5 Questions
What is the main function of a restriction enzyme?
To break phosphodiester bonds in DNA molecules
Which type of enzyme digests nucleotides in the interior of a DNA molecule?
Endonucleases
What do restriction enzymes recognize in DNA before cutting it?
Palindromic sequences
What is the primary role of exonucleases in DNA digestion?
To digest nucleotides from the ends of the DNA molecules
Where were restriction enzymes originally discovered?
In bacteria
Study Notes
Restriction Enzymes
- The main function of a restriction enzyme is to cut DNA at specific recognition sites.
- Restriction enzymes digest nucleotides in the interior of a DNA molecule, not at the ends.
- These enzymes recognize specific DNA sequences, known as recognition sites, before cutting the DNA.
- Recognition sites typically consist of 4-8 nucleotides and are often palindromic.
Exonucleases
- Exonucleases have a primary role in DNA digestion, specifically degrading nucleotides from the ends of DNA molecules.
Discovery of Restriction Enzymes
- Restriction enzymes were originally discovered in bacteria.
Test your knowledge on restriction enzymes with this quiz. Covering topics such as types of restriction enzymes, nomenclature, and applications, this quiz will help reinforce your understanding of these important DNA-cutting tools.
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