Podcast
Questions and Answers
Which stain is used in histology that binds to acidic structures and appears blue?
Which stain is used in histology that binds to acidic structures and appears blue?
What is the shortest distance at which two points can be seen as separate called in microscopy?
What is the shortest distance at which two points can be seen as separate called in microscopy?
Which type of electron microscope provides a 3-dimensional view of a structure or cell?
Which type of electron microscope provides a 3-dimensional view of a structure or cell?
What is the name of the red acid dye that binds to basic structures in histologic staining?
What is the name of the red acid dye that binds to basic structures in histologic staining?
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Which heavy metal is typically used to coat a specimen in scanning electron microscopy (SEM)?
Which heavy metal is typically used to coat a specimen in scanning electron microscopy (SEM)?
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What happens when a sample is viewed beyond the resolving power of the microscope?
What happens when a sample is viewed beyond the resolving power of the microscope?
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Name the process in histology where tissue is made firm enough to allow very thin slices to be made.
Name the process in histology where tissue is made firm enough to allow very thin slices to be made.
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Which part of the light microscope magnifies the image from the objective lens?
Which part of the light microscope magnifies the image from the objective lens?
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What is the most commonly used fixative in the preparation of histologic specimens?
What is the most commonly used fixative in the preparation of histologic specimens?
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Which process follows fixation and involves running tissue samples through a series of increasing concentrations of organic solvent?
Which process follows fixation and involves running tissue samples through a series of increasing concentrations of organic solvent?
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What is the name of the stain that colors glycogen and carbohydrate-rich molecules magenta?
What is the name of the stain that colors glycogen and carbohydrate-rich molecules magenta?
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Identify the step in histologic specimen preparation where xylene is generally used.
Identify the step in histologic specimen preparation where xylene is generally used.
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Name the step in histologic specimen preparation that involves placing the specimen on a slide warmer.
Name the step in histologic specimen preparation that involves placing the specimen on a slide warmer.
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Which type of electron microscope produces a negative image on electron-sensitive film?
Which type of electron microscope produces a negative image on electron-sensitive film?
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What material is used to wipe the surface of the glass slide during mounting?
What material is used to wipe the surface of the glass slide during mounting?
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What is the total magnification when using High Power Objective (HPO) at 10x eyepiece lens?
What is the total magnification when using High Power Objective (HPO) at 10x eyepiece lens?
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Study Notes
Histology and Microscopy Basics
- Acidic structures in histology are specifically stained by Hematoxylin, which appears blue.
- The shortest distance at which two points can be seen as separate in microscopy is termed resolution.
- The most commonly used fixative for histologic specimens is Formalin.
- Scanning Electron Microscopy (SEM) provides a three-dimensional view of structures or cells.
- Xylene is typically used during the deparaffinization step in histologic specimen preparation.
- The red acid dye used to stain basic structures in histology is Eosin.
- Fixation involves using protein coagulants or cross-linking agents to preserve cellular structures maximally.
- After fixation, tissues are subjected to a series of increasing concentrations of organic solvent in the dehydration process.
- The stain used to color glycogen and carbohydrate-rich molecules is known as Periodic Acid-Schiff (PAS), which appears magenta.
- The step of placing the specimen on a slide warmer is referred to as mounting.
- Gold is a heavy metal commonly used to coat specimens in Scanning Electron Microscopy (SEM).
- Argyrophilic staining is used to color reticular fibers black in histological preparations.
- Lens paper is used to wipe the surface of the glass slide during mounting.
- The slide warmer facilitates drying and keeps the specimen flat during mounting.
- The process of making tissue firm enough for very thin slices is called embedding.
- The part of the light microscope that magnifies the image from the objective lens is the ocular lens.
- Transmission Electron Microscopy (TEM) produces a negative image on electron-sensitive film.
- The step in preparing histologic specimens following embedding is sectioning, involving trimming and slicing the paraffin-embedded tissue.
- Gross anatomy is the branch dealing with the macroscopic morphology of the human body, visible to the naked eye.
- The nucleus in a cell is typically stained blue by Hematoxylin due to its acidic nature.
- Drying specimens and ensuring they remain flat on the slide is part of the mounting step in specimen preparation.
- High Power Objective (HPO) is used under a 10x eyepiece lens microscope, yielding a total magnification of 100x.
- Oil immersion lenses, often at 100x magnification, are used when studying very minute parts of specimens like individual cells.
- Viewing a sample beyond the resolving power of the microscope results in a blurred image with indistinct features.
- Types of microscopes include light microscopes, electron microscopes, fluorescence microscopes, and confocal microscopes.
