Polymerase Chain Reaction (PCR) Overview
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Questions and Answers

What is the main purpose of the polymerase chain reaction (PCR)?

  • To separate DNA strands
  • To amplify a specific segment of DNA (correct)
  • To synthesize new DNA strands from scratch
  • To analyze the entire genome of an organism
  • What is the role of the enzyme DNA polymerase in PCR?

  • To identify mutations in the DNA sequence
  • To break down DNA into its individual nucleotides
  • To add nucleotides to the growing DNA strand (correct)
  • To prevent unwanted amplification of DNA
  • Which step in PCR involves heating the reaction to separate the DNA strands?

  • Denaturation (correct)
  • Extension
  • Polymerization
  • Annealing
  • Why is a thermal cycler used in PCR?

    <p>To control the temperature changes in the PCR process</p> Signup and view all the answers

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