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Questions and Answers
What is the main purpose of the polymerase chain reaction (PCR)?
What is the main purpose of the polymerase chain reaction (PCR)?
- To separate DNA strands
- To amplify a specific segment of DNA (correct)
- To synthesize new DNA strands from scratch
- To analyze the entire genome of an organism
What is the role of the enzyme DNA polymerase in PCR?
What is the role of the enzyme DNA polymerase in PCR?
- To identify mutations in the DNA sequence
- To break down DNA into its individual nucleotides
- To add nucleotides to the growing DNA strand (correct)
- To prevent unwanted amplification of DNA
Which step in PCR involves heating the reaction to separate the DNA strands?
Which step in PCR involves heating the reaction to separate the DNA strands?
- Denaturation (correct)
- Extension
- Polymerization
- Annealing
Why is a thermal cycler used in PCR?
Why is a thermal cycler used in PCR?
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