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Questions and Answers
What is the purpose of ligating the left common carotid artery during arterial cannulation?
What is the purpose of ligating the left common carotid artery during arterial cannulation?
Which technique is used to insert the ductus venosus into the right atrium?
Which technique is used to insert the ductus venosus into the right atrium?
What should be done after injecting heparin solution into the vein?
What should be done after injecting heparin solution into the vein?
What is the function of connecting the arterial cannula to a transducer?
What is the function of connecting the arterial cannula to a transducer?
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Which step is performed immediately before making an incision on the skin?
Which step is performed immediately before making an incision on the skin?
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After the death of the subject, which area should be examined?
After the death of the subject, which area should be examined?
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What is the initial step when inserting the arterial cannula into the artery?
What is the initial step when inserting the arterial cannula into the artery?
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What is the volume of urethane injected for anesthesia per kg of body weight?
What is the volume of urethane injected for anesthesia per kg of body weight?
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Study Notes
Rabbit Experiment Methods and Procedures
- Rabbit Weight: Measured
- Anesthesia: Inject 30% urethane from marginal ear vein slowly (3ml/kg)
- Preparation: Fix rabbit on operating table, shave neck fur, cut 5-7cm incision on skin along cervical midline
- Arterial Cannulation: Separate left common carotid artery, insert arterial cannula into it, connect to transducer to measure blood pressure
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Arterial Cannula Insertion Method:
- Ligate left common carotid artery near head with silk thread
- Block artery near heart with clip
- Cross thread between knot and clip under artery
- Use scissors to make an incision towards heart on artery (half artery diameter)
- Insert cannula, ligate silk thread for secure cannula placement (prevent slippage)
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Ductus Venosus Insertion:
- Cross a thread under vena jugularis externa
- Lift end of vena far from heart (avoid blood return)
- Make incision towards heart on vein (half vein diameter)
- Insert ductus venosus for 5-6cm to right atrium, ensure blood return with thread under vein
- Heparin Injection: Inject heparin solution into vein edge of ear to prevent clotting
- Blood Pressure Measurement: Observe blood pressure using computer and transducer
- Paraffin Injection: Inject 0.5-1ml liquid paraffin into vein edge of ear to induce thromboembolism and observe blood pressure change
- Normal Saline Injection: Inject normal saline into heart to determine change in central venous pressure (CVP)
- Post-Mortem Observation: Open chest and abdomen, observe heart, liver, and ascites after death.
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Description
This quiz covers the methods and procedures used in conducting rabbit experiments, focusing on anesthesia, arterial cannulation, and ductus venosus insertion techniques. It provides detailed steps for accurately measuring and managing various experimental parameters during the procedure.
