Polymerase Chain Reaction (PCR) Overview

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What is the main purpose of the polymerase chain reaction (PCR)?

To amplify a specific segment of DNA

What is the role of the enzyme DNA polymerase in PCR?

To add nucleotides to the growing DNA strand

Which step in PCR involves heating the reaction to separate the DNA strands?

Denaturation

Why is a thermal cycler used in PCR?

To control the temperature changes in the PCR process

Test your knowledge about the main purpose of the polymerase chain reaction, the role of DNA polymerase, the step involving heating to separate DNA strands, and the use of a thermal cycler in PCR.

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