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Questions and Answers
Which method is commonly used for quantitation of nucleic acids?
Which method is commonly used for quantitation of nucleic acids?
- Western blot method
- PCR amplification method
- Immunofluorescence method
- Gel based method (correct)
What is the purpose of spectrophotometric method in nucleic acid quantitation?
What is the purpose of spectrophotometric method in nucleic acid quantitation?
- Measuring absorbance at specific wavelengths (correct)
- Visualizing the nucleic acids under UV light
- Counting the number of nucleotides
- Determining the sequence of nucleic acids
What can interfere with accurate nucleic acid quantitation using spectrophotometric method?
What can interfere with accurate nucleic acid quantitation using spectrophotometric method?
- High salt concentration
- Presence of primers
- Temperature fluctuations
- Contaminants such as proteins and phenol (correct)
Which method is commonly used for quantitation of nucleic acids?
Which method is commonly used for quantitation of nucleic acids?
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What is the main principle of spectrophotometric method for nucleic acid quantitation?
What is the main principle of spectrophotometric method for nucleic acid quantitation?
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Why is it important to accurately quantify nucleic acids?
Why is it important to accurately quantify nucleic acids?
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