- The compound microscope classification refers to the combination of multiple lenses used for magnification.
- Light microscopes form images by passing light through specimens and collecting it via lenses that magnify.
- Objective lenses typically range from 4x (scanning) to 100x (oil immersion) magnification.
- Ocular lenses come in various types, including 10x, and sometimes 15x or 20x magnification.
- Total magnification is calculated by multiplying the eyepiece magnification by the objective lens magnification.
- True: biological specimens usually lack high contrast, necessitating staining for easier identification of parts.
- The specific stains used in histology photomicrographs need to be identified based on the visual characteristics of the specimen.
- Steps in preparing histologic specimens using Hematoxylin and Eosin (H&E) staining generally include fixation, dehydration, clearing, embedding, sectioning, and staining.
- Two main types of electron microscopes are Scanning Electron Microscopes (SEM) and Transmission Electron Microscopes (TEM).
- Important components for histologic specimen preparation include fixatives (e.g., formalin), dehydrating agents (e.g., ethanol), embedding media (e.g., paraffin), and staining solutions (e.g., H&E).
Histology and Microscopy Basics
- Acidic structures in histology are specifically stained by Hematoxylin, which appears blue.
- The shortest distance at which two points can be seen as separate in microscopy is termed resolution.
- The most commonly used fixative for histologic specimens is Formalin.
- Scanning Electron Microscopy (SEM) provides a three-dimensional view of structures or cells.
- Xylene is typically used during the deparaffinization step in histologic specimen preparation.
- The red acid dye used to stain basic structures in histology is Eosin.
- Fixation involves using protein coagulants or cross-linking agents to preserve cellular structures maximally.
- After fixation, tissues are subjected to a series of increasing concentrations of organic solvent in the dehydration process.
- The stain used to color glycogen and carbohydrate-rich molecules is known as Periodic Acid-Schiff (PAS), which appears magenta.
- The step of placing the specimen on a slide warmer is referred to as mounting.
- Gold is a heavy metal commonly used to coat specimens in Scanning Electron Microscopy (SEM).
- Argyrophilic staining is used to color reticular fibers black in histological preparations.
- Lens paper is used to wipe the surface of the glass slide during mounting.
- The slide warmer facilitates drying and keeps the specimen flat during mounting.
- The process of making tissue firm enough for very thin slices is called embedding.
- The part of the light microscope that magnifies the image from the objective lens is the ocular lens.
- Transmission Electron Microscopy (TEM) produces a negative image on electron-sensitive film.
- The step in preparing histologic specimens following embedding is sectioning, involving trimming and slicing the paraffin-embedded tissue.
- Gross anatomy is the branch dealing with the macroscopic morphology of the human body, visible to the naked eye.
- The nucleus in a cell is typically stained blue by Hematoxylin due to its acidic nature.
- Drying specimens and ensuring they remain flat on the slide is part of the mounting step in specimen preparation.
- High Power Objective (HPO) is used under a 10x eyepiece lens microscope, yielding a total magnification of 100x.
- Oil immersion lenses, often at 100x magnification, are used when studying very minute parts of specimens like individual cells.
- Viewing a sample beyond the resolving power of the microscope results in a blurred image with indistinct features.
- Types of microscopes include light microscopes, electron microscopes, fluorescence microscopes, and confocal microscopes.
- The compound microscope classification refers to the combination of multiple lenses used for magnification.
- Light microscopes form images by passing light through specimens and collecting it via lenses that magnify.
- Objective lenses typically range from 4x (scanning) to 100x (oil immersion) magnification.
- Ocular lenses come in various types, including 10x, and sometimes 15x or 20x magnification.
- Total magnification is calculated by multiplying the eyepiece magnification by the objective lens magnification.
- True: biological specimens usually lack high contrast, necessitating staining for easier identification of parts.
- The specific stains used in histology photomicrographs need to be identified based on the visual characteristics of the specimen.
- Steps in preparing histologic specimens using Hematoxylin and Eosin (H&E) staining generally include fixation, dehydration, clearing, embedding, sectioning, and staining.
- Two main types of electron microscopes are Scanning Electron Microscopes (SEM) and Transmission Electron Microscopes (TEM).
- Important components for histologic specimen preparation include fixatives (e.g., formalin), dehydrating agents (e.g., ethanol), embedding media (e.g., paraffin), and staining solutions (e.g., H&E).
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Description
Test your knowledge on essential histology concepts, including stains, fixatives, and microscopy techniques. This quiz covers fundamental questions that every histology student should know to excel in the field. Prepare to challenge your understanding of histological specimen preparation and electron microscopy.
