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Quantitative Analysis of Carbohydrates

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Questions and Answers

What is the aim of the Fehling method in sugar estimation?

To calculate the pH level of glucose solutions

To determine the boiling point of water

To identify different types of carbohydrates

To measure the concentration of sugar in a solution (correct)

Why should the Fehling reagent not be boiled during the heating process?

To increase the reaction rate of the titration

To allow for better color change detection

To avoid the formation of toxic gases

To prevent the evaporation of water from the solution (correct)

What is the end point indicator when performing the titration with Fehling's reagent?

A yellow solid precipitate

A clear solution with no precipitate

A blue color change

A reddish brown precipitate (correct)

How much glucose corresponds to 5ml of Fehling solution?

<p>0.067 gm glucose</p> Signup and view all the answers

What formula is used to calculate the amount of glucose in 100ml of solution from the end point?

<p>$X = (0.067 * 100)/E.P$</p> Signup and view all the answers

Study Notes

Quantitative Analysis of Carbohydrates

Aim: Determine the grams of glucose dissolved in a solution using the Fehling method.

Procedure

Step 1: Prepare the Fehling reagent by mixing 2.5ml of Fehling A and 2.5ml of Fehling B with 5 ml of distilled water
Step 2: Heat the solution on a hot plate to 70-80°C
- Note: Do not heat to boiling to prevent water evaporation from the solution
Step 3: Titrate the sugar solution with the Fehling reagent
Step 4: Record the endpoint, which is a reddish-brown precipitate.
Step 5: Repeat the process three times and calculate the average value.

Calculation

5ml of Fehling reagent equals 0.067g of glucose
The endpoint of the titration is equivalent to 0.067g of glucose
100ml of solution containing Xg of glucose
X = (0.067 x 100)/endpoint value (in ml) = g per 100ml of sugar solution

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Description

This quiz focuses on the quantitative analysis of glucose using the Fehling method. Participants will learn the steps involved in preparing the Fehling reagent, conducting titrations, and calculating the concentration of glucose in a solution. It's essential for students studying carbohydrate analysis in chemistry.