Qualitative Analysis of Carbohydrates
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Questions and Answers

What is the primary aim of using the Fehling method in the quantitative analysis of carbohydrates?

  • To determine the grams of glucose dissolved in the solution (correct)
  • To identify the presence of reducing sugars other than glucose
  • To evaluate the total carbohydrate content in a sample
  • To measure the concentration of fructose in the solution
  • Why is it essential to heat the Fehling reagent to a temperature of 70-80°C instead of boiling?

  • To ensure maximum solubility of glucose
  • To enhance the reactivity of the reagents used
  • To prevent the evaporation of water from the solution (correct)
  • To control the viscosity of the reagent mixture
  • In the calculation of glucose concentration, what does 'E.P' represent?

  • Estimated precision of the reagents
  • Excess glucose present in solution
  • Error percentage in measurements
  • End point of the titration (correct)
  • What is the expected outcome at the end point of the titration using the Fehling method?

    <p>A reddish-brown precipitate indicating completion</p> Signup and view all the answers

    Which of the following correctly represents the relationship between glucose amount and solution volume as per the calculation provided?

    <p>X = (0.067 x 100) / E.P</p> Signup and view all the answers

    Study Notes

    Qualitative Analysis of Carbohydrates

    • Aim: Determine the grams of glucose in solution using Fehling's method.

    Fehling's Method Procedure

    • Step 1: Prepare Fehling's reagent; combine 2.5ml of Fehling A and 2.5ml of Fehling B, and 5ml total.
    • Step 2: Heat the solution to 70-80°C on a hotplate. Heat gently to avoid boiling, preventing water evaporation.
    • Step 3: Titrate the solution against the sugar solution.
    • Step 4: The endpoint is when the mixture turns a reddish-brown precipitate.
    • Step 5: Repeat the titration three times and average the results.

    Calculations

    • 5ml Fehling reagent equals 0.067g of glucose
    • End point of the titration corresponds to 0.067g glucose
    • To find the amount of glucose in 100ml of solution, use the formula X = (0.067 x 100)/End Point, where X equals the amount of glucose in grams per 100ml.

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    Description

    Explore the qualitative analysis of carbohydrates through Fehling's method. This quiz covers the procedure, calculations involved, and the titration process to determine glucose concentration in a solution. Test your understanding of carbohydrate analysis techniques and their applications.

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