Histology Stains and Markers Quiz
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Questions and Answers

Match the stain with its primary usage:

Prussian blue stain = Iron in hemosiderin turns blue to black when exposed to potassium ferrocyanide Thioflavin T = Used for amyloidosis Mucicarmine stain = Used for adenocarcinoma Periodic Acid Schiff (PAS) = Useful in identifying fungi, parasites, glycogen, and the basement membrane

Match the marker with its staining pattern:

CD34 = Positive in DFSP Factor XIIIa = Negative in DFSP S100 protein = Non-specific stain commonly used in the diagnosis of melanoma Neuron specific enolase = Stains merkel cells

Match the staining technique with its target substance:

Fontana Masson stain = Melanin black for dematiaceous fungi identification Gormorimethenamine silver = Stains fungi, parasites, Donovan bodies, and Rhinoscleroma black Von Kossa stain = Identifies calcium by staining it black Cytokeratin 7 = Associated with extramammary Paget's disease

Match the stain with its target organism or substance:

<p>Cryptococcus capsule stain = Stains Cryptococcus capsule red Rhinosporidium = Stained by Periodic Acid Schiff (PAS) HMB45 = Stains melanocytic lesions such as melanoma CD68 = Positive in Rosai Dorfman syndrome</p> Signup and view all the answers

Match the following immunohistochemical stains with their respective tumor types:

<p>S100 +ve, CD68 +ve = Atypical fibroxanthoma Vimentin +ve, Smooth muscle actin +ve = Glomus tumor Vimentin +ve, Smooth muscle actin +ve, Desmin +ve = Leiomyosarcoma S100 +ve, MBP +ve, Vimentin +ve = Schwannoma or Neurolemmoma</p> Signup and view all the answers

Match the following gene mutations with their associated conditions:

<p>MEFV gene = FMF: Pyrin SPG31 = Spastic paraplegia CARD15 (NOD2) gene = Early onset sarcoidosis CKIT = Mastocytosis</p> Signup and view all the answers

Match the following neoplasms with their characteristic immunohistochemical stains:

<p>Merkel cell carcinoma = S100 +ve, HMB45 +ve, Vimentin +ve Desmoplastic melanoma = Vimentin +ve, Smooth muscle actin +ve, Desmin +ve Trichoblastoma = Vimentin +ve, S100 +ve Basal cell carcinoma = CK20, Paget disease</p> Signup and view all the answers

Match the following syndromes with their associated gene mutations or tests:

<p>Carcinoid syndrome = Metastasis to the liver POEMS syndrome = Lambda light chain</p> Signup and view all the answers

Match the following skin lesions with their most likely locations on the body:

<p>Venous lake = Lower lip basal cell carcinoma = Scalp Trichoblastoma = Scalp syringocystadenoma papilliferum = Scalp</p> Signup and view all the answers

What is the most likely diagnosis based on the clinical and histologic findings of a 63-year-old female with a papillomatous yellow-orange plaque on the scalp and a smooth, pink nodule paracentrally within the plaque?

<p>Trichoblastoma arising in association with nevus sebaceus</p> Signup and view all the answers

In suspected carcinoid syndrome, which urine test should be ordered?

<p>5-HIAA</p> Signup and view all the answers

What is the most common cancer in POEMS syndrome?

<p>Osteosclerotic myeloma</p> Signup and view all the answers

Where on the body would a venous lake most likely present?

<p>Lower lip or the ear</p> Signup and view all the answers

What are the immunohistochemical markers for DFSP?

<p>CD+ve, vimentin +ve</p> Signup and view all the answers

What are the immunohistochemical markers for atypical fibroxanthoma?

<p>S100+ve, CD68+ve</p> Signup and view all the answers

What are the immunohistochemical markers for shwanomma or Neurolemmoma?

<p>S100+ve, MBP +ve, vimentin +ve</p> Signup and view all the answers

What are the immunohistochemical markers for melanoma?

<p>S100 +ve, HMB45+ve, vimentin +ve</p> Signup and view all the answers

What are the immunohistochemical markers for glomus tumor?

