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Questions and Answers
What is the main function of a restriction enzyme?
What is the main function of a restriction enzyme?
Which type of enzyme digests nucleotides in the interior of a DNA molecule?
Which type of enzyme digests nucleotides in the interior of a DNA molecule?
What do restriction enzymes recognize in DNA before cutting it?
What do restriction enzymes recognize in DNA before cutting it?
What is the primary role of exonucleases in DNA digestion?
What is the primary role of exonucleases in DNA digestion?
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Where were restriction enzymes originally discovered?
Where were restriction enzymes originally discovered?
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Study Notes
Restriction Enzymes
- The main function of a restriction enzyme is to cut DNA at specific recognition sites.
- Restriction enzymes digest nucleotides in the interior of a DNA molecule, not at the ends.
- These enzymes recognize specific DNA sequences, known as recognition sites, before cutting the DNA.
- Recognition sites typically consist of 4-8 nucleotides and are often palindromic.
Exonucleases
- Exonucleases have a primary role in DNA digestion, specifically degrading nucleotides from the ends of DNA molecules.
Discovery of Restriction Enzymes
- Restriction enzymes were originally discovered in bacteria.
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Description
Test your knowledge on restriction enzymes with this quiz. Covering topics such as types of restriction enzymes, nomenclature, and applications, this quiz will help reinforce your understanding of these important DNA-cutting tools.
