Recombinant DNA Technology

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If a bacterium does not contain a plasmid, what phenotype will it express in the presence of an antibiotic for which a recombinant plasmid provides resistance?

It will be sensitive to the antibiotic. (correct)
It will replicate faster than bacteria containing the recombinant plasmid.
It will mutate to gain resistance to the antibiotic.
It will exhibit resistance to the antibiotic due to horizontal gene transfer.

Viruses cannot be used as a cloning vehicle to incorporate genetic material into a host organism.

False (B)

The restriction endonuclease EcoRI recognizes a specific palindromic sequence in DNA. What is the sequence in question?

GAATTC

The process by which bacterial walls become temporarily permeable, allowing a plasmid to enter, is called ______.

metamorphosis Signup and view all the answers

Match the steps involved in constructing a genomic library.

DNA Fragmentation = Genomic DNA is broken down into smaller, manageable pieces. Vector Insertion = DNA fragments are inserted into cloning vectors like plasmids or bacteriophages. Recombinant DNA Creation = Vectors containing foreign DNA are created. Host Introduction = Recombinant vectors are introduced into host cells. Library Screening = The library is screened to identify clones containing the gene of interest. Signup and view all the answers

Which of the following poses the greatest challenge in creating a functional recombinant DNA molecule?

Maintaining the stability and expression of the foreign gene within the host cell across multiple generations. (D) Signup and view all the answers

Genetic engineering's primary goals are limited to improving human health and agricultural practices.

False (B) Signup and view all the answers

Describe a hypothetical scenario where the use of a specific restriction endonuclease, different from EcoRI, might be necessary for creating a recombinant DNA molecule and explain the rationale behind the choice.

If the gene of interest contains EcoRI restriction sites within its sequence, another restriction enzyme with a different recognition sequence must be used to prevent the gene from being cut into multiple, non-functional fragments. Signup and view all the answers

The process of producing a large number of identical molecules, cells, or organisms is referred to as ______.

cloning Signup and view all the answers

Match the following steps of recombinant DNA technology with their correct description:

Making recombinant DNA = Cutting donor DNA with restriction endonucleases and combining it with a cloning vector. Transfer to host cell = Introducing the recombinant DNA molecule into a bacterial cell. Selection of host cells = Identifying and isolating host cells that have successfully incorporated the recombinant DNA. Gene integration = Incorporating the new gene into DNA. Signup and view all the answers

Recombinant DNA

Artificial DNA containing genes from two or more organisms.

Genetic Engineering

Techniques used to modify the genetic material of organisms.