases
  • To keep both the strands of DNA separated and prevent them from forming a double helix again (correct)
  • To supercoil the DNA

    • What is the energy source used by helicase in DNA replication?

  • GTP
  • ADP
  • CTP
  • ATP (correct)

    • Why is supercoiled DNA unwound by DNA topoisomerases during DNA replication?

    <p>Supercoiled DNA ahead of replication fork will interfere with helicase in strand separation</p> Signup and view all the answers

    What is the function of DNA topoisomerase I in DNA replication?

    <p>To break only one strand of DNA</p> Signup and view all the answers

    What is the function of DNA topoisomerase II in DNA replication?

    <p>To break both strands of DNA</p> Signup and view all the answers

    What is the consequence of supercoiled DNA ahead of the replication fork?

    <p>It interferes with helicase in strand separation</p> Signup and view all the answers

    What is the function of ATP in DNA replication?

    <p>To provide energy for helicase to break the hydrogen bonds between the DNA strands</p> Signup and view all the answers

    What is the function of SSB in protecting the DNA during replication?

    <p>To prevent the DNA from reforming a double helix and protect the DNA from nucleases</p> Signup and view all the answers

    What is the consequence of supercoiled DNA during DNA replication?

    <p>It needs to be unwound by DNA topoisomerases</p> Signup and view all the answers

    What is the function of DNA topoisomerases in DNA replication?

    <p>To unwind supercoiled DNA by cutting and resealing the DNA strands</p> Signup and view all the answers

    What is the function of SSB in preventing the DNA from being degraded?

    <p>To protect the DNA from nucleases that might degrade single stranded DNA</p> Signup and view all the answers

    What is the function of helicase in DNA replication?

    <p>To bind to the replication fork and break the hydrogen bonds between the DNA strands</p> Signup and view all the answers

    What is the function of single stranded binding proteins (SSB) in DNA replication?

    <p>To keep both the strands of DNA separated and prevent them from forming a double helix again</p> Signup and view all the answers

    What is the energy source used by helicase in DNA replication?

    <p>ATP</p> Signup and view all the answers

    Why is supercoiled DNA unwound by DNA topoisomerases during DNA replication?

    <p>Supercoiled DNA ahead of replication fork will interfere with helicase in strand separation</p> Signup and view all the answers

    What is the function of DNA topoisomerase I in DNA replication?

    <p>To break only one strand of DNA</p> Signup and view all the answers

    What is the function of DNA topoisomerase II in DNA replication?

    <p>To break both strands of DNA</p> Signup and view all the answers

    What is the consequence of supercoiled DNA ahead of the replication fork?

    <p>It interferes with helicase in strand separation</p> Signup and view all the answers

    What is the function of ATP in DNA replication?

    <p>To provide energy for helicase to break the hydrogen bonds between the DNA strands</p> Signup and view all the answers

    What is the function of SSB in protecting the DNA during replication?

    <p>To prevent the DNA from reforming a double helix and protect the DNA from nucleases</p> Signup and view all the answers

    What is the consequence of supercoiled DNA during DNA replication?

    <p>It needs to be unwound by DNA topoisomerases</p> Signup and view all the answers

    What is the function of DNA topoisomerases in DNA replication?

    <p>To unwind supercoiled DNA by cutting and resealing the DNA strands</p> Signup and view all the answers

    What is the function of SSB in preventing the DNA from being degraded?

    <p>To protect the DNA from nucleases that might degrade single stranded DNA</p> Signup and view all the answers

    What is the function of helicase in DNA replication?

    <p>It binds to the replication fork and breaks the hydrogen bonds between the strands</p> Signup and view all the answers

    What is the function of single stranded binding proteins (SSB) in DNA replication?

    <p>It helps to keep both the strands of DNA separated</p> Signup and view all the answers

    What is the function of DNA topoisomerases in DNA replication?

    <p>To unwind supercoiled DNA ahead of the replication fork</p> Signup and view all the answers

    Which type of topoisomerase breaks only one strand of DNA?

    <p>Topoisomerase I</p> Signup and view all the answers

    Which type of topoisomerase breaks both strands of DNA?

    <p>Topoisomerase II</p> Signup and view all the answers

    What is the role of ATP in DNA replication?

    <p>It provides energy for helicase to break the hydrogen bonds between the strands</p> Signup and view all the answers

    What happens to the double helix DNA during DNA replication?

    <p>It is separated into single strand DNA by DnaA followed by Helicase and SSB</p> Signup and view all the answers

    What happens to supercoiled DNA ahead of the replication fork during DNA replication?

    <p>It is unwound by DNA topoisomerases</p> Signup and view all the answers

    What is the consequence of supercoiled DNA ahead of the replication fork not being unwound during DNA replication?

    <p>It will interfere with helicase in strand separation</p> Signup and view all the answers

    What is the consequence of DNA topoisomerase not resealing the cut made during DNA replication?

    <p>It can lead to DNA damage and mutations</p> Signup and view all the answers

    What is the source of energy used by helicase in DNA replication?

    <p>ATP</p> Signup and view all the answers

    What is the function of single stranded DNA in DNA replication?

    <p>It serves as template for DNA polymerase to synthesize new DNA strands</p> Signup and view all the answers

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