Quantitative Analysis of Carbohydrates PDF
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Uploaded by ProsperousLasVegas
Faculty of Medicine - Fayoum University
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Summary
This document describes a quantitative analysis of carbohydrates using the Fehling method. The procedure involves estimations of sugar in a solution. The calculation is also described to get the concentration.
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Quantitative analysis of carbohydrates (1): Estimation of sugar by fehling method: Aim: To know the number of grams of glucose dissolved in solution. Procedure 1-Take 5ml fehling reagent (2.5ml F.A + 2.5ml F.B). 2- Heat over hot plate till 70-80oC. Note; Heat not boil...
Quantitative analysis of carbohydrates (1): Estimation of sugar by fehling method: Aim: To know the number of grams of glucose dissolved in solution. Procedure 1-Take 5ml fehling reagent (2.5ml F.A + 2.5ml F.B). 2- Heat over hot plate till 70-80oC. Note; Heat not boiling due to: *Preventing the evaporation of water from solution. 3- Titration against sugar solution. 4- End point is reddish brown ppt. 5- Repeat 3 times and take the average. Calculation 5ml Feh = 0.067gm glucose 5ml Feh = End point So, End point = 0.067gm glucose 100ml soln = X gm glucose X= (0.067x100)/E.P gm/100ml sugar solution
