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Questions and Answers

What indicates damage to cells rich in aminotransferases?

  • Elevated plasma levels of bile acids
  • Elevated plasma levels of aminotransferases (correct)
  • Decreased plasma levels of bilirubin
  • Decreased plasma levels of amino acids
  • Which organ primarily features L-amino acid oxidase activity?

  • Spleen
  • Heart
  • Liver (correct)
  • Lungs
  • What is the product of the reaction catalyzed by L-amino acid oxidase?

  • Lamino acid
  • NADH
  • Urea
  • α-keto acid (correct)
  • Which enzyme is primarily responsible for the oxidative deamination of L-glutamate?

    <p>L-glutamate dehydrogenase</p> Signup and view all the answers

    Which enzyme is considered to have low activity and is found only in liver and kidneys?

    <p>L-amino acid oxidase</p> Signup and view all the answers

    What is NOT a product of L-amino acid oxidase activity?

    <p>Carbon dioxide</p> Signup and view all the answers

    What role does L-glutamate dehydrogenase play in amino acid metabolism?

    <p>Deamination of most amino acids</p> Signup and view all the answers

    Which of the following is a key characteristic of L-glutamate dehydrogenase?

    <p>It requires NAD(P)+ for its activity.</p> Signup and view all the answers

    What is the primary role of L-Glutamate dehydrogenase in amino acid metabolism?

    <p>To catalyze the oxidative deamination of glutamate</p> Signup and view all the answers

    Which process is primarily involved in producing free ammonia from amino acids?

    <p>Transdeamination mechanism</p> Signup and view all the answers

    Which of the following amino acids is specifically acted upon by serine dehydratase?

    <p>Serine</p> Signup and view all the answers

    What is the end product of the glycine cleavage system?

    <p>THF (Tetrahydrofolate)</p> Signup and view all the answers

    How do reductive deamination processes mainly occur?

    <p>By intestinal bacterial action on amino acids</p> Signup and view all the answers

    Which method specifically converts glutamine into glutamate?

    <p>Hydrolytic deamination via glutaminase</p> Signup and view all the answers

    In the process of transdeamination, which sequence of reactions occurs?

    <p>Transamination followed by oxidative deamination</p> Signup and view all the answers

    Which of the following is not involved in the deamination of amino acids?

    <p>Oxidative phosphorylation</p> Signup and view all the answers

    What is the primary anabolic pathway for ammonia following deamination of amino acids?

    <p>Formation of non-essential amino acids</p> Signup and view all the answers

    Which compound is primarily responsible for the removal of ammonia in the brain?

    <p>Glutamine</p> Signup and view all the answers

    What percentage of total urinary ammonia is derived from the degradation of glutamine in the kidneys?

    <p>80%</p> Signup and view all the answers

    Which pathway is quantitatively the most important for ammonia disposal in the liver?

    <p>Production of urea</p> Signup and view all the answers

    What is produced by the deamination of glutamine in the liver?

    <p>Glutamate and ammonia</p> Signup and view all the answers

    What role do the kidneys play in ammonia excretion?

    <p>They degrade glutamine to release ammonia.</p> Signup and view all the answers

    What effect does liver cirrhosis have on ammonia production in the intestines?

    <p>Increases ammonia production.</p> Signup and view all the answers

    Which enzyme synthesizes glutamine from glutamate and ammonia?

    <p>Glutamine synthetase</p> Signup and view all the answers

    What is the primary role of transamination reactions in amino acid metabolism?

    <p>Biosynthesis of nonessential amino acids</p> Signup and view all the answers

    Which cofactor is essential for the transamination reaction to occur?

    <p>Pyridoxal phosphate (PLP)</p> Signup and view all the answers

    Which amino acid serves as the major amino group acceptor in transamination reactions?

    <p>α-Ketoglutarate</p> Signup and view all the answers

    Which of the following is NOT a common characteristic of transaminases?

    <p>Transaminases are located in mitochondria only</p> Signup and view all the answers

    Which amino acids are generally excluded from transamination reactions?

    <p>Proline, Threonine, and Lysine</p> Signup and view all the answers

    What is a key outcome of the reactions catalyzed by alanine aminotransferase (ALT)?

    <p>Formation of pyruvate and glutamate</p> Signup and view all the answers

    What does the activity of aminotransferases in plasma generally indicate?

    <p>Release of cellular contents during normal turnover</p> Signup and view all the answers

    Which two aminotransferases are considered the most important and active in transamination reactions?

    <p>Alanine aminotransferase and aspartate aminotransferase</p> Signup and view all the answers

    What compounds are combined to form carbamoyl phosphate in the urea cycle?

    <p>Free ammonium ion and bicarbonate</p> Signup and view all the answers

    How many ATP molecules are expended in the formation of carbamoyl phosphate?

    <p>2 ATP</p> Signup and view all the answers

    Which enzyme catalyzes the conversion of aspartate in the urea cycle?

    <p>Argininosuccinate synthetase</p> Signup and view all the answers

    What is the byproduct produced alongside urea in the urea cycle?

    <p>Fumarate</p> Signup and view all the answers

    Which step in the urea cycle involves the transformation of argininosuccinate?

    <p>The cleavage of argininosuccinate by argininosuccinase</p> Signup and view all the answers

    What is the primary role of the enzyme ornithine transcarbamoylase in the urea cycle?

    <p>To catalyze the reaction of carbamoyl phosphate and ornithine</p> Signup and view all the answers

    Which overall reaction is represented by the urea cycle?

    <p>NH3 + CO2 + 3ATP → Urea + Fumarate + 2ADP + AMP + PPi</p> Signup and view all the answers

    Which compound is transported across the mitochondrial membrane by the ornithine-citrulline antiporter?

    <p>Citrulline</p> Signup and view all the answers

    During the urea cycle, what happens to fumarate?

    <p>It is formed alongside urea</p> Signup and view all the answers

    What is generated when arginase acts on arginine in the urea cycle?

    <p>Ornithine</p> Signup and view all the answers

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