Protein Quantitation Techniques: Colorimetric Assays
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Questions and Answers

What is the primary structure of a protein?

  • Long polymer with 50 amino acids or more
  • Short chain of amino acids
  • Folded, functional molecule composed of one polypeptide
  • Amide bond formed between two amino acids (correct)
  • In which direction are proteins synthesized?

  • From the amine tail to the carboxylic acid tail (correct)
  • From the carboxylic acid tail to the amine tail
  • In a random direction
  • Simultaneously in both directions
  • What is the consequence of mutant hemoglobin being prone to aggregation in low oxygen conditions?

  • Increased deformity of red blood cells (correct)
  • Decreased deformity of red blood cells
  • Enhanced binding of oxygen to hemoglobin
  • No effect on red blood cells
  • Which term refers to a longer polymer with 50 amino acids or more, that can be in the folded state or not?

    <p>Polypeptide</p> Signup and view all the answers

    What is the principle behind the Bicinchoninic Acid (BCA) Assay for protein quantitation?

    <p>It displaces proteins bound to Cu1+</p> Signup and view all the answers

    What is a key advantage of the Bicinchoninic Acid (BCA) Assay over the Biuret Assay for protein quantitation?

    <p>It can detect lower protein concentrations than the Biuret assay</p> Signup and view all the answers

    What is the purpose of the Folin-phenol reagent in the Lowry Assay for protein quantitation?

    <p>To bind to the peptide-copper complex, producing an intense blue color</p> Signup and view all the answers

    What is the main objective of homology modeling in relation to protein structure?

    <p>To predict the unknown three-dimensional structure of a protein</p> Signup and view all the answers

    In protein quantitation, which assay is capable of detecting higher protein concentrations than the Bicinchoninic Acid (BCA) Assay?

    <p>Biuret Assay</p> Signup and view all the answers

    What is the function of hemoglobin in relation to Sickle Cell Anemia?

    <p>To bind oxygen</p> Signup and view all the answers

    What type of bonding is responsible for the formation of secondary structures in a polypeptide?

    <p>Hydrogen bonding</p> Signup and view all the answers

    In secondary structures, what part of the amino acids is involved in forming the hydrogen bonds?

    <p>Peptide backbones</p> Signup and view all the answers

    Which type of secondary structure has the R groups pointed away from the helix?

    <p>Alpha-helix</p> Signup and view all the answers

    What type of bonds are depicted by the dotted lines in the alpha-helix structure?

    <p>Hydrogen bonds</p> Signup and view all the answers

    Which of the following is NOT a type of common secondary structure?

    <p>Collagen triple helix</p> Signup and view all the answers

    In terms of diameter, which secondary structure has the largest diameter?

    <p>Pi-helix</p> Signup and view all the answers

    What type of interactions hold together the tertiary structure of a polypeptide?

    <p>Different chemical interactions including side chains and peptide backbone atoms</p> Signup and view all the answers

    Which technique is considered as the gold standard for determining the 3D structure of proteins?

    <p>X-ray crystallography</p> Signup and view all the answers

    What angles does a Ramachandran plot show for each amino acid of a protein?

    <p>Both Phi angle (Φ) and Psi angle (ψ)</p> Signup and view all the answers

    What does one dot represent on a Ramachandran plot?

    <p>One amino acid in the chain</p> Signup and view all the answers

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