Uzma Chapter 4_mcq_create with chatgpt4
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Questions and Answers

What is the primary role of DNA polymerase in PCR (Polymerase Chain Reaction)?

  • To cleave DNA fragments
  • To replicate a specific segment of DNA (correct)
  • To join DNA fragments
  • To transcribe DNA into RNA

    • Which technique allows the amplification of a specific segment of DNA?

  • Gel electrophoresis
  • Southern blotting
  • PCR (Polymerase Chain Reaction) (correct)
  • DNA sequencing

    • What is the significance of recombinant DNA technology?

  • It allows the study of mutant phenotypes.
  • It enables the cloning of specific DNA fragments in bacteria. (correct)
  • It is used for amplifying DNA segments.
  • It is the primary method for DNA sequencing.

    • Which process involves the propagation of individual DNA fragments in bacteria for isolation?

    <p>Molecular cloning</p> Signup and view all the answers

    What is a key feature of the DNA Provirus Hypothesis?

    <p>It suggests that viral DNA integrates into the host genome.</p> Signup and view all the answers

    Which of the following best describes the structure of DNA?

    <p>Double-stranded helix</p> Signup and view all the answers

    In the context of gene expression, what is the primary function of RNA interference (RNAi)?

    <p>Silencing gene expression</p> Signup and view all the answers

    What is the main purpose of using restriction endonucleases in recombinant DNA technology?

    <p>To cut DNA at specific sequences</p> Signup and view all the answers

    Which experimental evidence supports the semiconservative replication of DNA?

    <p>The Meselson-Stahl experiment</p> Signup and view all the answers

    What role does a plasmid vector play in molecular cloning?

    <p>It carries foreign DNA into a host cell.</p> Signup and view all the answers

    Which experiment established DNA as the genetic material?

    <p>The Hershey-Chase experiment</p> Signup and view all the answers

    What is the primary method for detecting specific nucleic acids within a sample?

    <p>Southern blotting</p> Signup and view all the answers

    In gene expression, which process involves the transfer of genetic information from DNA to RNA?

    <p>Transcription</p> Signup and view all the answers

    What is the role of dideoxynucleotides in DNA sequencing?

    <p>They terminate DNA chain elongation.</p> Signup and view all the answers

    Which method is used for amplifying a single DNA molecule to a detectable quantity?

    <p>Polymerase Chain Reaction (PCR)</p> Signup and view all the answers

    What is the fundamental property of all living things that is emphasized in the study of heredity?

    <p>The ability to reproduce</p> Signup and view all the answers

    Which is a key component in the expression of genetic information in eukaryotes?

    <p>RNA interference</p> Signup and view all the answers

    What is the significance of the DNA Provirus Hypothesis in molecular biology?

    <p>It suggests viral DNA can integrate into the host genome.</p> Signup and view all the answers

    What does the Meselson-Stahl experiment demonstrate about DNA replication?

    <p>It is a semi-conservative process.</p> Signup and view all the answers

    Which component is essential for the replication of DNA during PCR?

    <p>Taq polymerase</p> Signup and view all the answers

    Which technique involves the separation of DNA fragments based on size?

    <p>Gel electrophoresis</p> Signup and view all the answers

    In genetic engineering, what is the function of a plasmid vector?

    <p>To introduce foreign DNA into host cells</p> Signup and view all the answers

    Which experiment demonstrated that DNA, not protein, is the genetic material?

    <p>The Hershey-Chase experiment</p> Signup and view all the answers

    What is the main purpose of RNA interference (RNAi) in eukaryotic cells?

    <p>Gene silencing</p> Signup and view all the answers

    Which method is used to determine the sequence of nucleotides in a DNA molecule?

    <p>DNA sequencing</p> Signup and view all the answers

    Which enzyme is used to cut DNA at specific sequences in recombinant DNA technology?

    <p>Restriction endonuclease</p> Signup and view all the answers

    What is the primary role of gel electrophoresis in molecular biology?

    <p>Separating DNA fragments</p> Signup and view all the answers

    In the context of gene function in eukaryotes, what is a major advantage of recombinant DNA technology?

    <p>It allows for the study of genes within intact organisms.</p> Signup and view all the answers

    Which process in PCR results in an exponential increase in the number of DNA molecules?

    <p>All of the above</p> Signup and view all the answers

    What is the significance of the Avery-MacLeod-McCarty experiment in molecular biology?

    <p>It demonstrated that DNA is the genetic material.</p> Signup and view all the answers

    In eukaryotes, which process involves the removal of introns and joining of exons in mRNA?

    <p>RNA splicing</p> Signup and view all the answers

    What is the purpose of DNA ligase in recombinant DNA technology?

    <p>To join together DNA fragments</p> Signup and view all the answers

    Which method is typically used for the detection of proteins?

    <p>Western blotting</p> Signup and view all the answers

    What is the significance of the polymerase chain reaction (PCR) in molecular biology?

    <p>It is used for the amplification of specific DNA segments.</p> Signup and view all the answers

    Which statement best describes the role of RNA interference (RNAi) in gene expression?

    <p>It silences specific genes by degrading mRNA.</p> Signup and view all the answers

    In molecular cloning, what is the primary function of a restriction endonuclease?

    <p>To cut DNA at specific recognition sites</p> Signup and view all the answers

    What is the main function of Taq polymerase in PCR?

    <p>To synthesize new DNA strands</p> Signup and view all the answers

    Which of the following best describes the process of gel electrophoresis?

    <p>Separation of molecules based on size and charge</p> Signup and view all the answers

    What is the primary goal of Southern blotting in molecular biology?

    <p>To detect specific sequences of DNA</p> Signup and view all the answers

    Which experiment was crucial in demonstrating the semiconservative model of DNA replication?

    <p>The Meselson-Stahl experiment</p> Signup and view all the answers

    In the context of molecular cloning, what is the purpose of a vector?

    <p>To carry and replicate foreign DNA within a host cell.</p> Signup and view all the answers

    Which technique is used to identify specific proteins within a complex mixture?

    <p>Western blotting</p> Signup and view all the answers

    What role do dideoxynucleotides play in DNA sequencing?

    <p>They terminate DNA chain elongation.</p> Signup and view all the answers

    In the process of RNA splicing, what is removed from the pre-mRNA molecule?

    <p>Introns</p> Signup and view all the answers

    What is the function of restriction endonucleases in recombinant DNA technology?

    <p>To cut DNA at specific sequences</p> Signup and view all the answers

    Which statement best describes the role of Taq polymerase in PCR?

    <p>It synthesizes new strands of DNA.</p> Signup and view all the answers

    What is the main application of gel electrophoresis in molecular biology?

    <p>To separate molecules based on size and charge</p> Signup and view all the answers

    In molecular biology, what is the primary purpose of Southern blotting?

    <p>To detect specific sequences of DNA</p> Signup and view all the answers

    Which process in the polymerase chain reaction (PCR) involves the binding of primers to the target DNA sequence?

    <p>Annealing</p> Signup and view all the answers

    What is the significance of the Meselson-Stahl experiment in understanding DNA replication?

    <p>It demonstrated that DNA replicates in a semi-conservative manner.</p> Signup and view all the answers

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