Recombinant DNA Technology
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Questions and Answers

Why did early attempts to manipulate the genetic properties of cells fail?

  • Cells did not respond to the presence of foreign proteins.
  • Most cells were unable to divide rapidly enough.
  • Only a limited number of bacterial species could take up DNA spontaneously. (correct)
  • Cells rejected foreign DNA due to immune responses.

    • What is required for foreign DNA to be maintained in a recipient cell?

  • It must replicate autonomously or integrate into the recipient genome. (correct)
  • It must evade all immune responses.
  • It must produce essential proteins.
  • It must be protected by viral proteins.

    • In recombinant DNA technology, what term is used for DNA molecules that can replicate themselves?

  • Transposons
  • Replicons (correct)
  • Nucleotides
  • Plasmids

    • What characteristic is essential for vectors used in biotechnology?

    <p>They must be easily isolated as intact molecules. (C)</p> Signup and view all the answers

    How do restriction enzymes contribute to recombinant DNA technology?

    <p>They cleave DNA at specific sites. (C)</p> Signup and view all the answers

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