Restriction Enzymes Quiz
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Questions and Answers

What is the main function of restriction enzymes in bacteria and archaea?

  • Defend against invading viruses by cutting foreign DNA (correct)
  • Regulate gene expression
  • Repair damaged DNA strands
  • Facilitate DNA replication
  • How do restriction enzymes cut DNA?

  • Make two incisions, once through each sugar-phosphate backbone of the DNA double helix (correct)
  • Create multiple incisions at random sites on the DNA strand
  • Break the hydrogen bonds between the nitrogenous bases
  • Remove the phosphate groups from the DNA backbone
  • What is the role of modification enzymes in prokaryotes?

  • Assist in DNA replication
  • Cleave foreign DNA
  • Protect host DNA by modifying it (correct)
  • Facilitate gene transcription
  • What is the broader group of enzymes to which restriction enzymes belong?

    <p>Endonucleases</p> Signup and view all the answers

    How are restriction enzymes commonly classified?

    <p>Into five types based on their structure and cutting mechanism</p> Signup and view all the answers

    Study Notes

    Restriction Enzymes in Prokaryotes

    • The main function of restriction enzymes in bacteria and archaea is to protect the cell from foreign DNA by cutting it into smaller pieces.

    Mechanism of Action

    • Restriction enzymes cut DNA at specific recognition sites, typically 4-8 base pairs in length, into smaller fragments.
    • These recognition sites are usually palindromic, meaning they read the same forwards and backwards.

    Modification Enzymes

    • The role of modification enzymes in prokaryotes is to modify the host's own DNA, making it resistant to the restriction enzymes.

    Classification and Broader Group

    • Restriction enzymes belong to the broader group of endonucleases, which are enzymes that cut within a DNA molecule.
    • Restriction enzymes are commonly classified into three types: Type I, Type II, and Type III, based on their structure, mechanism, and cofactor requirements.

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    Description

    Test your knowledge of restriction enzymes and their classification in this informative quiz. Challenge yourself with questions about recognition sites, types of restriction enzymes, and their structure. See how much you know about these essential tools in molecular biology.

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