Protein Quantitation Techniques: Colorimetric Assays
20 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary structure of a protein?

  • Long polymer with 50 amino acids or more
  • Short chain of amino acids
  • Folded, functional molecule composed of one polypeptide
  • Amide bond formed between two amino acids (correct)
  • In which direction are proteins synthesized?

  • From the amine tail to the carboxylic acid tail (correct)
  • From the carboxylic acid tail to the amine tail
  • In a random direction
  • Simultaneously in both directions
  • What is the consequence of mutant hemoglobin being prone to aggregation in low oxygen conditions?

  • Increased deformity of red blood cells (correct)
  • Decreased deformity of red blood cells
  • Enhanced binding of oxygen to hemoglobin
  • No effect on red blood cells
  • Which term refers to a longer polymer with 50 amino acids or more, that can be in the folded state or not?

    <p>Polypeptide</p> Signup and view all the answers

    What is the principle behind the Bicinchoninic Acid (BCA) Assay for protein quantitation?

    <p>It displaces proteins bound to Cu1+</p> Signup and view all the answers

    What is a key advantage of the Bicinchoninic Acid (BCA) Assay over the Biuret Assay for protein quantitation?

    <p>It can detect lower protein concentrations than the Biuret assay</p> Signup and view all the answers

    What is the purpose of the Folin-phenol reagent in the Lowry Assay for protein quantitation?

    <p>To bind to the peptide-copper complex, producing an intense blue color</p> Signup and view all the answers

    What is the main objective of homology modeling in relation to protein structure?

    <p>To predict the unknown three-dimensional structure of a protein</p> Signup and view all the answers

    In protein quantitation, which assay is capable of detecting higher protein concentrations than the Bicinchoninic Acid (BCA) Assay?

    <p>Biuret Assay</p> Signup and view all the answers

    What is the function of hemoglobin in relation to Sickle Cell Anemia?

    <p>To bind oxygen</p> Signup and view all the answers

    What type of bonding is responsible for the formation of secondary structures in a polypeptide?

    <p>Hydrogen bonding</p> Signup and view all the answers

    In secondary structures, what part of the amino acids is involved in forming the hydrogen bonds?

    <p>Peptide backbones</p> Signup and view all the answers

    Which type of secondary structure has the R groups pointed away from the helix?

    <p>Alpha-helix</p> Signup and view all the answers

    What type of bonds are depicted by the dotted lines in the alpha-helix structure?

    <p>Hydrogen bonds</p> Signup and view all the answers

    Which of the following is NOT a type of common secondary structure?

    <p>Collagen triple helix</p> Signup and view all the answers

    In terms of diameter, which secondary structure has the largest diameter?

    <p>Pi-helix</p> Signup and view all the answers

    What type of interactions hold together the tertiary structure of a polypeptide?

    <p>Different chemical interactions including side chains and peptide backbone atoms</p> Signup and view all the answers

    Which technique is considered as the gold standard for determining the 3D structure of proteins?

    <p>X-ray crystallography</p> Signup and view all the answers

    What angles does a Ramachandran plot show for each amino acid of a protein?

    <p>Both Phi angle (Φ) and Psi angle (ψ)</p> Signup and view all the answers

    What does one dot represent on a Ramachandran plot?

    <p>One amino acid in the chain</p> Signup and view all the answers

    More Like This

    Science Quiz Protein Synthesis
    15 questions

    Science Quiz Protein Synthesis

    SolicitousPelican7010 avatar
    SolicitousPelican7010
    Protein Synthesis Overview
    11 questions
    Protein Structure and Bonds
    22 questions
    Use Quizgecko on...
    Browser
    Browser