10 Questions
What determines the size of DNA fragments generated by restriction enzymes?
The frequency of occurrence of the recognition sequence
What is the purpose of restriction endonucleases in genetic engineering?
To cut specific sequences of DNA
Which part of the restriction enzyme nomenclature comes from the specific name of the organism?
First two letters of the specific name
What is illustrated by the binding of the restriction enzyme BamHI to the DNA helix in Fig. 4.1?
The close relationship between structure and function in biology
Which enzyme is derived from Escherichia coli?
EcoRI
How many base pairs are the most common recognition sequences for restriction enzymes?
Four, five, or six
How does the enzyme BamHI facilitate the hydrolysis of the phosphodiester linkages in DNA?
By wrapping around the DNA helix
Which molecular modeling software was used to generate the figure illustrating BamHI binding?
RasMol
What additional descriptors may be added to the name of a restriction enzyme?
Bacterial strain and presence of extrachromosomal elements
What characteristic of restriction endonucleases gives them value in genetic engineering?
Their specificity to recognize and cut specific DNA sequences
Learn about the essential tools and techniques used in genetic engineering, including cutting and joining DNA from different sources and modifying DNA molecules.
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