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Questions and Answers
What is the primary role of DNA polymerase in PCR (Polymerase Chain Reaction)?
What is the primary role of DNA polymerase in PCR (Polymerase Chain Reaction)?
- To cleave DNA fragments
- To replicate a specific segment of DNA (correct)
- To join DNA fragments
- To transcribe DNA into RNA
Which technique allows the amplification of a specific segment of DNA?
Which technique allows the amplification of a specific segment of DNA?
- Gel electrophoresis
- Southern blotting
- PCR (Polymerase Chain Reaction) (correct)
- DNA sequencing
What is the significance of recombinant DNA technology?
What is the significance of recombinant DNA technology?
- It allows the study of mutant phenotypes.
- It enables the cloning of specific DNA fragments in bacteria. (correct)
- It is used for amplifying DNA segments.
- It is the primary method for DNA sequencing.
Which process involves the propagation of individual DNA fragments in bacteria for isolation?
Which process involves the propagation of individual DNA fragments in bacteria for isolation?
What is a key feature of the DNA Provirus Hypothesis?
What is a key feature of the DNA Provirus Hypothesis?
Which of the following best describes the structure of DNA?
Which of the following best describes the structure of DNA?
In the context of gene expression, what is the primary function of RNA interference (RNAi)?
In the context of gene expression, what is the primary function of RNA interference (RNAi)?
What is the main purpose of using restriction endonucleases in recombinant DNA technology?
What is the main purpose of using restriction endonucleases in recombinant DNA technology?
Which experimental evidence supports the semiconservative replication of DNA?
Which experimental evidence supports the semiconservative replication of DNA?
What role does a plasmid vector play in molecular cloning?
What role does a plasmid vector play in molecular cloning?
Which experiment established DNA as the genetic material?
Which experiment established DNA as the genetic material?
What is the primary method for detecting specific nucleic acids within a sample?
What is the primary method for detecting specific nucleic acids within a sample?
In gene expression, which process involves the transfer of genetic information from DNA to RNA?
In gene expression, which process involves the transfer of genetic information from DNA to RNA?
What is the role of dideoxynucleotides in DNA sequencing?
What is the role of dideoxynucleotides in DNA sequencing?
Which method is used for amplifying a single DNA molecule to a detectable quantity?
Which method is used for amplifying a single DNA molecule to a detectable quantity?
What is the fundamental property of all living things that is emphasized in the study of heredity?
What is the fundamental property of all living things that is emphasized in the study of heredity?
Which is a key component in the expression of genetic information in eukaryotes?
Which is a key component in the expression of genetic information in eukaryotes?
What is the significance of the DNA Provirus Hypothesis in molecular biology?
What is the significance of the DNA Provirus Hypothesis in molecular biology?
What does the Meselson-Stahl experiment demonstrate about DNA replication?
What does the Meselson-Stahl experiment demonstrate about DNA replication?
Which component is essential for the replication of DNA during PCR?
Which component is essential for the replication of DNA during PCR?
Which technique involves the separation of DNA fragments based on size?
Which technique involves the separation of DNA fragments based on size?
In genetic engineering, what is the function of a plasmid vector?
In genetic engineering, what is the function of a plasmid vector?
Which experiment demonstrated that DNA, not protein, is the genetic material?
Which experiment demonstrated that DNA, not protein, is the genetic material?
What is the main purpose of RNA interference (RNAi) in eukaryotic cells?
What is the main purpose of RNA interference (RNAi) in eukaryotic cells?
Which method is used to determine the sequence of nucleotides in a DNA molecule?
Which method is used to determine the sequence of nucleotides in a DNA molecule?
Which enzyme is used to cut DNA at specific sequences in recombinant DNA technology?
Which enzyme is used to cut DNA at specific sequences in recombinant DNA technology?
What is the primary role of gel electrophoresis in molecular biology?
What is the primary role of gel electrophoresis in molecular biology?
In the context of gene function in eukaryotes, what is a major advantage of recombinant DNA technology?
In the context of gene function in eukaryotes, what is a major advantage of recombinant DNA technology?
Which process in PCR results in an exponential increase in the number of DNA molecules?
Which process in PCR results in an exponential increase in the number of DNA molecules?
What is the significance of the Avery-MacLeod-McCarty experiment in molecular biology?
What is the significance of the Avery-MacLeod-McCarty experiment in molecular biology?
In eukaryotes, which process involves the removal of introns and joining of exons in mRNA?
In eukaryotes, which process involves the removal of introns and joining of exons in mRNA?
What is the purpose of DNA ligase in recombinant DNA technology?
What is the purpose of DNA ligase in recombinant DNA technology?
Which method is typically used for the detection of proteins?
Which method is typically used for the detection of proteins?
What is the significance of the polymerase chain reaction (PCR) in molecular biology?
What is the significance of the polymerase chain reaction (PCR) in molecular biology?
Which statement best describes the role of RNA interference (RNAi) in gene expression?
Which statement best describes the role of RNA interference (RNAi) in gene expression?
In molecular cloning, what is the primary function of a restriction endonuclease?
In molecular cloning, what is the primary function of a restriction endonuclease?
What is the main function of Taq polymerase in PCR?
What is the main function of Taq polymerase in PCR?
Which of the following best describes the process of gel electrophoresis?
Which of the following best describes the process of gel electrophoresis?
What is the primary goal of Southern blotting in molecular biology?
What is the primary goal of Southern blotting in molecular biology?
Which experiment was crucial in demonstrating the semiconservative model of DNA replication?
Which experiment was crucial in demonstrating the semiconservative model of DNA replication?
In the context of molecular cloning, what is the purpose of a vector?
In the context of molecular cloning, what is the purpose of a vector?
Which technique is used to identify specific proteins within a complex mixture?
Which technique is used to identify specific proteins within a complex mixture?
What role do dideoxynucleotides play in DNA sequencing?
What role do dideoxynucleotides play in DNA sequencing?
In the process of RNA splicing, what is removed from the pre-mRNA molecule?
In the process of RNA splicing, what is removed from the pre-mRNA molecule?
What is the function of restriction endonucleases in recombinant DNA technology?
What is the function of restriction endonucleases in recombinant DNA technology?
Which statement best describes the role of Taq polymerase in PCR?
Which statement best describes the role of Taq polymerase in PCR?
What is the main application of gel electrophoresis in molecular biology?
What is the main application of gel electrophoresis in molecular biology?
In molecular biology, what is the primary purpose of Southern blotting?
In molecular biology, what is the primary purpose of Southern blotting?
Which process in the polymerase chain reaction (PCR) involves the binding of primers to the target DNA sequence?
Which process in the polymerase chain reaction (PCR) involves the binding of primers to the target DNA sequence?
What is the significance of the Meselson-Stahl experiment in understanding DNA replication?
What is the significance of the Meselson-Stahl experiment in understanding DNA replication?
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