Polymerase Chain Reaction (PCR)
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Questions and Answers

What is the purpose of using primers in the PCR process?

  • To amplify the DNA sequence
  • To cool the reaction mixture
  • To serve as a starting point for DNA replication (correct)
  • To denature the DNA template
  • Which enzyme is commonly used in PCR for DNA amplification?

  • Taq polymerase (correct)
  • RNA polymerase
  • Helicase
  • DNA ligase
  • At what temperature does denaturation typically occur in the PCR process?

  • 37°C
  • -20°C
  • 50-65°C
  • 94°C (correct)
  • What is the function of the thermal cycler (PCR machine) in PCR?

    <p>To control the temperature for different steps of PCR</p> Signup and view all the answers

    What does a pair of synthetic oligonucleotide primers do in a PCR reaction mixture?

    <p>Hybridizes with the template DNA</p> Signup and view all the answers

    Which enzyme assembles a new DNA strand from nucleotides in PCR?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the main function of Taq polymerase in PCR?

    <p>Synthesizes new DNA strands</p> Signup and view all the answers

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