Spectrophotometer and Spectrophotometry

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What is the basic principle of spectrophotometry?

Determining the weight of a chemical substance by analyzing light absorption

Measuring the speed of light as it passes through a sample solution

Quantifying the volume of a chemical substance based on light intensity

Measuring how much a chemical substance absorbs light by measuring the intensity of light as the beam of light passes through a sample solution (correct)

What is the unit of measuring wavelength in spectrophotometry?

Micrometer (µm)

Millimeter (mm)

Centimeter (cm)

Nanometer (nm) (correct)

What type of light does a UV range spectrophotometer use?

Light over the infrared range, with wavelength ranges between 700 - 1000 nm

Light over the ultraviolet range, with wavelength ranges between 185 - 400 nm (correct)

Light over the X-ray range, with wavelength ranges above 1 nm

Light over the visible range, with wavelength ranges between 400 - 700 nm

What is the function of the tungsten lamp in a spectrophotometer?

<p>To emit light in the visible range only as the light source</p> Signup and view all the answers

What does a monochromator do in a spectrophotometer?

<p>Absorbs most of the light and allows only light of the complementary color with a sufficiently narrow wavelength</p> Signup and view all the answers