Spliceosome and RNA Splicing Quiz

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Questions and Answers

Which proteins are part of the spliceosome complex?

  • Brr2, Prp8, Prp31
  • DnaA, DnaB, DnaC
  • Prp3, Prp6, Prp9 (correct)
  • Hsp70, Hsp90, Hsp40

What is responsible for removing introns from pre-mRNA?

  • Ribosomes
  • Polysomes
  • Spliceosome (correct)
  • tRNA synthetase

Which components make up a spliceosome?

  • snRNPs and mRNA
  • snRNPs and tRNA
  • snRNAs and DNA polymerase
  • snRNPs and snRNAs (correct)

What is the main function of snRNPs in splicing?

<p>Removing introns from pre-mRNA (D)</p> Signup and view all the answers

How does the composition of the spliceosome change during splicing?

<p>New snRNPs are added and removed as splicing progresses (A)</p> Signup and view all the answers

Which process contributes to genetic diversity in eukaryotes?

<p>Intron retention during splicing (C)</p> Signup and view all the answers

What is the function of the carboxyl terminal domain (CTD) of the largest subunit of RNA Pol II in pre-mRNA processing?

<p>Acts as an assembly platform and regulator of pre-mRNA processing machinery (C)</p> Signup and view all the answers

Which process can explain why human cells produce over 100,000 distinct polypeptides despite having around 22,000 genes?

<p>Alternative splicing (B)</p> Signup and view all the answers

What is the major role of alternative splicing in human genes?

<p>Expands the genetic diversity of an organism (A)</p> Signup and view all the answers

Which of the following is NOT a mechanism for producing alternative transcripts?

<p>Alternative 5' capping sites (B)</p> Signup and view all the answers

Which process allows the expression of select transcripts in a tissue-specific manner?

<p>Alternative splicing (D)</p> Signup and view all the answers

What can occur when more than one sequence upstream of a gene can initiate transcription?

<p>Alternative promoters (C)</p> Signup and view all the answers

How do thyroid cells and neuronal cells differ in their processing of the CT/CGRP gene?

<p>Neuronal cells splice out exon 4 to produce CGRP, while thyroid cells use exon 4 to produce calcitonin. (C)</p> Signup and view all the answers

What percentage of human genes are thought to undergo alternative splicing?

<p>~70% (A)</p> Signup and view all the answers

What is the role of RNA Triphosphatase in mRNA processing?

<p>Removing a phosphate from the 5¢ end of the mRNA (C)</p> Signup and view all the answers

What is the function of Guanylyl transferase in mRNA capping?

<p>Joining a guanine monophosphate to the mRNA by a 5¢-to-5¢ triphosphate linkage (A)</p> Signup and view all the answers

Which enzyme adds 20 to 200 adenines to the 3¢ end of pre-mRNA during polyadenylation?

<p>Poly (A) Polymerase (PAP) (A)</p> Signup and view all the answers

What is the purpose of polyadenylation in mRNA processing?

<p>Facilitates transport of mRNA out of the nucleus (C)</p> Signup and view all the answers

What is the main function of the 5¢ cap added to mRNA?

<p>Essential for binding the ribosome on mRNA (A)</p> Signup and view all the answers

What is the function of Torpedo RNase in transcription termination?

<p>Digests residual transcript attached to RNA Polymerase II (C)</p> Signup and view all the answers

What happens after 3¢ cleavage and polyadenylation in transcription termination according to the Torpedo Model?

<p>RNA Polymerase II remains attached to residual transcript (D)</p> Signup and view all the answers

What do introns represent in pre-mRNA processing?

<p>Regions that must be removed to form mature mRNA (A)</p> Signup and view all the answers

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