Spliceosome and RNA Splicing Quiz

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Which proteins are part of the spliceosome complex?

Brr2, Prp8, Prp31

DnaA, DnaB, DnaC

Prp3, Prp6, Prp9 (correct)

Hsp70, Hsp90, Hsp40

What is responsible for removing introns from pre-mRNA?

Ribosomes

Polysomes

Spliceosome (correct)

tRNA synthetase

Which components make up a spliceosome?

snRNPs and mRNA

snRNPs and tRNA

snRNAs and DNA polymerase

snRNPs and snRNAs (correct)

What is the main function of snRNPs in splicing?

Removing introns from pre-mRNA Signup and view all the answers

How does the composition of the spliceosome change during splicing?

New snRNPs are added and removed as splicing progresses Signup and view all the answers

Which process contributes to genetic diversity in eukaryotes?

Intron retention during splicing Signup and view all the answers

What is the function of the carboxyl terminal domain (CTD) of the largest subunit of RNA Pol II in pre-mRNA processing?

Acts as an assembly platform and regulator of pre-mRNA processing machinery Signup and view all the answers

Which process can explain why human cells produce over 100,000 distinct polypeptides despite having around 22,000 genes?

Alternative splicing Signup and view all the answers

What is the major role of alternative splicing in human genes?

Expands the genetic diversity of an organism Signup and view all the answers

Which of the following is NOT a mechanism for producing alternative transcripts?

Alternative 5' capping sites Signup and view all the answers

Which process allows the expression of select transcripts in a tissue-specific manner?

Alternative splicing Signup and view all the answers

What can occur when more than one sequence upstream of a gene can initiate transcription?

Alternative promoters Signup and view all the answers

How do thyroid cells and neuronal cells differ in their processing of the CT/CGRP gene?

Neuronal cells splice out exon 4 to produce CGRP, while thyroid cells use exon 4 to produce calcitonin. Signup and view all the answers

What percentage of human genes are thought to undergo alternative splicing?

~70% Signup and view all the answers

What is the role of RNA Triphosphatase in mRNA processing?

Removing a phosphate from the 5¢ end of the mRNA Signup and view all the answers

What is the function of Guanylyl transferase in mRNA capping?

Joining a guanine monophosphate to the mRNA by a 5¢-to-5¢ triphosphate linkage Signup and view all the answers

Which enzyme adds 20 to 200 adenines to the 3¢ end of pre-mRNA during polyadenylation?

Poly (A) Polymerase (PAP) Signup and view all the answers

What is the purpose of polyadenylation in mRNA processing?

Facilitates transport of mRNA out of the nucleus Signup and view all the answers

What is the main function of the 5¢ cap added to mRNA?

Essential for binding the ribosome on mRNA Signup and view all the answers

What is the function of Torpedo RNase in transcription termination?

Digests residual transcript attached to RNA Polymerase II Signup and view all the answers

What happens after 3¢ cleavage and polyadenylation in transcription termination according to the Torpedo Model?

RNA Polymerase II remains attached to residual transcript Signup and view all the answers

What do introns represent in pre-mRNA processing?

Regions that must be removed to form mature mRNA Signup and view all the answers