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Qualitative Analysis of Carbohydrates

Created by

@ProsperousLasVegas

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Questions and Answers

What is the primary aim of using the Fehling method in the quantitative analysis of carbohydrates?

To determine the grams of glucose dissolved in the solution (correct)

To identify the presence of reducing sugars other than glucose

To evaluate the total carbohydrate content in a sample

To measure the concentration of fructose in the solution

Why is it essential to heat the Fehling reagent to a temperature of 70-80°C instead of boiling?

To ensure maximum solubility of glucose

To enhance the reactivity of the reagents used

To prevent the evaporation of water from the solution (correct)

To control the viscosity of the reagent mixture

In the calculation of glucose concentration, what does 'E.P' represent?

Estimated precision of the reagents

Excess glucose present in solution

Error percentage in measurements

End point of the titration (correct)

What is the expected outcome at the end point of the titration using the Fehling method?

<p>A reddish-brown precipitate indicating completion</p> Signup and view all the answers

Which of the following correctly represents the relationship between glucose amount and solution volume as per the calculation provided?

<p>X = (0.067 x 100) / E.P</p> Signup and view all the answers

Study Notes

Qualitative Analysis of Carbohydrates

Aim: Determine the grams of glucose in solution using Fehling's method.

Fehling's Method Procedure

Step 1: Prepare Fehling's reagent; combine 2.5ml of Fehling A and 2.5ml of Fehling B, and 5ml total.
Step 2: Heat the solution to 70-80°C on a hotplate. Heat gently to avoid boiling, preventing water evaporation.
Step 3: Titrate the solution against the sugar solution.
Step 4: The endpoint is when the mixture turns a reddish-brown precipitate.
Step 5: Repeat the titration three times and average the results.

Calculations

5ml Fehling reagent equals 0.067g of glucose
End point of the titration corresponds to 0.067g glucose
To find the amount of glucose in 100ml of solution, use the formula X = (0.067 x 100)/End Point, where X equals the amount of glucose in grams per 100ml.

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Description

Explore the qualitative analysis of carbohydrates through Fehling's method. This quiz covers the procedure, calculations involved, and the titration process to determine glucose concentration in a solution. Test your understanding of carbohydrate analysis techniques and their applications.