Dengue Diagnostic Tests: NS1 ELISA, Lateral Flow, and qRT-PCR

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Questions and Answers

What is the primary use of dengue NS1 antigen rapid diagnostic test?

To monitor the treatment of dengue patients

To study dengue epidemiology in rural areas

To prevent dengue virus transmission

To detect dengue virus in patients with acute febrile illness (correct)

What is the advantage of using lateral flow rapid tests for dengue diagnosis?

They can be used in difficult-to-reach areas (correct)

They are less expensive than ELISA tests

They require sophisticated laboratory equipment

They are more accurate than ELISA tests

What is the relationship between viraemia and antibody responses in dengue diagnosis?

Viraemia is dependent on antibody responses

Viraemia is inversely related to antibody responses

Viraemia is independent of antibody responses

Viraemia is directly related to antibody responses (correct)

What is the primary goal of evaluating dengue rapid diagnostic tests?

To compare different diagnostic tests Signup and view all the answers

Which of the following is a limitation of using qRT-PCR for dengue diagnosis?

It requires sophisticated laboratory equipment Signup and view all the answers

What is the primary application of nonstructural 1 enzyme-linked immunosorbent assay (NS1 ELISA) in dengue diagnosis?

To detect dengue virus in patients with acute febrile illness Signup and view all the answers

What is the significance of calculating the average cost per case of dengue fever?

To understand the economic burden of dengue fever Signup and view all the answers

What is the primary advantage of using rapid diagnostic tests in rural areas?

They can be used in areas with limited laboratory infrastructure Signup and view all the answers

What is the term used to describe the severe cases of dengue infection?

Dengue hemorrhagic fever (DHF) Signup and view all the answers

In which climates is dengue infection hyperendemic?

Tropical and subtropical climates Signup and view all the answers

What is the case fatality rate of dengue infection in the Philippines?

0.3% Signup and view all the answers

How many cases of dengue were reported in 2019 in the Philippines?

270,000 cases Signup and view all the answers

What is the recommended repellent for the prevention of dengue?

DEET repellents Signup and view all the answers

How many cases of dengue were reported by mid-October 2022 in the Philippines?

181,971 cases Signup and view all the answers

What is the expected trend of dengue infection in the Philippines and worldwide?

Rapidly spreading Signup and view all the answers

What is the primary purpose of the NS1 Ag-based tests for dengue virus?

To detect dengue virus in the Asian population Signup and view all the answers

According to the Ministry of Health Malaysia, what is the recommended approach for managing dengue in children?

Following the Clinical Practice Guidelines for Management of Dengue in Children Signup and view all the answers

What is the primary advantage of commercial dengue rapid diagnostic tests?

Ease of use and rapid results for point-of-care application Signup and view all the answers

What is a common limitation of rapid immunochromatographic tests for dengue diagnosis?

Low sensitivity and specificity in detecting dengue virus Signup and view all the answers

What is the primary focus of the study by Mata et al. (2017)?

Comparing the accuracy of different rapid diagnostic tests for dengue diagnosis Signup and view all the answers

What is the primary objective of the clinical practice guidelines for management of dengue in children?

To provide evidence-based recommendations for managing dengue in children Signup and view all the answers

What is the primary advantage of using commercial dengue rapid diagnostic tests at the point-of-care setting?

Rapid results and ease of use for healthcare professionals Signup and view all the answers

What is the primary focus of the study by Buonora et al. (2016)?

Investigating the accuracy of clinical criteria and immunochromatographic strip test for dengue diagnosis Signup and view all the answers

Study Notes

Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue

Studies have evaluated the diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue.
These tests have been compared to other diagnostic methods, including qRT-PCR, for their sensitivity and specificity in detecting dengue infections.

Evaluation of Commercial Dengue NS1 Antigen Rapid Diagnostic Tests

Various studies have evaluated the performance of commercial dengue NS1 antigen rapid diagnostic tests in different settings.
These tests have shown varying levels of sensitivity and specificity, with some studies reporting high accuracy in detecting dengue infections.

Cost of Dengue Illness to Households

A study in rural Cambodia found that the cost of dengue illness to households was significant, highlighting the economic burden of the disease.

Guideline for Dengue Management

The Ministry of Health Malaysia has published clinical practice guidelines for the management of dengue in children.

Accuracy and Reliability of Rapid Diagnostic Tests

Studies have evaluated the accuracy and reliability of rapid diagnostic tests for dengue, including NS1 antigen-based tests.
These tests have shown varying levels of accuracy and reliability, with some studies highlighting the need for further evaluation.

Prevention of Dengue Infection

Recommendations have been made against the use of certain repellents, such as plant-based non-DEET extracts, IR3535, and citronella, for the prevention of dengue infection.
DEET repellents are recommended for the prevention of dengue infection.

Epidemiology of Dengue in the Philippines

Dengue is a major public health concern in the Philippines, with high incidence rates reported each year.
The disease has a case fatality rate of 0.3% in the Philippines.
The Philippines has reported a high number of dengue cases, with a 91% increase in cases reported in 2022 compared to the previous year.

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Description

This quiz covers the diagnostic accuracy and comparison of different tests for dengue, including NS1 ELISA, lateral flow rapid tests, and qRT-PCR. It also explores their relationship to viraemia and antibody responses.