Podcast
Questions and Answers
Which type of chromatography uses a supercritical fluid as the mobile phase?
Which type of chromatography uses a supercritical fluid as the mobile phase?
- Gas chromatography (GC)
- Supercritical fluid chromatography (SFC) (correct)
- High performance liquid chromatography (HPLC)
- Thin layer chromatography (TLC)
What type of molecules can be analyzed and purified using SFC?
What type of molecules can be analyzed and purified using SFC?
- Chiral compounds (correct)
- Thermally stable molecules
- Aromatic compounds
- High molecular weight molecules
What is another name for supercritical fluid chromatography?
What is another name for supercritical fluid chromatography?
- Reversed phase chromatography
- Convergence chromatography (correct)
- Normal phase chromatography
- Ion exchange chromatography
What is the primary application of SFC in the industry?
What is the primary application of SFC in the industry?
What must be done to the entire chromatographic flow path in SFC?
What must be done to the entire chromatographic flow path in SFC?
What are the principles of supercritical fluid chromatography?
What are the principles of supercritical fluid chromatography?
What is the primary use of SFC in the pharmaceutical industry?
What is the primary use of SFC in the pharmaceutical industry?
What type of molecules are commonly separated using SFC?
What type of molecules are commonly separated using SFC?
What is the term used to describe the state in which liquid and gas properties converge in SFC?
What is the term used to describe the state in which liquid and gas properties converge in SFC?
What type of columns are used in SFC?
What type of columns are used in SFC?
Study Notes
Supercritical Fluid Chromatography (SFC)
- Uses a supercritical fluid as the mobile phase.
Analyzing Molecules
- SFC is used to analyze and purify non-polar to moderately polar molecules.
- These molecules are often thermally labile, chiral, or have low volatility.
Alternative Names
- SFC is also known as High-Pressure Chromatography.
Industrial Applications
- The primary application of SFC in the industry is for purification and analysis of molecules.
- This is especially true for the pharmaceutical industry.
Instrumentation
- The entire chromatographic flow path must be pressurized in SFC.
Principles
- SFC operates based on the principles of adsorption chromatography.
- Separation occurs due to differences in the affinity of analytes for the stationary phase.
Pharmaceutical Applications
- The primary use of SFC in the pharmaceutical industry is for chiral separation and purity analysis.
Types of Molecules
- SFC is commonly used to separate chiral molecules, complex natural products, and polymeric molecules.
Supercritical State
- The supercritical state is a condition where liquid and gas properties converge.
- This state is characterized by high density and solubility, and low viscosity and surface tension.
Column Selection
- SFC columns are typically packed with stationary phases like silica or immobilized polysaccharide.
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Description
Test your knowledge of Supercritical Fluid Chromatography (SFC) principles and applications with this informative quiz. Explore the use of supercritical fluids as mobile phases and their advantages in analyzing and purifying thermally labile molecules. Dive into the separation of chiral compounds and compare SFC with high performance liquid chromatography.
