10 Questions
Which type of chromatography uses a supercritical fluid as the mobile phase?
Supercritical fluid chromatography (SFC)
What type of molecules can be analyzed and purified using SFC?
Chiral compounds
What is another name for supercritical fluid chromatography?
Convergence chromatography
What is the primary application of SFC in the industry?
Separation of chiral molecules
What must be done to the entire chromatographic flow path in SFC?
It must be pressurized
What are the principles of supercritical fluid chromatography?
The principles of supercritical fluid chromatography are similar to those of high performance liquid chromatography, but SFC typically utilizes carbon dioxide as the mobile phase.
What is the primary use of SFC in the pharmaceutical industry?
SFC is commonly used for achiral separations and purifications in the pharmaceutical industry.
What type of molecules are commonly separated using SFC?
SFC is primarily used for the separation of chiral compounds.
What is the term used to describe the state in which liquid and gas properties converge in SFC?
Supercritical fluid chromatography is sometimes called convergence chromatography.
What type of columns are used in SFC?
SFC uses the same columns as standard HPLC systems.
Study Notes
Supercritical Fluid Chromatography (SFC)
- Uses a supercritical fluid as the mobile phase.
Analyzing Molecules
- SFC is used to analyze and purify non-polar to moderately polar molecules.
- These molecules are often thermally labile, chiral, or have low volatility.
Alternative Names
- SFC is also known as High-Pressure Chromatography.
Industrial Applications
- The primary application of SFC in the industry is for purification and analysis of molecules.
- This is especially true for the pharmaceutical industry.
Instrumentation
- The entire chromatographic flow path must be pressurized in SFC.
Principles
- SFC operates based on the principles of adsorption chromatography.
- Separation occurs due to differences in the affinity of analytes for the stationary phase.
Pharmaceutical Applications
- The primary use of SFC in the pharmaceutical industry is for chiral separation and purity analysis.
Types of Molecules
- SFC is commonly used to separate chiral molecules, complex natural products, and polymeric molecules.
Supercritical State
- The supercritical state is a condition where liquid and gas properties converge.
- This state is characterized by high density and solubility, and low viscosity and surface tension.
Column Selection
- SFC columns are typically packed with stationary phases like silica or immobilized polysaccharide.
Test your knowledge of Supercritical Fluid Chromatography (SFC) principles and applications with this informative quiz. Explore the use of supercritical fluids as mobile phases and their advantages in analyzing and purifying thermally labile molecules. Dive into the separation of chiral compounds and compare SFC with high performance liquid chromatography.
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