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Questions and Answers
What is the main function of restriction enzymes in gene cloning experiments?
What is the main function of restriction enzymes in gene cloning experiments?
- Determining nucleotide sequences
- Increasing the rate of DNA replication
- Integrating genes into a host organism
- Cutting DNA into smaller strands for analysis (correct)
Which of the following is NOT an application of restriction enzymes?
Which of the following is NOT an application of restriction enzymes?
- Producing insulin for diabetics using E.coli
- Creating recombinant DNA
- Cloning DNA molecules
- Studying protein synthesis mechanisms (correct)
What is the significance of recognition sites like Eco Rl in restriction enzyme technology?
What is the significance of recognition sites like Eco Rl in restriction enzyme technology?
- They regulate gene expression
- They are specific sites where restriction enzymes cut DNA (correct)
- They indicate the start of a gene sequence
- They help in DNA replication
In the context of restriction enzymes, what does the term 'RFLP' stand for?
In the context of restriction enzymes, what does the term 'RFLP' stand for?
How do restriction enzymes contribute to the large-scale production of human insulin using E.coli?
How do restriction enzymes contribute to the large-scale production of human insulin using E.coli?
What is the primary function of Type I restriction endonucleases?
What is the primary function of Type I restriction endonucleases?
What is the distinguishing feature of blunt end fragments produced by restriction enzymes?
What is the distinguishing feature of blunt end fragments produced by restriction enzymes?
Which co-factor is commonly required for Type I restriction endonucleases to function?
Which co-factor is commonly required for Type I restriction endonucleases to function?
What is the recognition sequence length for Type II restriction endonucleases?
What is the recognition sequence length for Type II restriction endonucleases?
How do restriction enzymes create new molecules from DNA fragments?
How do restriction enzymes create new molecules from DNA fragments?
In what way do Type III restriction endonucleases differ from Type II enzymes?
In what way do Type III restriction endonucleases differ from Type II enzymes?
What is the main function of restriction endonucleases?
What is the main function of restriction endonucleases?
Who were the scientists awarded the 1978 Nobel Prize for Physiology or Medicine for their work on restriction enzymes discovery and characterization?
Who were the scientists awarded the 1978 Nobel Prize for Physiology or Medicine for their work on restriction enzymes discovery and characterization?
What type of nucleotide sequence do restriction enzymes recognize before making cuts in DNA?
What type of nucleotide sequence do restriction enzymes recognize before making cuts in DNA?
Which of the following statements is true about the subunits of restriction enzymes?
Which of the following statements is true about the subunits of restriction enzymes?
What do bacteria use restriction enzymes for?
What do bacteria use restriction enzymes for?
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