12 Questions
What is a disadvantage of Northern blotting compared to Digital RNA counting?
Requires radioactive labelling
Why is formaldehyde mentioned in relation to RNA in the text?
To explain its unfolding effect in Northern blotting
What is a key advantage of Digital RNA counting over Northern blotting?
Provides a high throughput of up to 800 targets
Why might Digital RNA counting be considered expensive for small experiments?
Needs a big facility
In Digital RNA counting, what is the function of the capture probe?
Tether the molecule to a glass plate
What is the main limitation of Northern blotting in terms of throughput?
Can only measure a few RNA species at a time
What is the primary purpose of qRT-PCR?
To quantify and detect RNA molecules
What is the main limitation of qRT-PCR mentioned in the text?
It assumes equal cDNA synthesis efficiency across genes
What technique can be used to overcome the limitation of qRT-PCR regarding RNA molecule size?
Northern blotting
What is the first step in the Northern blotting technique?
RNA extraction and denaturation
What is the purpose of pre-hybridization buffer in Northern blotting?
To reduce non-specific binding
What is the final step in the Northern blotting technique described in the text?
Incubation in pre-hybridization buffer
Explore the principles of RNA quantification and detection using quantitative Reverse-transcription PCR (qRT-PCR) and quantitative PCR. Understand how real-time fluorescence is used to determine quantification, along with the limitations of qRT-PCR such as variability in cDNA synthesis efficiency.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
