Restriction Enzymes Quiz

What is the function of restriction enzymes in gene cloning and recombinant DNA technology?

What role do DNA ligases play in genetic engineering?

What is the primary responsibility of DNA polymerases in genetic engineering?

What is the significance of the single-stranded overhangs created by restriction enzymes?

What is the function of reverse transcriptases in genetic engineering?

Restriction Enzymes (Endonucleases) - are used to cut DNA at specific recognition sites known as restriction sites - they cleave the DNA at these sites creating fragments with sticky ends single stranded overhangs that can be Easily joined with other DNA fragments - restriction enzymes are crucial for gene cloning and recombinant DNA technology after DNA has been cut by restriction enzymes DNA ligases - play the role of genetic glue - they catalyze the joining of DNA fragments by forming phosphodiester bonds between the sugar phosphate backbones of adjacent DNA strands - DNA ligases are essential for Creating recombinant DNA molecules DNA polymerases - are enzymes responsible for DNA replication copying the genetic material during cell division - in genetic engineering DNA polymerases are used for processes like polymerase Chain Reaction PCR which amplifies specific DNA sequences and DNA sequencing allowing researchers to read the genetic code Reverse Transcriptase’s - are used to creat.

Restriction enzymes (Endonucleases) are used to cut DNA at specific recognition sites known as restriction sites - they cleave the DNA at these sites creating fragments with ______

DNA ligases play the role of genetic ______

DNA polymerases are enzymes responsible for DNA replication copying the genetic material during cell ______

Reverse Transcriptase’s are used to ______

DNA ligases are essential for creating recombinant DNA ______