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Questions and Answers
What is the purpose of using primers in the PCR process?
What is the purpose of using primers in the PCR process?
- To amplify the DNA sequence
- To cool the reaction mixture
- To serve as a starting point for DNA replication (correct)
- To denature the DNA template
Which enzyme is commonly used in PCR for DNA amplification?
Which enzyme is commonly used in PCR for DNA amplification?
- Taq polymerase (correct)
- RNA polymerase
- Helicase
- DNA ligase
At what temperature does denaturation typically occur in the PCR process?
At what temperature does denaturation typically occur in the PCR process?
- 37°C
- -20°C
- 50-65°C
- 94°C (correct)
What is the function of the thermal cycler (PCR machine) in PCR?
What is the function of the thermal cycler (PCR machine) in PCR?
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What does a pair of synthetic oligonucleotide primers do in a PCR reaction mixture?
What does a pair of synthetic oligonucleotide primers do in a PCR reaction mixture?
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Which enzyme assembles a new DNA strand from nucleotides in PCR?
Which enzyme assembles a new DNA strand from nucleotides in PCR?
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What is the main function of Taq polymerase in PCR?
What is the main function of Taq polymerase in PCR?
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