Staines in Histologia

Podcast Beta

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Qual es le function principal de le colorante Giemsa?

Supportar le crescita de bacterias fastidios

Precipitar acidos nucleic

Etchari le pH de solutiones

Differentiari tipos de cellulas in hematologia (correct)

Como le acido acetic contribui durante le extraction de DNA?

Elimina le contamination de bacterias

Facilita le precipitation de acidos nucleic (correct)

Colora le nucleos de cellulas

Inhibi le crescita de organismos fastidios

Qual description corresponde a le agar nutrient?

Un medium pro extraction de DNA

Un medium general pro bacterias non-fastidios (correct)

Un medium special pro bacterias fastidios

Un medium usate pro colorar cellulas

Quo es uno de le usages del colorante Giemsa?

Stainar cellulas sanguinee e parasitas Signup and view all the answers

Qual de le affirmations super le acido acetic es ver?

Conveniente pro adjustment de pH e extraction de DNA Signup and view all the answers

Quale color resulta del Gram Stain pro bacteria Gram-positive?

Viola Signup and view all the answers

Quale color es usate in le Periodic Acid-Schiff (PAS) Stain pro indicar glycogen?

Magenta Signup and view all the answers

C quale staine es usate pro detecter Mycobacterium tuberculosis?

Ziehl-Neelsen Stain Signup and view all the answers

Quale es le principale uso del Giemsa Stain?

Examinatio de cellulas sanguinee Signup and view all the answers

C quale staine es usate pro visualization de lipidos?

Sudan Stain Signup and view all the answers

Quale color indica la presence de fibras reticulares in le Silver Stain?

Argento Signup and view all the answers

Quale color es associate con le Alcian Blue Stain pro acidic mucopolysaccharides?

Blu Signup and view all the answers

C quale staine es preferibile pro differentiating blood cell types?

Wright's Stain Signup and view all the answers

Quale color es tipicamente observate in le amyloid deposits sub microscopia?

Verde appello Signup and view all the answers

Quale staine identifica capsules in microorganismos?

India Ink Signup and view all the answers

Quale reagens es usate pro detectar saccharos reductores?

Reagens de Benedict Signup and view all the answers

Quale reagens deveni violet quando peptide bonds es presente?

Reagens de Biuret Signup and view all the answers

Quale es le function de Sodium Hydroxide in biologia?

Neutralisation de pH Signup and view all the answers

Quale reagens es usate pro la fixation de cellules in smeares de sang?

Formalin Signup and view all the answers

Quale reagens es usate in titrationes pro ajustar pH?

Sodium Hydroxide Signup and view all the answers

Quale reagens es usate pro precipitar DNA/RNA?

Ethanol Signup and view all the answers

Quale reagens es un indicatore de pH in titrationes?

Phenolphthalein Signup and view all the answers

Quale reagens es usate pro la decoloration de bacteria in histologia?

Iodino de Gram Signup and view all the answers

Quale es le principal uso de Acido Clorhidric in biologia?

Digestion de samples biologicos Signup and view all the answers

Quale reagens es usate pro la salting out de protenas?

Ammonium Sulfate Signup and view all the answers

Qual tipo de agar es specificate per la detection de helomolysis?

Blood Agar Signup and view all the answers

Qual agar es preparate con un pH acide e un concentration alt de sugar pro inhiber le crescimento bacteric?

Sabouraud Dextrose Agar Signup and view all the answers

Qual agar es selective pro bacterias Gram-negative e permitte la differentiation de fermentatores de lactose?

MacConkey Agar Signup and view all the answers

Qual agar es specificate pro l'isolamento de Neisseria gonorrhoeae?

Thayer-Martin Agar Signup and view all the answers

Qual de le sequente agar es usate pro cultivar bacterias anaerobias?

Thioglycolate Broth Signup and view all the answers

Qual agar permite le identificatión de Salmonella e Shigella?

Xylose Lysine Deoxycholate Agar Signup and view all the answers

Qual agar es enrichite pro permetter le crecimiento de organism fastidios?

Blood Agar Signup and view all the answers

Qual agar es selective pro Pseudomonas aeruginosa?

Cetrimide Agar Signup and view all the answers

Qual agar permite le differentiation de fermentatores de mannitol pro Staphylococcus?

Mannitol Salt Agar Signup and view all the answers

Qual de le sequente agares es usate pro la cultura de Legionella species?

