Applications of Restriction Enzymes Quiz
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Questions and Answers

What is the main function of restriction enzymes in gene cloning experiments?

  • Determining nucleotide sequences
  • Increasing the rate of DNA replication
  • Integrating genes into a host organism
  • Cutting DNA into smaller strands for analysis (correct)

    • Which of the following is NOT an application of restriction enzymes?

  • Producing insulin for diabetics using E.coli
  • Creating recombinant DNA
  • Cloning DNA molecules
  • Studying protein synthesis mechanisms (correct)

    • What is the significance of recognition sites like Eco Rl in restriction enzyme technology?

  • They regulate gene expression
  • They are specific sites where restriction enzymes cut DNA (correct)
  • They indicate the start of a gene sequence
  • They help in DNA replication

    • In the context of restriction enzymes, what does the term 'RFLP' stand for?

    <p>Restriction Fragment Length Polymorphism</p> Signup and view all the answers

    How do restriction enzymes contribute to the large-scale production of human insulin using E.coli?

    <p>They cut the human insulin gene and insert it into E.coli plasmids</p> Signup and view all the answers

    What is the primary function of Type I restriction endonucleases?

    <p>Cleave the DNA about 1000 bp away from a specific end of the recognition site</p> Signup and view all the answers

    What is the distinguishing feature of blunt end fragments produced by restriction enzymes?

    <p>They can be joined to any other DNA fragment with blunt ends</p> Signup and view all the answers

    Which co-factor is commonly required for Type I restriction endonucleases to function?

    <p>Magnesium (Mg2+)</p> Signup and view all the answers

    What is the recognition sequence length for Type II restriction endonucleases?

    <p>15 bp</p> Signup and view all the answers

    How do restriction enzymes create new molecules from DNA fragments?

    <p>By combining DNA fragments with complimentary sticky ends</p> Signup and view all the answers

    In what way do Type III restriction endonucleases differ from Type II enzymes?

    <p>Cleavage site position relative to the target sequence</p> Signup and view all the answers

    What is the main function of restriction endonucleases?

    <p>Produce internal cuts in DNA molecules</p> Signup and view all the answers

    Who were the scientists awarded the 1978 Nobel Prize for Physiology or Medicine for their work on restriction enzymes discovery and characterization?

    <p>Werner Arber, Daniel Nathans, and Hamilton O.Smith</p> Signup and view all the answers

    What type of nucleotide sequence do restriction enzymes recognize before making cuts in DNA?

    <p>Recognition or restriction sites</p> Signup and view all the answers

    Which of the following statements is true about the subunits of restriction enzymes?

    <p>They are composed of amino acids</p> Signup and view all the answers

    What do bacteria use restriction enzymes for?

    <p>To resist viral attack</p> Signup and view all the answers

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