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Questions and Answers
What is the purpose of injecting 30% urethane into the rabbit?
What is the purpose of injecting 30% urethane into the rabbit?
Which step follows the injection of urethane?
Which step follows the injection of urethane?
When inserting the arterial cannula, where is the incision made?
When inserting the arterial cannula, where is the incision made?
What is the purpose of ligating the left common carotid artery before cannulation?
What is the purpose of ligating the left common carotid artery before cannulation?
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What should be done to prevent blood return to the heart when inserting a ductus venosus?
What should be done to prevent blood return to the heart when inserting a ductus venosus?
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What is observed after injecting liquid paraffin into the vein?
What is observed after injecting liquid paraffin into the vein?
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What should be done after death to further understand the effects of the procedures?
What should be done after death to further understand the effects of the procedures?
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What is the primary function of connecting the cannula to a transducer?
What is the primary function of connecting the cannula to a transducer?
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Study Notes
Rabbit Experiment Methods and Procedures
- Rabbit Weight: Details not provided.
- Anesthesia: Inject 30% urethane from marginal ear vein slowly (3ml/kg).
- Surgical Preparation: Fix rabbit on operating table, shave neck, and create a 5-7cm incision along the neck's midline.
- Arterial Cannulation: Separate the left common carotid artery, insert an arterial cannula into it, and connect to a transducer to measure blood pressure.
- Arterial Cannula Insertion Method: Ligate the end of the artery furthest from the heart with a silk thread. Block the end of the artery closest to the heart. Cross a thread between the knot and clip under the artery. Make an inclined cut towards the heart on the artery with ophthalmological scissors (half the artery's diameter). Insert arterial cannula into the cut, secure with a thread, avoiding slippage.
- Venous Cannulation (ductus venosus): Cross a thread under vena jugularis externa and lift the end furthest from the heart. Make an inclined cut towards the heart with ophthalmological scissors(half the vein's diameter). Insert the ductus venosus into the cut, and lay down the thread under the vein to secure blood return.
- Heparin Injection: Inject heparin solution into the ear vein to prevent clotting.
- Blood Pressure Monitoring: Open the computer and monitor blood pressure.
- Paraffin Injection: Inject liquid paraffin (0.5-1ml) into the ear vein to observe thromboembolism and the effect on blood pressure.
- Normal Saline Injection: Inject normal saline into the heart rapidly from the ductus venosus, and observe the change in central venous pressure (CVP).
- Post-mortem Examination: After death, open the chest cavity and observe the heart, liver, and ascites for any changes.
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Description
This quiz covers the detailed methods and procedures involved in conducting experiments on rabbits, specifically focusing on anesthesia, surgical preparation, and cannulation techniques. Understand the step-by-step processes for arterial and venous cannulation, ensuring proper execution for experimental integrity.