Buffered Charcoal Yeast Extract Signup and view all the answers

Study Notes

Staines e su usos

Hematoxylin e Eosin (H&E): Un stain general usate in histologia pro colorar nucleos (hematoxylin, color blau) e cytoplasma (eosin, color rosate).
Gram Stain: Differentiate le parietes cellular de bacterias in duo gruppo: gram-positive (color purpur) e gram-negative (color rose).
Ziehl-Neelsen Stain: Detecta bacterias acido-resistente, como Mycobacterium tuberculosis (color rubie).
Giemsa Stain: Examination de cellulas sanguinee e detection de parasitos; colorate cellulas sanguinee e parasitos (p.ex., Plasmodium in malaria).
Periodic Acid-Schiff (PAS) Stain: Detecta glycogen e mucopolysaccharidos; colorate glycogen e altere carbohydratos (color magenta).
Wright's Stain: Examination de smears de sanguine; differentia le typos de cellulas sanguinee.
Silver Stain: Visualisation de fibras reticulares e fungos; colorate fibras reticulares, certe bacterias e fungos.
Trichrome Stain: Differentia musculo, collageno e cytoplasma; colorate fibras muscular (color rubie), collageno (color blau/verde) e cytoplasma (color rose).
Sudan Stain: Colorate lipidos; colorate lipidos (color orange o nigre dependente del typo).
Alcian Blue: Colorate mucopolysaccharidos acide; colorate mucopolysaccharidos acide (color blau).
India Ink: Visualisation de capsulas in microorganismos; colorate capsulas, particularmente in Cryptococcus neoformans.
Congo Red: Detection de amyloide in tissus; colorate depositos de amyloide (color rubie sub le microscopio optic; birefringentia verde-pomme sub lumine polarisate).

Reagentes e su usos

Benedict's Reagent: Detection de sucres reductante; cambia color de blau a orange/rubie in presentia de sucres reductante.
Biuret Reagent: Detection de proteines; diventa violette in presentia de ligationes peptidic in proteines.
Fehling's Solution: Detecta sucres reductante, specialmente in carbohydratos; cambia de blau a un precipitate rubie-brique si le sucres reductante es presente.
Gram's Iodine: Usate in le coloration de Gram pro bacterias; agisce como un mordante pro fixar le stain de violetto de crystall in bacterias gram-positive.
Sodium Hydroxide (NaOH): Regulation del pH; reactiones de neutralisation; un base forte communmente usate in titrationes e pro ajustar le pH.
Hydrochloric Acid (HCl): Regulation del pH; usate in le digestion de specimens biologic; un acido forte usate in titrationes e pro dissolver specimens.
Ethanol: Usate in le purification de ADN/ARN e le coloration de Gram; precipita ADN/ARN e decolorise in le coloration de Gram.
Formalin: Fixation de tissus in histologia; conserva le tissus per ligação de proteines.
Methanol: Fixation in smears sanguinee e ADN; fixa cellulas e precipita acidonucleic.
Phenolphthalein: Indicator del pH in titrationes acido-base; deveni rose in solutiones basic.
Acetone: Usate como un agente de deshydratation e solvente; communmente usate in histologia pro deshydratation.
Silver Nitrate: Coloration in histologia e microbiologia; colorate bacterias e fungos; etiam usate in cura de plagas como un antiseptic.
Sodium Chloride (NaCl): Mantene le equilibro osmotic in cellulas e specimens de tissus; communmente usate in tampones physiologic.
Ammonium Sulfate: Precipitation de proteines; precipita proteines per "salting out".
Tris Buffer: Stabilisa le pH in solutiones biologic; un tampon commun pro mantener le pH.
Giemsa Stain: Colorate cellulas sanguinee e parasitos; usate in hematologia pro differentiar le typos de cellulas.
Acetic Acid: Usate in le extraction de ADN e le regulation del pH; aiuta in le precipitation de acidonucleic.

Typos de cultures e su usos

Nutrient Agar: Medium general-proposito pro bacterias non-fastidiose; supporta un ample gamma de crescentia bacterial.
Blood Agar: Detecta le activitate hemolytic de bacterias; arricchite con sanguine de oves o human; monstra patrones de hemolyse (alpha, beta, gamma).
Chocolate Agar: Cresce organismos fastidiose como Haemophilus e Neisseria; agar sanguinee calefacite; libera factores de crescentia como NAD e hemin.
MacConkey Agar: Selective pro bacterias Gram-negative; differencia fermentatores de lactose; contine sales de bile e violetto de crystall; fermentatores de lactose deveni rose.
Mannitol Salt Agar (MSA): Selective pro species de Staphylococcus; differencia fermentatores de mannitol; alte concentration de sal; fermentatores de mannitol deveni jalne.
Thayer-Martin Agar: Isolation de Neisseria gonorrhoeae e Neisseria meningitidis; contine antibioticos pro inhibir le flora normal.
Sabouraud Dextrose Agar: Cultura de fungos, specialmente levuras e moldes; pH acide e alte concentration de zucchero inhibi le crescentia bacterial.
Cetrimide Agar: Selective pro Pseudomonas aeruginosa; contine cetrimide, que inhibi altere bacterias.
Buffered Charcoal Yeast Extract (BCYE): Cultures Legionella species; arricchite con carbon pro detoxificar productes de decomposition que inhibi le crescentia.
Xylose Lysine Deoxycholate (XLD) Agar: Selective pro Salmonella e Shigella; Salmonella appare con centros nigre; colonias de Shigella es transparente.
Lowenstein-Jensen Medium: Isolation de Mycobacterium tuberculosis; contine verde de malachite pro inhibir altere bacterias.
Campy-BAP (Campylobacter Blood Agar): Isolation de species de Campylobacter; arricchite con antibioticos pro inhibir le flora intestinal normal.
Thioglycolate Broth: Cultivation de bacterias anaerobic; agentes reductante crea un gradiential de oxygeno pro anaerobes al fundo.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Este quiz explora diverse staines usate in histologia e microbiologia. Discute le differenti tipos de staines como H&E, Gram, e Ziehl-Neelsen, e lor applicationes. Testa tu cognoscentia super le colorations e le microorganismos que illos detecta.