Spectrophotometer and Spectrophotometry Quiz
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Questions and Answers

What is the basic principle of spectrophotometry?

  • Measuring how much a chemical substance absorbs light by measuring the intensity of light as it passes through a sample solution (correct)
  • Measuring the wavelength of light as it passes through a sample solution
  • Quantifying the concentration of a chemical substance by measuring the color change of the sample solution
  • Determining the volume of a chemical substance in a sample solution

    • What is the unit of measuring wavelength in spectrophotometry?

  • Micrometer (µm)
  • Nanometer (nm) (correct)
  • Millimeter (mm)
  • Centimeter (cm)

    • What type of light does a UV range spectrophotometer use?

  • Light over the X-ray range, with very short wavelengths
  • Light over the ultraviolet range, with wavelength ranges between 185 - 400 nm (correct)
  • Light over the visible range, with wavelength ranges between 400 - 700 nm
  • Light over the infrared range, with wavelength ranges above 700 nm

    • What does a monochromator do in a spectrophotometer?

    <p>Absorbs most of the light and allows only light of the complementary color with a sufficiently narrow wavelength</p> Signup and view all the answers

    What is the function of the light source in a spectrophotometer?

    <p>Emits light in either the visible or ultraviolet range depending on the type of spectrophotometer</p> Signup and view all the answers

    What is the meaning of the term 'microscope'?

    <p>An instrument for inspecting small objects</p> Signup and view all the answers

    What happens if there is increased magnification without improved resolution?

    <p>Results in a large blurred image</p> Signup and view all the answers

    What determines the total magnification in a microscope?

    <p>Power of ocular and power of objective</p> Signup and view all the answers

    What is the resolving power of the unaided human eye?

    <p>0.1 mm or 100 μm</p> Signup and view all the answers

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