Quantitation of Nucleic Acids Quiz
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Questions and Answers

Which method is commonly used for quantitation of nucleic acids?

  • Western blot method
  • PCR amplification method
  • Immunofluorescence method
  • Gel based method (correct)

    • What is the purpose of spectrophotometric method in nucleic acid quantitation?

  • Measuring absorbance at specific wavelengths (correct)
  • Visualizing the nucleic acids under UV light
  • Counting the number of nucleotides
  • Determining the sequence of nucleic acids

    • What can interfere with accurate nucleic acid quantitation using spectrophotometric method?

  • High salt concentration
  • Presence of primers
  • Temperature fluctuations
  • Contaminants such as proteins and phenol (correct)

    • Which method is commonly used for quantitation of nucleic acids?

    <p>Gel-based method</p> Signup and view all the answers

    What is the main principle of spectrophotometric method for nucleic acid quantitation?

    <p>Absorbance of UV light by nucleic acids</p> Signup and view all the answers

    Why is it important to accurately quantify nucleic acids?

    <p>To ensure optimal performance in downstream applications</p> Signup and view all the answers

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