Recombinant DNA Technology

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Why did early attempts to manipulate the genetic properties of cells fail?

Only a limited number of bacterial species could take up DNA spontaneously.

What is required for foreign DNA to be maintained in a recipient cell?

It must replicate autonomously or integrate into the recipient genome.

In recombinant DNA technology, what term is used for DNA molecules that can replicate themselves?

Replicons

What characteristic is essential for vectors used in biotechnology?

They must be easily isolated as intact molecules.

How do restriction enzymes contribute to recombinant DNA technology?

They cleave DNA at specific sites.

Explore the basics of recombinant DNA technology, including the challenges of early attempts, requirements for maintaining foreign DNA, and key concepts like vectors and restriction enzymes.

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