7 Questions
What is the purpose of using primers in the PCR process?
To serve as a starting point for DNA replication
Which enzyme is commonly used in PCR for DNA amplification?
Taq polymerase
At what temperature does denaturation typically occur in the PCR process?
94°C
What is the function of the thermal cycler (PCR machine) in PCR?
To control the temperature for different steps of PCR
What does a pair of synthetic oligonucleotide primers do in a PCR reaction mixture?
Hybridizes with the template DNA
Which enzyme assembles a new DNA strand from nucleotides in PCR?
DNA polymerase
What is the main function of Taq polymerase in PCR?
Synthesizes new DNA strands
Test your knowledge of the in vitro exponential amplification of specific DNA sequences using PCR. Learn about the use of DNA polymerase, thermal cyclers, and the components of a PCR reaction mixture.
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