11 Questions
Which of the following is true about recombinant DNA technology?
Recombinant DNA molecules are formed by combining genetic material from multiple sources
What is the term used to describe DNA molecules that have been created by combining fragments from different sources?
Chimeric DNA
Which of the following is required for the expression of foreign proteins using recombinant DNA technology?
Specialized expression vectors
Which method involves cutting and pasting DNA sequences?
Restriction enzyme/ligase cloning
Which method involves replicating DNA within a living cell?
Molecular cloning
Which process requires the transfection of suitable host cells?
DNA expression
What is the purpose of a cloning vector in molecular cloning?
To replicate within a living cell
Which of the following is NOT an application of recombinant DNA technology?
Sequencing genes
What is the advantage of using recombinant human insulin over insulin obtained from animal sources?
It does not stimulate the immune system
What is the most common application of recombinant DNA technology?
Basic research
What is the function of recombinant chymosin in cheese production?
To coagulate the milk proteins in the cheese
Study Notes
Recombinant DNA Technology
- Recombinant DNA molecules are created by combining fragments from different sources, a process known as molecular cloning.
Key Requirements
- Expression of foreign proteins using recombinant DNA technology requires transcription and translation machinery.
DNA Manipulation Methods
- Restriction enzymes are used to cut DNA sequences into fragments, which can then be combined using DNA ligase, a process known as "cutting and pasting" DNA sequences.
- Polymerase chain reaction (PCR) is a method that involves replicating DNA within a living cell.
Molecular Cloning
- The process of transfecting suitable host cells with recombinant DNA molecules is necessary for molecular cloning.
- Cloning vectors, such as plasmids, are used to replicate and maintain recombinant DNA molecules in host cells.
Applications and Advantages
- Recombinant DNA technology has many applications, including the production of recombinant human insulin, which has the advantage of being more consistent and reliable than insulin obtained from animal sources.
- The most common application of recombinant DNA technology is the production of therapeutic proteins.
- Recombinant chymosin is used in cheese production to improve the efficiency and quality of the cheese-making process.
Exceptions
- Gene therapy is NOT an application of recombinant DNA technology.
Test your knowledge on Recombinant DNA (rDNA) molecules and genetic recombination techniques with this quiz. Explore the creation of DNA sequences by combining fragments from different sources and learn about the applications of recombinant DNA in genetic engineering.
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