Podcast
Questions and Answers
What is a disadvantage of Northern blotting compared to Digital RNA counting?
What is a disadvantage of Northern blotting compared to Digital RNA counting?
- Provides information on RNA length
- Requires radioactive labelling (correct)
- Works by using two probes
- Can measure up to 800 targets
Why is formaldehyde mentioned in relation to RNA in the text?
Why is formaldehyde mentioned in relation to RNA in the text?
- To highlight its role as a primer in Digital RNA counting
- To describe its function in cDNA synthesis
- To explain its unfolding effect in Northern blotting (correct)
- To emphasize its use in PCR amplification
What is a key advantage of Digital RNA counting over Northern blotting?
What is a key advantage of Digital RNA counting over Northern blotting?
- Can measure RNA length
- Involves incubation at 42°C
- Requires radioactive labelling
- Provides a high throughput of up to 800 targets (correct)
Why might Digital RNA counting be considered expensive for small experiments?
Why might Digital RNA counting be considered expensive for small experiments?
In Digital RNA counting, what is the function of the capture probe?
In Digital RNA counting, what is the function of the capture probe?
What is the main limitation of Northern blotting in terms of throughput?
What is the main limitation of Northern blotting in terms of throughput?
What is the primary purpose of qRT-PCR?
What is the primary purpose of qRT-PCR?
What is the main limitation of qRT-PCR mentioned in the text?
What is the main limitation of qRT-PCR mentioned in the text?
What technique can be used to overcome the limitation of qRT-PCR regarding RNA molecule size?
What technique can be used to overcome the limitation of qRT-PCR regarding RNA molecule size?
What is the first step in the Northern blotting technique?
What is the first step in the Northern blotting technique?
What is the purpose of pre-hybridization buffer in Northern blotting?
What is the purpose of pre-hybridization buffer in Northern blotting?
What is the final step in the Northern blotting technique described in the text?
What is the final step in the Northern blotting technique described in the text?