<p>Vimentin +ve, Smooth muscle actin +ve</p> Signup and view all the answers

What are the immunohistochemical markers for leimyosarcoma?

<p>Vimentin +ve, Smooth muscle actin +ve, Desmin +ve</p> Signup and view all the answers

What is the gene associated with mastocytosis?

<p>CKIT</p> Signup and view all the answers

What is the gene associated with early onset sarcoid?

<p>CARD15 (NOD2) gene</p> Signup and view all the answers

What stain is used to identify iron in hemosiderin?

<p>Perls' Prussian blue stain</p> Signup and view all the answers

Which stain is useful in identifying fungi, parasites, glycogen, and the basement membrane?

<p>Periodic Acid Schiff (PAS)</p> Signup and view all the answers

What stain is used to identify melanin and helps identify dematiaceous fungi?

<p>Fontana Masson stain</p> Signup and view all the answers

Which stain is used to identify calcium by staining it black?

<p>Von Kossa stain</p> Signup and view all the answers

What stain is commonly used as an adjunctive marker in the diagnosis of melanoma?

<p>S100 protein</p> Signup and view all the answers

Which stain is used to identify amyloidosis?

<p>Thioflavin T</p> Signup and view all the answers

What stain is used to identify extramammary Paget's disease?

<p>Cytokeratin 7</p> Signup and view all the answers

Which stain is used to identify adenocarcinoma?

<p>Mucicarmine stain</p> Signup and view all the answers

What stain is used to identify mastocytosis?

<p>Toulidine blue</p> Signup and view all the answers

Which stain stains fungi, parasites, Donovan bodies, and Rhinoscleroma black?

<p>Gormorimethenamine silver</p> Signup and view all the answers

What stain is used to identify merkel cells?

<p>Neuron specific enolase</p> Signup and view all the answers

Which stain is positive in Rosai Dorfman syndrome?

<p>CD68</p> Signup and view all the answers

Which stain is used to identify iron in hemosiderin?

<p>Perls' Prussian blue stain</p> Signup and view all the answers

What stain is commonly used as an adjunctive marker in the diagnosis of melanoma?

<p>S100 protein</p> Signup and view all the answers

What stain is used to identify amyloidosis?

<p>Thioflavin T</p> Signup and view all the answers

What stain is used to identify mastocytosis?

<p>Toulidine blue</p> Signup and view all the answers

What stain is used to identify melanin and helps identify dematiaceous fungi?

<p>Fontana Masson stain</p> Signup and view all the answers

What stain is used to identify calcium by staining it black?

<p>Von Kossa stain</p> Signup and view all the answers

What is the most likely diagnosis based on the clinical and histologic findings of a 63-year-old female with a papillomatous yellow-orange plaque on the scalp and a smooth, pink nodule paracentrally within the plaque?

<p>Trichoblastoma arising in association with nevus sebaceus</p> Signup and view all the answers

In suspected carcinoid syndrome, which urine test should be ordered?

<p>5-HIAA</p> Signup and view all the answers

What is the most common cancer in POEMS syndrome?

<p>Osteosclerotic myeloma</p> Signup and view all the answers

Where on the body would a venous lake most likely present?

<p>Lower lip or the ear</p> Signup and view all the answers

What gene mutation is associated with mastocytosis?

<p>CKIT</p> Signup and view all the answers

What stain is used to identify extramammary Paget's disease?

<p>CK20</p> Signup and view all the answers

Which urine test should be ordered in suspected carcinoid syndrome?

<p>5-HIAA</p> Signup and view all the answers

What is the gene associated with early onset sarcoid?

<p>CARD15 (NOD2) gene</p> Signup and view all the answers

What are the immunohistochemical markers for shwanomma or Neurolemmoma?

<p>S100+ve, MBP +ve, vimentin +ve</p> Signup and view all the answers

What are the immunohistochemical markers for melanoma?

<p>S100 +ve, HMB45+ve, vimentin +ve</p> Signup and view all the answers

What stain is useful in identifying fungi, parasites, glycogen, and the basement membrane?

<p>PAS stain</p> Signup and view all the answers

What stain is used to identify mastocytosis?

<p>CKIT</p> Signup and view all the answers

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