Genetic Engineering Chapter 4
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Questions and Answers

What determines the size of DNA fragments generated by restriction enzymes?

  • The bacterium from which the enzyme is purified
  • The temperature at which the enzyme operates
  • The enzyme's efficiency
  • The frequency of occurrence of the recognition sequence (correct)
  • What is the purpose of restriction endonucleases in genetic engineering?

  • To inhibit DNA replication
  • To amplify DNA sequences
  • To synthesize new DNA strands
  • To cut specific sequences of DNA (correct)
  • Which part of the restriction enzyme nomenclature comes from the specific name of the organism?

  • Presence of extrachromosomal elements
  • First two letters of the specific name (correct)
  • Strain indicator
  • First letter of the generic name
  • What is illustrated by the binding of the restriction enzyme BamHI to the DNA helix in Fig. 4.1?

    <p>The close relationship between structure and function in biology</p> Signup and view all the answers

    Which enzyme is derived from Escherichia coli?

    <p>EcoRI</p> Signup and view all the answers

    How many base pairs are the most common recognition sequences for restriction enzymes?

    <p>Four, five, or six</p> Signup and view all the answers

    How does the enzyme BamHI facilitate the hydrolysis of the phosphodiester linkages in DNA?

    <p>By wrapping around the DNA helix</p> Signup and view all the answers

    Which molecular modeling software was used to generate the figure illustrating BamHI binding?

    <p>RasMol</p> Signup and view all the answers

    What additional descriptors may be added to the name of a restriction enzyme?

    <p>Bacterial strain and presence of extrachromosomal elements</p> Signup and view all the answers

    What characteristic of restriction endonucleases gives them value in genetic engineering?

    <p>Their specificity to recognize and cut specific DNA sequences</p> Signup and view all the answers

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