20 Questions
What is the primary structure of a protein?
Amide bond formed between two amino acids
In which direction are proteins synthesized?
From the amine tail to the carboxylic acid tail
What is the consequence of mutant hemoglobin being prone to aggregation in low oxygen conditions?
Increased deformity of red blood cells
Which term refers to a longer polymer with 50 amino acids or more, that can be in the folded state or not?
Polypeptide
What is the principle behind the Bicinchoninic Acid (BCA) Assay for protein quantitation?
It displaces proteins bound to Cu1+
What is a key advantage of the Bicinchoninic Acid (BCA) Assay over the Biuret Assay for protein quantitation?
It can detect lower protein concentrations than the Biuret assay
What is the purpose of the Folin-phenol reagent in the Lowry Assay for protein quantitation?
To bind to the peptide-copper complex, producing an intense blue color
What is the main objective of homology modeling in relation to protein structure?
To predict the unknown three-dimensional structure of a protein
In protein quantitation, which assay is capable of detecting higher protein concentrations than the Bicinchoninic Acid (BCA) Assay?
Biuret Assay
What is the function of hemoglobin in relation to Sickle Cell Anemia?
To bind oxygen
What type of bonding is responsible for the formation of secondary structures in a polypeptide?
Hydrogen bonding
In secondary structures, what part of the amino acids is involved in forming the hydrogen bonds?
Peptide backbones
Which type of secondary structure has the R groups pointed away from the helix?
Alpha-helix
What type of bonds are depicted by the dotted lines in the alpha-helix structure?
Hydrogen bonds
Which of the following is NOT a type of common secondary structure?
Collagen triple helix
In terms of diameter, which secondary structure has the largest diameter?
Pi-helix
What type of interactions hold together the tertiary structure of a polypeptide?
Different chemical interactions including side chains and peptide backbone atoms
Which technique is considered as the gold standard for determining the 3D structure of proteins?
X-ray crystallography
What angles does a Ramachandran plot show for each amino acid of a protein?
Both Phi angle (Φ) and Psi angle (ψ)
What does one dot represent on a Ramachandran plot?
One amino acid in the chain
Test your knowledge of protein quantitation techniques such as Biuret Assay, Lowry Assay, and Bicinchoninic Acid (BCA) Assay. Understand the principles behind these colorimetric assays and how they are used to measure protein concentration.
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