16 Questions
What is the main function of restriction enzymes in gene cloning experiments?
Cutting DNA into smaller strands for analysis
Which of the following is NOT an application of restriction enzymes?
Studying protein synthesis mechanisms
What is the significance of recognition sites like Eco Rl in restriction enzyme technology?
They are specific sites where restriction enzymes cut DNA
In the context of restriction enzymes, what does the term 'RFLP' stand for?
Restriction Fragment Length Polymorphism
How do restriction enzymes contribute to the large-scale production of human insulin using E.coli?
They cut the human insulin gene and insert it into E.coli plasmids
What is the primary function of Type I restriction endonucleases?
Cleave the DNA about 1000 bp away from a specific end of the recognition site
What is the distinguishing feature of blunt end fragments produced by restriction enzymes?
They can be joined to any other DNA fragment with blunt ends
Which co-factor is commonly required for Type I restriction endonucleases to function?
Magnesium (Mg2+)
What is the recognition sequence length for Type II restriction endonucleases?
15 bp
How do restriction enzymes create new molecules from DNA fragments?
By combining DNA fragments with complimentary sticky ends
In what way do Type III restriction endonucleases differ from Type II enzymes?
Cleavage site position relative to the target sequence
What is the main function of restriction endonucleases?
Produce internal cuts in DNA molecules
Who were the scientists awarded the 1978 Nobel Prize for Physiology or Medicine for their work on restriction enzymes discovery and characterization?
Werner Arber, Daniel Nathans, and Hamilton O.Smith
What type of nucleotide sequence do restriction enzymes recognize before making cuts in DNA?
Recognition or restriction sites
Which of the following statements is true about the subunits of restriction enzymes?
They are composed of amino acids
What do bacteria use restriction enzymes for?
To resist viral attack
Test your knowledge on the applications of restriction enzymes in gene cloning, protein expression, and biotechnology. Questions cover topics such as Eco B and EcoR I restriction sites, ATP, S-Adenosyl methionine, and more.
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